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Anti-IGF1+IGF2 antibody (ab9572) is a rabbit polyclonal antibody detecting IGF1 in Western Blot, IHC-P, ICC/IF, ELISA, Neut. Suitable for Human, Mouse,.



- Over 110 publications

- Trusted since 2002



Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGF1+IGF2 antibody (AB9572), expandable thumbnail
  • Sandwich ELISA - Anti-IGF1+IGF2 antibody (AB9572), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGF1+IGF2 antibody (AB9572), expandable thumbnail
  • Western blot - Anti-IGF1+IGF2 antibody (AB9572), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer
Standard buffer
Form
Lyophilized
Clonality
Polyclonal

Immunogen

  • Recombinant Full Length Protein corresponding to Human IGF1. Database link P05019

Consider this alternative

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PsELISAIHC-FoFrNeutICC/IF
Human
Expected
Tested
Expected
Expected
Expected
Expected
Mouse
Expected
Predicted
Predicted
Expected
Expected
Expected
Recombinant fragment
Not recommended
Not recommended
Not recommended
Not recommended
Expected
Not recommended
Recombinant fragment - Human
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Recombinant full length protein - Human
Not recommended
Not recommended
Tested
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Recombinant fragment - Human
Dilution info
-
Notes

To detect hIGF-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Expected
Expected

Species
Mouse
Dilution info
-
Notes

To detect hIGF-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Species
Human
Dilution info
-
Notes

To detect hIGF-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Not recommended
Not recommended

Species
Recombinant full length protein - Human, Recombinant fragment
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1 µg/mL
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Predicted
Predicted

Species
Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Recombinant fragment - Human, Recombinant full length protein - Human, Recombinant fragment
Dilution info
-
Notes

-

Tested
Tested

Species
Recombinant full length protein - Human
Dilution info
0.5-2 µg/mL
Notes

Allows for the detection of at least 0.2 - 0.4 ng/well of recombinant IGF1.

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Recombinant fragment - Human, Recombinant fragment
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Recombinant fragment - Human, Recombinant full length protein - Human, Recombinant fragment
Dilution info
-
Notes

-

Expected
Expected

Species
Recombinant fragment
Dilution info
-
Notes

To yield one-half maximal inhibition of the biological activity of hIGF-1 (5.0 ng/ml), a concentration of 0.67 - 1.0 ug/ml of this antibody is required.

Species
Mouse
Dilution info
-
Notes

To yield one-half maximal inhibition of the biological activity of hIGF-1 (5.0 ng/ml), a concentration of 0.67 - 1.0 ug/ml of this antibody is required.

Species
Human
Dilution info
-
Notes

To yield one-half maximal inhibition of the biological activity of hIGF-1 (5.0 ng/ml), a concentration of 0.67 - 1.0 ug/ml of this antibody is required.

Not recommended
Not recommended

Species
Recombinant fragment - Human, Recombinant full length protein - Human
Dilution info
-
Notes

-

Expected
Expected

Species
Human, Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Recombinant fragment - Human, Recombinant full length protein - Human, Recombinant fragment
Dilution info
-
Notes

-

Associated Products

Select an associated product type

5 products for Alternative Product

Target data

Function

The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation (PubMed:21076856, PubMed:24132240). Ca(2+)-dependent exocytosis of IGF1 is required for sensory perception of smell in the olfactory bulb (By similarity). Acts as a ligand for IGF1R. Binds to the alpha subunit of IGF1R, leading to the activation of the intrinsic tyrosine kinase activity which autophosphorylates tyrosine residues in the beta subunit thus initiating a cascade of down-stream signaling events leading to activation of the PI3K-AKT/PKB and the Ras-MAPK pathways. Binds to integrins ITGAV:ITGB3 and ITGA6:ITGB4. Its binding to integrins and subsequent ternary complex formation with integrins and IGFR1 are essential for IGF1 signaling. Induces the phosphorylation and activation of IGFR1, MAPK3/ERK1, MAPK1/ERK2 and AKT1 (PubMed:19578119, PubMed:22351760, PubMed:23243309, PubMed:23696648). As part of the MAPK/ERK signaling pathway, acts as a negative regulator of apoptosis in cardiomyocytes via promotion of STUB1/CHIP-mediated ubiquitination and degradation of ICER-type isoforms of CREM (By similarity).

Additional Targets

IGF2

Alternative names

Recommended products

Anti-IGF1+IGF2 antibody (ab9572) is a rabbit polyclonal antibody detecting IGF1 in Western Blot, IHC-P, ICC/IF, ELISA, Neut. Suitable for Human, Mouse,.



- Over 110 publications

- Trusted since 2002


Key facts

Isotype
IgG
Form
Lyophilized
Clonality
Polyclonal
Immunogen
  • Recombinant Full Length Protein corresponding to Human IGF1. Database link P05019
Purification technique
Affinity purification Immunogen
Light chain type
unknown
Reconstitution
Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot

Notes

Product Specifications

Anti-IGF1+IGF2 antibody (ab9572) is a rabbit Polyclonal antibody and is validated for use in ICC/IF, IHC-FoFr, IHC-P, Neut, WB, sELISA in human, mouse samples.
Anti-IGF1+IGF2 antibody (ab9572) specifically detects IGF1 + IFG2 (UniProt ID: P05019; Molecular weight: 8kDa) and is sold in 100 µg selling sizes.

Quality and Validation

Abcam's high quality validation processes ensure Anti-IGF1+IGF2 antibody (ab9572) has high sensitivity and specificity.
Anti-IGF1+IGF2 antibody (ab9572) has been cited over 114 times in peer reviewed journals and is trusted by the scientific community.

Although some customers have been successful in IHC we no longer batch test in this application. For an IHC validated antibody please see Anti-IGF1 Receptor antibody [EPR23027-204] ab263903
Anti-IGF1+IGF2 antibody (ab9572) has 8 independent reviews from customers.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGF1+IGF2 antibody (ab9572), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGF1+IGF2 antibody (ab9572)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pancreas tissue labelling IGF1 with ab9572 at 1 μg/mL (45 minute incubation at room temperature). Heat mediated antigen retrieval was performed using a buffer at pH 6. An HRP-labeled polymer detection system was used with a DAB chromogen.

  • Sandwich ELISA - Anti-IGF1+IGF2 antibody (ab9572), expandable thumbnail

    Sandwich ELISA - Anti-IGF1+IGF2 antibody (ab9572)

    To detect hIGF-I by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IGF-I as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-I.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGF1+IGF2 antibody (ab9572), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGF1+IGF2 antibody (ab9572)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling IGF1 with ab9572 at 1 μg/mL (45 minute incubation at room temperature). Heat mediated antigen retrieval was performed using a buffer at pH 6. An HRP-labeled polymer detection system was used with a DAB chromogen.

  • Western blot - Anti-IGF1+IGF2 antibody (ab9572), expandable thumbnail

    Western blot - Anti-IGF1+IGF2 antibody (ab9572)

    All lanes: Western blot - Anti-IGF1+IGF2 antibody (ab9572) at 1/1000 dilution

    Lanes 1 and 3: Mouse IGF-2 recombinant protein (aa25-91, without tag)

    Lanes 2 and 4: Mouse IGF-1 recombinant protein (aa25-91, without tag)

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 22 kDa

    Exposure time: 59s

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com