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AB177467

Anti-IGF2 antibody [EPR12221]

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(8 Publications)

Rabbit Recombinant Monoclonal IGF2 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 8 publications.

View Alternative Names

IGF-2, PP1446, IGF2, Insulin-like growth factor 2, Insulin-like growth factor II, Somatomedin-A, T3M-11-derived growth factor, IGF-II

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-IGF2 antibody [EPR12221] (AB177467)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-IGF2 antibody [EPR12221] (AB177467)

Immunofluorescence analysis of HepG2 cells labeling IGF2 (green) with ab177467 at 1/50 dilution. DAPI nuclear staining (blue).

Western blot - Anti-IGF2 antibody [EPR12221] (AB177467)
  • WB

Supplier Data

Western blot - Anti-IGF2 antibody [EPR12221] (AB177467)

All lanes:

Western blot - Anti-IGF2 antibody [EPR12221] (ab177467) at 1/1000 dilution

All lanes:

Human serum lysate at 10 µg

Secondary

All lanes:

Goat-anti-rabbit HRP at 1/2000 dilution

Predicted band size: 20 kDa

true

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12221

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

ab177467 has been found not to cross react with mouse or rat IGF-2 in western blot testing.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The insulin-like growth factors possess growth-promoting activity (By similarity). Major fetal growth hormone in mammals. Plays a key role in regulating fetoplacental development. IGF2 is influenced by placental lactogen. Also involved in tissue differentiation. In adults, involved in glucose metabolism in adipose tissue, skeletal muscle and liver (Probable). Acts as a ligand for integrin which is required for IGF2 signaling (PubMed : 28873464). Positively regulates myogenic transcription factor MYOD1 function by facilitating the recruitment of transcriptional coactivators, thereby controlling muscle terminal differentiation (By similarity). Inhibits myoblast differentiation and modulates metabolism via increasing the mitochondrial respiration rate (By similarity).. Preptin undergoes glucose-mediated co-secretion with insulin, and acts as a physiological amplifier of glucose-mediated insulin secretion. Exhibits osteogenic properties by increasing osteoblast mitogenic activity through phosphoactivation of MAPK1 and MAPK3.
See full target information IGF2
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

World journal of gastrointestinal oncology 16:3585-3599 PubMed39171181

2024

Insulin-like growth factor 2 targets IGF1R signaling transduction to facilitate metastasis and imatinib resistance in gastrointestinal stromal tumors.

Applications

Unspecified application

Species

Unspecified reactive species

De-Gang Li,Jia-Peng Jiang,Fan-Ye Chen,Wei Wu,Jun Fu,Gong-He Wang,Yu-Bo Li

Heliyon 10:e29682 PubMed38707418

2024

NEK2 promotes the migration, invasion, proliferation of ESCC and mediates ESCC immunotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Shaorui Gu,YakuFujiang Yasen,Mengying Wang,Baiqing Huang,Yongxin Zhou,Wenli Wang

Open medicine (Warsaw, Poland) 18:20230751 PubMed37693837

2023

Circ-UBR4 regulates the proliferation, migration, inflammation, and apoptosis in ox-LDL-induced vascular smooth muscle cells via miR-515-5p/IGF2 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Liuliu Feng,Tianhua Liu,Jun Shi,Yu Wang,Yuya Yang,Wenyin Xiao,Yanyan Bai

Frontiers in oncology 12:922465 PubMed36568212

2022

Exosomes from cisplatin-induced dormant cancer cells facilitate the formation of premetastatic niche in bone marrow through activating glycolysis of BMSCs.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaqi Xu,Xiang Feng,Na Yin,Lujuan Wang,Yaohuan Xie,Yawen Gao,Juanjuan Xiang

Journal of gynecologic oncology 33:e75 PubMed36245227

2022

SP1-induced lncRNA MCF2L-AS1 promotes cisplatin resistance in ovarian cancer by regulating IGF2BP1/IGF2/MEK/ERK axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Zhu,Lijuan Yang,Jianqing Wang,Yan Li,Youguo Chen

Acta biochimica Polonica 69:349-355 PubMed35617351

2022

Krüppel-like factor 4 promotes the proliferation and osteogenic differentiation of BMSCs through SOX2/IGF2 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zhengguo Fei,Chunguang Sun,Xiaoqiang Peng,Mu Han,Qijia Zhou

Cell cycle (Georgetown, Tex.) 20:1561-1577 PubMed34313533

2021

LncRNA SNHG12 regulates ox-LDL-induced endothelial cell injury by the miR-218-5p/IGF2 axis in atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Ping Mao,Xiaowei Liu,Yingzheng Wen,Lijiang Tang,Yimin Tang

European review for medical and pharmacological sciences 21:1803-1809 PubMed28485799

2017

Decreased microRNA-146a in CD4+T cells promote ocular inflammation in thyroid-associated ophthalmopathy by targeting NUMB.

Applications

Unspecified application

Species

Unspecified reactive species

Z-J Hu,J-F He,K-J Li,J Chen,X-R Xie
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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