Anti-IGF1 Receptor antibody [EPR23027-204] - BSA and Azide free

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGF1 Receptor antibody [EPR23027-204] - BSA and Azide free (AB267345)

Immunohistochemical analysis of paraffin-embedded Human prostatic hyperplasia tissue labeling IGF1 Receptor with ab263903 at 1/500 dilution (1.24 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). Membranous and cytoplasmic staining on human prostatic hyperplasia (PMID : 20710042). The section was incubated with ab263903 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab263903).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGF1 Receptor antibody [EPR23027-204] - BSA and Azide free (AB267345)

Immunohistochemical analysis of paraffin-embedded Human bladder carcinoma tissue labeling IGF1 Receptor with ab263903 at 1/500 dilution (1.24 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). Membranous and cytoplasmic staining on human bladder carcinoma (PMID : 20710042). The section was incubated with ab263903 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab263903).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGF1 Receptor antibody [EPR23027-204] - BSA and Azide free (AB267345)

Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling IGF1 Receptor with ab263903 at 1/500 dilution (1.24 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). Membranous and cytoplasmic staining on human breast carcinoma (PMID : 21057462). The section was incubated with ab263903 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab263903).

• WB

Lab

Western blot - Anti-IGF1 Receptor antibody [EPR23027-204] - BSA and Azide free (AB267345)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab263903). Anti-IGF1R antibody [EPR23027-204] (ab263903) staining at 1/1000 dilution, shown in green; Mouse anti-CANX [CANX/1543] (ab238078) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab263903 was shown to bind specifically to IGF1R. A band was observed at 105 kDa in wild-type HCT 116 cell lysates with no signal observed at this size in IGF1R knockout cell line. To generate this image, wild-type and IGF1R knockout HCT 116 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-IGF1 Receptor antibody [EPR23027-204] (<a href='/en-us/products/primary-antibodies/igf1-receptor-antibody-epr23027-204-ab263903'>ab263903</a>) at 1/1000 dilution

Lanes 1 - 4:

Western blot at 20 µg

Lane 2:

Western blot - Human IGF1R knockout HCT116 cell line (<a href='/en-us/products/cell-lines/human-igf1r-knockout-hct116-cell-line-ab287509'>ab287509</a>)

Secondary

All lanes:

Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Predicted band size: 154 kDa

Observed band size: 105 kDa

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