Anti-IGF1+IGF2 antibody (ab9572) is a rabbit polyclonal antibody detecting IGF1 in Western Blot, IHC-P, ICC/IF, ELISA, Neut. Suitable for Human, Mouse,.
- Over 110 publications
- Trusted since 2002
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- Vaccines 11:2023In Vivo Treatment with Insulin-like Growth Factor 1 Reduces CCR5 Expression on Vaccine-Induced Activated CD4 T-Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMassimiliano Bissa,Veronica Galli,Luca Schifanella,Monica Vaccari,Mohammad Arif Rahman,Giacomo Gorini,Nicolò Binello,Sarkis Sarkis,Anna Gutowska,Isabela Silva de Castro,Melvin N Doster,Ramona Moles,Guido Ferrari,Xiaoying Shen,Georgia D Tomaras,David C Montefiori,Kombo F N'guessan,Dominic Paquin-Proulx,Pamela A Kozlowski,David J Venzon,Hyoyoung Choo-Wosoba,Matthew W Breed,Joshua Kramer,Genoveffa FranchiniPubMed 38005994
- Cells 12:2023Differential Effects of Regulatory T Cells in the Meninges and Spinal Cord of Male and Female Mice with Neuropathic Pain.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNathan T Fiore,Brooke A Keating,Yuting Chen,Sarah I Williams,Gila Moalem-TaylorPubMed 37759539
- Journal of biomedical science 30:382023Akkermansia muciniphila and its membrane protein ameliorates intestinal inflammatory stress and promotes epithelial wound healing via CREBH and miR-143/145.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHenry Wade,Kaichao Pan,Qihua Duan,Szczepan Kaluzny,Ekta Pandey,Linda Fatumoju,Viswanathan Saraswathi,Rongxue Wu,Edward N Harris,Qiaozhu SuPubMed 37287024
- Journal of cellular and molecular medicine 27:1697-17072023EGFR marks a subpopulation of dermal mesenchymal cells highly expressing IGF1 which enhances hair follicle regeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMin Chen,Zaoxu Xu,Yu Chen,Qingyang Yang,Ruiqing Lu,Yankai Dong,Xiaosong Li,Jundong Xie,Ren-He Xu,Haidong Jia,Yan Kang,Yaojiong WuPubMed 37165726
- Investigative ophthalmology & visual science 64:72023Microglial CD11b Knockout Contributes to Axonal Debris Clearance and Axonal Degradation Attenuation via IGF-1 After Acute Optic Nerve Injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiaxing Zhou,Sen Lin,Qiumei Hu,Xue Li,Xi Chen,Linlin Luo,Shiyang Ye,Wei Liu,Jian YePubMed 37145604
- iScience 26:1057232023Assessment of stromal SCD-induced drug resistance of PDAC using 3D-printed zPDX model chips.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChuntao Wu,Beiyuan Hu,Lei Wang,Xia Wu,Haitao Gu,Hanguang Dong,Jiuliang Yan,Zihao Qi,Qi Zhang,Huan Chen,Bo Yu,Sheng Hu,Yu Qian,Shuang Dong,Qiang Li,Xu Wang,Jiang LongPubMed 36590169
- International journal of molecular sciences 23:2022LIF-IGF Axis Contributes to the Proliferation of Neural Progenitor Cells in Developing Rat Cerebrum.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSho Takata,Hiromi Sakata-Haga,Hiroki Shimada,Tsuyoshi Tsukada,Daisuke Sakai,Hiroki Shoji,Mitsuhiro Tomosugi,Yuka Nakamura,Yasuhito Ishigaki,Hideaki Iizuka,Yasuhiko Hayashi,Toshihisa HattaPubMed 36361987
- International journal of medical sciences 19:1779-17862022The Potential of Transforming Growth Factor-beta Inhibitor and Vascular Endothelial Growth Factor Inhibitor as Therapeutic Agents for Uterine Leiomyoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJung Yoon Park,Boah Chae,Mee-Ran KimPubMed 36313223
- Annals of the New York Academy of Sciences 1517:203-2122022Myostatin inhibits insulin-like growth factor 1-dependent citrate secretion and osteogenesis via nicotinamide adenine dinucleotide phosphate oxidase-4 in a mouse mesenchymal stem cell line.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXin-Hua Liu,Jiang Ping Pan,William A Bauman,Christopher CardozoPubMed 36072988
- Journal of inflammation research 15:4963-49792022Therapeutic Effects of Retinoic Acid in Lipopolysaccharide-Induced Cardiac Dysfunction: Network Pharmacology and Experimental Validation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXi Wang,Chang Kong,Pan Liu,Baofeng Zhou,Wujun Geng,Hongli TangPubMed 36105385
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
- Standard buffer
- Form
- Lyophilized
- Clonality
- Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human IGF1. Database link P05019
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Reactivity data
Select an application| WB | IHC-P | sELISA | IHC-FoFr | Neut | ICC/IF | |
|---|---|---|---|---|---|---|
| Human | Expected | Tested | Expected | Expected | Expected | Expected |
| Mouse | Expected | Predicted | Predicted | Expected | Expected | Expected |
| Recombinant fragment | Not recommended | Not recommended | Not recommended | Not recommended | Expected | Not recommended |
| Recombinant fragment - Human | Tested | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Recombinant full length protein - Human | Not recommended | Not recommended | Tested | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes To detect hIGF-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes To detect hIGF-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
Species Human | Dilution info - | Notes To detect hIGF-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human, Recombinant fragment | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human, Recombinant full length protein - Human, Recombinant fragment | Dilution info - | Notes - |
sELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info 0.5-2 µg/mL | Notes Allows for the detection of at least 0.2 - 0.4 ng/well of recombinant IGF1. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human, Recombinant fragment | Dilution info - | Notes - |
IHC-FoFr
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human, Recombinant full length protein - Human, Recombinant fragment | Dilution info - | Notes - |
Neut
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment | Dilution info - | Notes To yield one-half maximal inhibition of the biological activity of hIGF-1 (5.0 ng/ml), a concentration of 0.67 - 1.0 ug/ml of this antibody is required. |
Species Mouse | Dilution info - | Notes To yield one-half maximal inhibition of the biological activity of hIGF-1 (5.0 ng/ml), a concentration of 0.67 - 1.0 ug/ml of this antibody is required. |
Species Human | Dilution info - | Notes To yield one-half maximal inhibition of the biological activity of hIGF-1 (5.0 ng/ml), a concentration of 0.67 - 1.0 ug/ml of this antibody is required. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human, Recombinant full length protein - Human | Dilution info - | Notes - |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human, Recombinant full length protein - Human, Recombinant fragment | Dilution info - | Notes - |
Associated Products
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Target data
Function
The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation (PubMed:21076856, PubMed:24132240). Ca(2+)-dependent exocytosis of IGF1 is required for sensory perception of smell in the olfactory bulb (By similarity). Acts as a ligand for IGF1R. Binds to the alpha subunit of IGF1R, leading to the activation of the intrinsic tyrosine kinase activity which autophosphorylates tyrosine residues in the beta subunit thus initiating a cascade of down-stream signaling events leading to activation of the PI3K-AKT/PKB and the Ras-MAPK pathways. Binds to integrins ITGAV:ITGB3 and ITGA6:ITGB4. Its binding to integrins and subsequent ternary complex formation with integrins and IGFR1 are essential for IGF1 signaling. Induces the phosphorylation and activation of IGFR1, MAPK3/ERK1, MAPK1/ERK2 and AKT1 (PubMed:19578119, PubMed:22351760, PubMed:23243309, PubMed:23696648). As part of the MAPK/ERK signaling pathway, acts as a negative regulator of apoptosis in cardiomyocytes via promotion of STUB1/CHIP-mediated ubiquitination and degradation of ICER-type isoforms of CREM (By similarity).
Additional Targets
IGF2
Alternative names
IBP1, IGF-1, IGF1, Insulin-like growth factor 1, Insulin-like growth factor I, Mechano growth factor, Somatomedin-C, IGF-I, MGF
Recommended products
Anti-IGF1+IGF2 antibody (ab9572) is a rabbit polyclonal antibody detecting IGF1 in Western Blot, IHC-P, ICC/IF, ELISA, Neut. Suitable for Human, Mouse,.
- Over 110 publications
- Trusted since 2002
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
- Standard buffer
- Form
- Lyophilized
- Clonality
- Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human IGF1. Database link P05019
- Purification technique
- Affinity purification Immunogen
- Light chain type
- unknown
- Reconstitution
- Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
Notes
Product Specifications
Anti-IGF1+IGF2 antibody (ab9572) is a rabbit Polyclonal antibody and is validated for use in ICC/IF, IHC-FoFr, IHC-P, Neut, WB, sELISA in human, mouse samples.
Anti-IGF1+IGF2 antibody (ab9572) specifically detects IGF1 + IFG2 (UniProt ID: P05019; Molecular weight: 8kDa) and is sold in 100 µg selling sizes.
Quality and Validation
Abcam's high quality validation processes ensure Anti-IGF1+IGF2 antibody (ab9572) has high sensitivity and specificity.
Anti-IGF1+IGF2 antibody (ab9572) has been cited over 114 times in peer reviewed journals and is trusted by the scientific community.
Although some customers have been successful in IHC we no longer batch test in this application. For an IHC validated antibody please see Anti-IGF1 Receptor antibody [EPR23027-204] ab263903
Anti-IGF1+IGF2 antibody (ab9572) has 8 independent reviews from customers.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGF1+IGF2 antibody (ab9572)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pancreas tissue labelling IGF1 with ab9572 at 1 μg/mL (45 minute incubation at room temperature). Heat mediated antigen retrieval was performed using a buffer at pH 6. An HRP-labeled polymer detection system was used with a DAB chromogen.
Sandwich ELISA - Anti-IGF1+IGF2 antibody (ab9572)
To detect hIGF-I by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IGF-I as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-I.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGF1+IGF2 antibody (ab9572)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling IGF1 with ab9572 at 1 μg/mL (45 minute incubation at room temperature). Heat mediated antigen retrieval was performed using a buffer at pH 6. An HRP-labeled polymer detection system was used with a DAB chromogen.
Western blot - Anti-IGF1+IGF2 antibody (ab9572)
All lanes: Western blot - Anti-IGF1+IGF2 antibody (ab9572) at 1/1000 dilution
Lanes 1 and 3: Mouse IGF-2 recombinant protein (aa25-91, without tag)
Lanes 2 and 4: Mouse IGF-1 recombinant protein (aa25-91, without tag)
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 22 kDa
Exposure time: 59s
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