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Rabbit Monoclonal IGF2 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Human IGF2.

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Images

Western blot - Anti-IGF2 antibody [HL1979] (AB308213), expandable thumbnail
  • Western blot - Anti-IGF2 antibody [HL1979] (AB308213), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Fragment Protein within Human IGF2. The exact immunogen used to generate this antibody is proprietary information. Database link P01344

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested
Mouse
Tested

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Target data

Function

The insulin-like growth factors possess growth-promoting activity (By similarity). Major fetal growth hormone in mammals. Plays a key role in regulating fetoplacental development. IGF2 is influenced by placental lactogen. Also involved in tissue differentiation. In adults, involved in glucose metabolism in adipose tissue, skeletal muscle and liver (Probable). Acts as a ligand for integrin which is required for IGF2 signaling (PubMed:28873464). Positively regulates myogenic transcription factor MYOD1 function by facilitating the recruitment of transcriptional coactivators, thereby controlling muscle terminal differentiation (By similarity). Inhibits myoblast differentiation and modulates metabolism via increasing the mitochondrial respiration rate (By similarity). Preptin undergoes glucose-mediated co-secretion with insulin, and acts as a physiological amplifier of glucose-mediated insulin secretion. Exhibits osteogenic properties by increasing osteoblast mitogenic activity through phosphoactivation of MAPK1 and MAPK3.

Alternative names

IGF-2, PP1446, IGF2, Insulin-like growth factor 2, Insulin-like growth factor II, Somatomedin-A, T3M-11-derived growth factor, IGF-II

Recommended products

Rabbit Monoclonal IGF2 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Human IGF2.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Recombinant Fragment Protein within Human IGF2. The exact immunogen used to generate this antibody is proprietary information. Database link P01344
Carrier free
Yes
Clone number
HL1979
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Anti-IGF2 antibody [HL1979] (ab308213), expandable thumbnail

    Western blot - Anti-IGF2 antibody [HL1979] (ab308213)

    HepG2 whole cell extract and conditioned medium (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IGF2 antibody [HL1979] (ab308213) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

    Lane 1: HepG2 whole cell extract at 30 µg

    Lane 2: HepG2 whole cell extract and conditioned medium at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

  • Western blot - Anti-IGF2 antibody [HL1979] (ab308213), expandable thumbnail

    Western blot - Anti-IGF2 antibody [HL1979] (ab308213)

    Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with IGF2 antibody [HL1979] (ab308213) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

    All lanes: Mouse tissue lysate at 50 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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