Anti-IGF1 Receptor antibody [EPR23027-204]

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGF1 Receptor antibody [EPR23027-204] (AB263903)

Immunohistochemical analysis of paraffin-embedded Human prostatic hyperplasia tissue labeling IGF1 Receptor with ab263903 at 1/500 dilution (1.24 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). Membranous and cytoplasmic staining on human prostatic hyperplasia (PMID : 20710042). The section was incubated with ab263903 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGF1 Receptor antibody [EPR23027-204] (AB263903)

Immunohistochemical analysis of paraffin-embedded Human bladder carcinoma tissue labeling IGF1 Receptor with ab263903 at 1/500 dilution (1.24 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). Membranous and cytoplasmic staining on human bladder carcinoma (PMID : 20710042). The section was incubated with ab263903 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGF1 Receptor antibody [EPR23027-204] (AB263903)

Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling IGF1 Receptor with ab263903 at 1/500 dilution (1.24 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). Membranous and cytoplasmic staining on human breast carcinoma (PMID : 21057462). The section was incubated with ab263903 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

• WB

Unknown

Western blot - Anti-IGF1 Receptor antibody [EPR23027-204] (AB263903)

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 10 seconds.

The expression profile & molecular weight observed is consistent with what has been described in the literature (PMID : 21807868, 28591735).

Note : the bands larger than 200kDa are Pro-IGF1R

Low expression cell line : MDA-MB-231 (PMID : 28591735).

All lanes:

Western blot - Anti-IGF1 Receptor antibody [EPR23027-204] (ab263903) at 1/100000 dilution

Lane 1:

A431 (human epidermoid carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

MDA-MB-231 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Predicted band size: 154 kDa

Observed band size: 130 kDa,200 kDa

false

• WB

Lab

Western blot - Anti-IGF1 Receptor antibody [EPR23027-204] (AB263903)

False colour image of Western blot : Anti-IGF1 Receptor antibody [EPR23027-204] staining at 1/1000 dilution, shown in green; Mouse anti-CANX [CANX/1543] (ab238078) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab263903 was shown to bind specifically to IGF1 Receptor. A band was observed at 105-125 kDa (alpha chain) in wild-type MCF7 cell lysates with no signal observed at this size in IGF1R knockout cell line. To generate this image, wild-type and IGF1R knockout MCF7 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-IGF1 Receptor antibody [EPR23027-204] (ab263903) at 1/1000 dilution

Lane 1:

Wild-type MCF7 cell lysate at 20 µg

Lane 2:

IGF1R knockout MCF7 cell lysate at 20 µg

Lane 3:

HeLa cell lysate at 20 µg

Lane 4:

HDLM-2 cell lysate at 20 µg

Observed band size: 105-125 kDa

false

• WB

Lab

Western blot - Anti-IGF1 Receptor antibody [EPR23027-204] (AB263903)

Anti-IGF1R antibody [EPR23027-204] (ab263903) staining at 1/1000 dilution, shown in green; Mouse anti-CANX [CANX/1543] (ab238078) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab263903 was shown to bind specifically to IGF1R. A band was observed at 105 kDa in wild-type HCT 116 cell lysates with no signal observed at this size in IGF1R knockout cell line. To generate this image, wild-type and IGF1R knockout HCT 116 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-IGF1 Receptor antibody [EPR23027-204] (ab263903) at 1/1000 dilution

Lanes 1 - 4:

Western blot at 20 µg

Lane 2:

Western blot - Human IGF1R knockout HCT116 cell line (<a href='/en-us/products/cell-lines/human-igf1r-knockout-hct116-cell-line-ab287509'>ab287509</a>)

Secondary

All lanes:

Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Predicted band size: 154 kDa

Observed band size: 105 kDa

false