Rabbit Recombinant Monoclonal IGF2 antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human samples.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-IGF2 antibody [EPR12221] - BSA and Azide free (AB249973), expandable thumbnail](https://content.abcam.com/products/images/igf2-antibody-epr12221-bsa-and-azide-free-ab249973--immunocytochemistry-immunofluorescence-img114270.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-IGF2 antibody [EPR12221] - BSA and Azide free (AB249973)")
![Western blot - Anti-IGF2 antibody [EPR12221] - BSA and Azide free (AB249973), expandable thumbnail](https://content.abcam.com/products/images/igf2-antibody-epr12221-bsa-and-azide-free-ab249973--western-blot-img90478.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-IGF2 antibody [EPR12221] - BSA and Azide free (AB249973)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | WB | IHC-P | |
|---|---|---|---|---|
| Human | Tested | Not recommended | Tested | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human, Mouse | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
Target data
Function
The insulin-like growth factors possess growth-promoting activity (By similarity). Major fetal growth hormone in mammals. Plays a key role in regulating fetoplacental development. IGF2 is influenced by placental lactogen. Also involved in tissue differentiation. In adults, involved in glucose metabolism in adipose tissue, skeletal muscle and liver (Probable). Acts as a ligand for integrin which is required for IGF2 signaling (PubMed:28873464). Positively regulates myogenic transcription factor MYOD1 function by facilitating the recruitment of transcriptional coactivators, thereby controlling muscle terminal differentiation (By similarity). Inhibits myoblast differentiation and modulates metabolism via increasing the mitochondrial respiration rate (By similarity). Preptin undergoes glucose-mediated co-secretion with insulin, and acts as a physiological amplifier of glucose-mediated insulin secretion. Exhibits osteogenic properties by increasing osteoblast mitogenic activity through phosphoactivation of MAPK1 and MAPK3.
Alternative names
IGF-2, PP1446, IGF2, Insulin-like growth factor 2, Insulin-like growth factor II, Somatomedin-A, T3M-11-derived growth factor, IGF-II
Recommended products
Rabbit Recombinant Monoclonal IGF2 antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR12221
- Purification technique
- Affinity purification Protein A
- Specificity
This antibody has been found not to cross react with mouse or rat IGF-2 in western blot testing.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab249973 is the carrier-free version of Anti-IGF2 antibody [EPR12221] ab177467.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Immunocytochemistry/ Immunofluorescence - Anti-IGF2 antibody [EPR12221] - BSA and Azide free (ab249973), expandable thumbnail](https://content.abcam.com/products/images/igf2-antibody-epr12221-bsa-and-azide-free-ab249973--immunocytochemistry-immunofluorescence-img114270.jpg "Immunocytochemistry/ Immunofluorescence - Anti-IGF2 antibody [EPR12221] - BSA and Azide free (ab249973)")
Immunocytochemistry/ Immunofluorescence - Anti-IGF2 antibody [EPR12221] - BSA and Azide free (ab249973)
This data was developed using Anti-IGF2 antibody [EPR12221] ab177467, the same antibody clone in a different buffer formulation.Immunofluorescence analysis of HepG2 cells labeling IGF2 (green) with Anti-IGF2 antibody [EPR12221] ab177467 at 1/50 dilution. DAPI nuclear staining (blue).
![Western blot - Anti-IGF2 antibody [EPR12221] - BSA and Azide free (ab249973), expandable thumbnail](https://content.abcam.com/products/images/igf2-antibody-epr12221-bsa-and-azide-free-ab249973--western-blot-img90478.jpg "Western blot - Anti-IGF2 antibody [EPR12221] - BSA and Azide free (ab249973)")
Western blot - Anti-IGF2 antibody [EPR12221] - BSA and Azide free (ab249973)
This data was developed using Anti-IGF2 antibody [EPR12221] ab177467, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-IGF2 antibody [EPR12221] (Anti-IGF2 antibody [EPR12221] ab177467) at 1/1000 dilution
All lanes: Human serum lysate at 10 µg
SecondaryAll lanes: Goat-anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 20 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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