Rabbit Polyclonal IGF1 Receptor phospho Y1131 + Y1135 + Y1136 antibody. Suitable for WB and reacts with Recombinant full length protein - Human samples. Cited in 10 publications.
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- Genetics research 2022:15007682022Upregulation of Klotho Aggravates Insulin Resistance in Gestational Diabetes Mellitus Trophoblast Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLi Lin,Xinyu Wang,Weihua Zhao,Yaxuan ChenPubMed 36325267
- Acta biomaterialia 97:399-4082019Regulation of chitosan-mediated differentiation of human olfactory receptor neurons by insulin-like growth factor binding protein-2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTsung-Wei Huang et. al.PubMed 31421230
- International journal of molecular medicine 42:2329-23422018Treatment of type 2 diabetes mellitus via reversing insulin resistance and regulating lipid homeostasis in vitro and in vivo using cajanonic acid A.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRuiyi Yang et. al.PubMed 30226559
- Scientific reports 6:259602016Differential neuronal vulnerability identifies IGF-2 as a protective factor in ALS.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIlary Allodi et. al.PubMed 27180807
- Journal of cellular and molecular medicine 19:2397-4122015Epithelial atrophy in oral submucous fibrosis is mediated by copper (II) and arecoline of areca nut.
- Glia 60:1721-332012The role of insulin-like growth factors signaling in merlin-deficient human schwannomas.
- PloS one 7:e342742012Molecular characterisation of long-acting insulin analogues in comparison with human insulin, IGF-1 and insulin X10.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBo F Hansen et. al.PubMed 22590494
- Cell transplantation 19:369-862009Monocyte derivatives promote angiogenesis and myocyte survival in a model of myocardial infarction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesM Bouchentouf,P Paradis,K A Forner,J Cuerquis,M N Boivin,J Zheng,M R Boulassel,J P Routy,E L Schiffrin,J GalipeauPubMed 20021736
- Diabetes 58:2344-542009Foxo1 links hyperglycemia to LDL oxidation and endothelial nitric oxide synthase dysfunction in vascular endothelial cells.
- American journal of physiology. Renal physiology 294:F1315-222008Effect of renal lipid accumulation on proximal tubule Na+/H+ exchange and ammonium secretion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesI Alexandru Bobulescu et. al.PubMed 18417539
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.3
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information. Database link P08069
Reactivity data
Select an application| WB | |
|---|---|
| Human | Predicted |
| Mouse | Predicted |
| Rat | Predicted |
| Recombinant full length protein - Human | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info 1/1000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
7 products for Alternative Product
Target data
Function
Receptor tyrosine kinase which mediates actions of insulin-like growth factor 1 (IGF1). Binds IGF1 with high affinity and IGF2 and insulin (INS) with a lower affinity. The activated IGF1R is involved in cell growth and survival control. IGF1R is crucial for tumor transformation and survival of malignant cell. Ligand binding activates the receptor kinase, leading to receptor autophosphorylation, and tyrosines phosphorylation of multiple substrates, that function as signaling adapter proteins including, the insulin-receptor substrates (IRS1/2), Shc and 14-3-3 proteins. Phosphorylation of IRSs proteins lead to the activation of two main signaling pathways: the PI3K-AKT/PKB pathway and the Ras-MAPK pathway. The result of activating the MAPK pathway is increased cellular proliferation, whereas activating the PI3K pathway inhibits apoptosis and stimulates protein synthesis. Phosphorylated IRS1 can activate the 85 kDa regulatory subunit of PI3K (PIK3R1), leading to activation of several downstream substrates, including protein AKT/PKB. AKT phosphorylation, in turn, enhances protein synthesis through mTOR activation and triggers the antiapoptotic effects of IGFIR through phosphorylation and inactivation of BAD. In parallel to PI3K-driven signaling, recruitment of Grb2/SOS by phosphorylated IRS1 or Shc leads to recruitment of Ras and activation of the ras-MAPK pathway. In addition to these two main signaling pathways IGF1R signals also through the Janus kinase/signal transducer and activator of transcription pathway (JAK/STAT). Phosphorylation of JAK proteins can lead to phosphorylation/activation of signal transducers and activators of transcription (STAT) proteins. In particular activation of STAT3, may be essential for the transforming activity of IGF1R. The JAK/STAT pathway activates gene transcription and may be responsible for the transforming activity. JNK kinases can also be activated by the IGF1R. IGF1 exerts inhibiting activities on JNK activation via phosphorylation and inhibition of MAP3K5/ASK1, which is able to directly associate with the IGF1R. When present in a hybrid receptor with INSR, binds IGF1. PubMed:12138094 shows that hybrid receptors composed of IGF1R and INSR isoform Long are activated with a high affinity by IGF1, with low affinity by IGF2 and not significantly activated by insulin, and that hybrid receptors composed of IGF1R and INSR isoform Short are activated by IGF1, IGF2 and insulin. In contrast, PubMed:16831875 shows that hybrid receptors composed of IGF1R and INSR isoform Long and hybrid receptors composed of IGF1R and INSR isoform Short have similar binding characteristics, both bind IGF1 and have a low affinity for insulin.
Additional Targets
INSR phospho Y1158 + Y1162 + Y1163
Alternative names
CD221, Insulin-like growth factor 1 receptor, Insulin-like growth factor I receptor, IGF-I receptor
Recommended products
Rabbit Polyclonal IGF1 Receptor phospho Y1131 + Y1135 + Y1136 antibody. Suitable for WB and reacts with Recombinant full length protein - Human samples. Cited in 10 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.3
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information. Database link P08069
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated Insulin Receptor (IR). The final product is generated by affinity chromatography using an IR-derived peptide phosphorylated at tyrosines 1158, 1162 and 1163 (1131, 1135 and 1136 for IGF1R).
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of IR/IGF1R that contains tyrosines 1158, 1162 and 1163 of the human insulin receptor (IR) as numbered according to Ebina, et al. (1146, 1150 and 1151 according to Ullrich, et al.). The corresponding residues in the IGF1R are 1131, 1135 and 1136. The sequence is conserved in human, mouse and rat for both the IR and IGF1R.The two relevant papers are:Ebina, Y., et al. (1985) The human insulin receptor cDNA: the structural basis for hormone-activated transmembrane signalling. Cell 40(4):747-758.Ullrich, A., et al. (1985) Human insulin receptor and its relationship to the tyrosine kinase family of oncogenes. Nature 313(6005):756-761. There are a long and a short isoform of this protein. This is why we are listing 1158, 1162 and 1163 in the name (where these phospho sites in the long isoform) as well as 1146, 1150 and 1151 (for the short isoform).
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The IGF1 Receptor often referred to as IGF-1R is a transmembrane receptor protein composed of alpha and beta subunits. Studies indicate that the IGF1R protein is involved in mediating the effects of insulin-like growth factor 1 (IGF-1) and plays an important role in cellular signaling. It has a molecular weight of approximately 180 kDa and is widely expressed in various tissues with high concentrations in the liver muscle and brain. Being a tyrosine kinase receptor IGF1R plays an essential role in growth and metabolism.
- Biological function summary
IGF1R contributes to several cellular processes including cell proliferation differentiation and survival. This receptor forms a complex upon ligand binding and undergoes autophosphorylation to activate intracellular signaling cascades. IGF1R activation recruits and phosphorylates insulin receptor substrates enabling the downstream signaling pathways that promote cell growth and survival. Its function in cellular responses makes it a focal point in understanding cell biology.
- Pathways
IGF1R holds a central position in the PI3K/AKT and MAPK signaling cascades. These pathways play significant roles in cellular growth proliferation and survival. IGF1R phosphorylates various downstream effectors such as IRS-1 and Shc linking it to the activation of AKT and ERK respectively. Therefore it shares pathways with related proteins like the insulin receptor highlighting its importance in mediating similar biological responses.
- Associated diseases and disorders
IGF1R has been implicated in cancers and diabetes. Overexpression of IGF1R has been observed in various malignancies including breast cancer where it relates to resistance in treatment and poor prognosis with cell lines like MCF-7 often being studied in this context. Additionally disruptions in IGF1R signaling link to insulin resistance an important feature of type 2 diabetes. These connections make IGF1R a potential therapeutic target with IGF1R inhibitors currently under exploration to address these health challenges.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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Western blot - Anti-IGF1 Receptor (phospho Y1131+ Y1135+ Y1136) + INSR (phospho Y1158+ Y1162+ Y1163) antibody (ab5681)
IGF1 Receptor (phospho Y1131+ Y1135+ Y1136) + INSR (phospho Y1158+ Y1162+ Y1163) Western blot staining using rabbit Anti-IGF1 Receptor (phospho Y1131+ Y1135+ Y1136) + INSR (phospho Y1158+ Y1162+ Y1163) antibody
Peptide Competition - prior primary antibody incubation:
1 and 2 - no peptide,
3 - non-phosphorylated peptide corresponding to the immunogen,
4 - generic phosphotyrosine-containing peptide,
5 to 7 - phosphopeptides corresponding to other IR/IGF1R sites,
8 - phosphopeptide immunogen.
10% SDS-PAGE transferred to PVDF.
Method of detection: Pierce SuperSignal method.
The data show that only the phosphopeptide corresponding to IR/IGF1R [pYpYpY1158/1162/1163] completely blocks the antibody signal, thereby demonstrating the specificty.
All lanes: Western blot - Anti-IGF1 Receptor (phospho Y1131+ Y1135+ Y1136) + INSR (phospho Y1158+ Y1162+ Y1163) antibody (ab5681) at 1/1000 dilution
Lane 1: Unstimulated (-), CHO-T transfected with insulin receptor containing vector whole cell extract with 5% BSA TBST buffer overnight at 4°C
Lanes 2 - 8: Insulin stimulated (+), CHO-T transfected with insulin receptor containing vector whole cell extract with 5% BSA TBST buffer overnight at 4°C
SecondaryAll lanes: goat F(ab’ 2 anti-rabbit IgG HRP conjugate
Predicted band size: 154 kDa
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