Rabbit Recombinant Monoclonal IGF2BP2/IMP-2 antibody. Suitable for IHC-P, WB and reacts with Human, Recombinant full length protein - Human samples. Cited in 7 publications.
Images
![Western blot - Anti-IGF2BP1 + IGF2BP2 antibody [EPR6740(2)] (AB129071), expandable thumbnail](https://content.abcam.com/products/images/igf2bp1-igf2bp2-antibody-epr67402-ab129071--western-blot-img61674.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-IGF2BP1 + IGF2BP2 antibody [EPR6740(2)] (AB129071)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGF2BP1 + IGF2BP2 antibody [EPR6740(2)] (AB129071), expandable thumbnail](https://content.abcam.com/products/images/igf2bp1-igf2bp2-antibody-epr67402-ab129071--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img138482.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGF2BP1 + IGF2BP2 antibody [EPR6740(2)] (AB129071)")
![OI-RD Scanning - Anti-IGF2BP1 + IGF2BP2 antibody [EPR6740(2)] (AB129071), expandable thumbnail](https://content.abcam.com/products/images/igf2bp1-igf2bp2-antibody-epr67402-ab129071--oi-rd-scanning-img95204.jpg/_jcr_content/renditions/webp-475.webp "OI-RD Scanning - Anti-IGF2BP1 + IGF2BP2 antibody [EPR6740(2)] (AB129071)")
![Western blot - Anti-IGF2BP1 + IGF2BP2 antibody [EPR6740(2)] (AB129071), expandable thumbnail](https://content.abcam.com/products/images/igf2bp1-igf2bp2-antibody-epr67402-ab129071--western-blot-img433863.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-IGF2BP1 + IGF2BP2 antibody [EPR6740(2)] (AB129071)")
Publications
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- Heliyon 9:e206592023Comprehensive analysis of the expression of the IGF2BPs gene family in head and neck squamous cell carcinoma: Association with prognostic value and tumor immunity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHai Tang,Jianjiang Zhao,Jingpeng LiuPubMed 37842569
- Journal of cellular biochemistry 124:239-2532022IGF2BP2 enhances LincRNA01116 stability via m A: A potential biomarker and therapeutic target for patients with pre-eclampsia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTingting Zhang,Xiaotong Tang,Yuanyuan Zhu,Cong Wang,Ziyan Jiang,Nana Yang,Tianjun Wang,Lijun Shu,Yetao Xu,Lizhou SunPubMed 36538649
- ESC heart failure 9:2955-29662022HOXA cluster antisense RNA 2 elevates KIAA1522 expression through microRNA-520d-3p and insulin like growth factor 2 mRNA binding protein 3 to promote the growth of vascular smooth muscle cells in thoracic aortic aneurysm.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMinghui Ou,Yaonan Chu,Qian Zhang,Huidong Zhao,Qiang SongPubMed 35730141
- Journal of ovarian research 14:1542021RNA-binding protein IGF2BP2 enhances circ_0000745 abundancy and promotes aggressiveness and stemness of ovarian cancer cells via the microRNA-3187-3p/ERBB4/PI3K/AKT axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShengtan Wang,Zaihong Li,Genhai Zhu,Lan Hong,Chunyan Hu,Kang Wang,Kaiying Cui,Chunbo HaoPubMed 34774079
- Bioengineered 12:11267-112762021Insulin-like growth factor 2 mRNA binding protein 2 regulates proliferation, migration, and angiogenesis of keratinocytes by modulating heparanase stability.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShaomin Zhi,Jun Li,Xiao Kong,Xuemei Xie,Qiangli Zhang,Guoxiang FangPubMed 34753397
- Molecular medicine (Cambridge, Mass.) 27:912021Human umbilical cord mesenchymal stem cells deliver exogenous miR-26a-5p via exosomes to inhibit nucleus pulposus cell pyroptosis through METTL14/NLRP3.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoqiu Yuan,Tiefeng Li,Lei Shi,Jinhao Miao,Yongfei Guo,Yu ChenPubMed 34412584
- Breast cancer research and treatment 172:577-5862018CCN6 regulates IGF2BP2 and HMGA2 signaling in metaplastic carcinomas of the breast.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEmily R McMullen et. al.PubMed 30220054
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | Flow Cyt | WB | ICC/IF | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Tested | Not recommended |
| Mouse | Predicted | Not recommended | Not recommended | Predicted | Not recommended |
| Rat | Predicted | Not recommended | Not recommended | Predicted | Not recommended |
| Recombinant full length protein - Human | Not recommended | Not recommended | Not recommended | Tested | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 - 1/100 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Recombinant full length protein - Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Recombinant full length protein - Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
Species Recombinant full length protein - Human | Dilution info 1/1000 - 1/10000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Recombinant full length protein - Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Function
RNA-binding factor that recruits target transcripts to cytoplasmic protein-RNA complexes (mRNPs). This transcript 'caging' into mRNPs allows mRNA transport and transient storage. It also modulates the rate and location at which target transcripts encounter the translational apparatus and shields them from endonuclease attacks or microRNA-mediated degradation (By similarity). Preferentially binds to N6-methyladenosine (m6A)-containing mRNAs and increases their stability (PubMed:29476152). Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs (PubMed:9891060). Binding is isoform-specific. Binds to beta-actin/ACTB and MYC transcripts. Increases MYC mRNA stability by binding to the coding region instability determinant (CRD) and binding is enhanced by m6A-modification of the CRD (PubMed:29476152).
Additional Targets
IGF2BP1
Alternative names
IMP2, VICKZ2, IGF2BP2, Insulin-like growth factor 2 mRNA-binding protein 2, IGF2 mRNA-binding protein 2, IMP-2, Hepatocellular carcinoma autoantigen p62, IGF-II mRNA-binding protein 2, VICKZ family member 2
Recommended products
Rabbit Recombinant Monoclonal IGF2BP2/IMP-2 antibody. Suitable for IHC-P, WB and reacts with Human, Recombinant full length protein - Human samples. Cited in 7 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR6740(2)
- Purification technique
- Affinity purification Protein A
- Dissociation constant
- 1 x 10-10 M
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Stable for 12 months at -20°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-IGF2BP1 + IGF2BP2 antibody [EPR6740(2)] (ab129071), expandable thumbnail](https://content.abcam.com/products/images/igf2bp1-igf2bp2-antibody-epr67402-ab129071--western-blot-img61674.jpg "Western blot - Anti-IGF2BP1 + IGF2BP2 antibody [EPR6740(2)] (ab129071)")
Western blot - Anti-IGF2BP1 + IGF2BP2 antibody [EPR6740(2)] (ab129071)
All lanes: Western blot - Anti-IGF2BP1 + IGF2BP2 antibody [EPR6740(2)] (ab129071) at 1/1000 dilution
Lane 1: 293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg
Lane 2: Caco-2 cell lysate at 10 µg
SecondaryAll lanes: Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 10 kDa, 33 kDa, 38 kDa, 41 kDa, 43 kDa, 66 kDa, 78 kDa
Observed band size: 10 kDa, 38 kDa, 78 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGF2BP1 + IGF2BP2 antibody [EPR6740(2)] (ab129071), expandable thumbnail](https://content.abcam.com/products/images/igf2bp1-igf2bp2-antibody-epr67402-ab129071--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img138482.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGF2BP1 + IGF2BP2 antibody [EPR6740(2)] (ab129071)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGF2BP1 + IGF2BP2 antibody [EPR6740(2)] (ab129071)
ab129071, at 1/50 dilution, staining IGF2BP1 + IGF2BP2 in paraffin-embedded Human colon tissue by Immunohistochemistry
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![OI-RD Scanning - Anti-IGF2BP1 + IGF2BP2 antibody [EPR6740(2)] (ab129071), expandable thumbnail](https://content.abcam.com/products/images/igf2bp1-igf2bp2-antibody-epr67402-ab129071--oi-rd-scanning-img95204.jpg "OI-RD Scanning - Anti-IGF2BP1 + IGF2BP2 antibody [EPR6740(2)] (ab129071)")
OI-RD Scanning - Anti-IGF2BP1 + IGF2BP2 antibody [EPR6740(2)] (ab129071)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.![Western blot - Anti-IGF2BP1 + IGF2BP2 antibody [EPR6740(2)] (ab129071), expandable thumbnail](https://content.abcam.com/products/images/igf2bp1-igf2bp2-antibody-epr67402-ab129071--western-blot-img433863.jpg "Western blot - Anti-IGF2BP1 + IGF2BP2 antibody [EPR6740(2)] (ab129071)")
Western blot - Anti-IGF2BP1 + IGF2BP2 antibody [EPR6740(2)] (ab129071)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
All lanes: Western blot - Anti-IGF2BP1 + IGF2BP2 antibody [EPR6740(2)] (ab129071) at 1/1000 dilution
Lane 1: C-Myc/DDK -tagged full length Human IGF2BP1 recombinant protein at 10 ng with 5% NFDM/TBST
Lane 2: C-Myc/DDK -tagged full length Human IGF2BP2 recombinant protein at 10 ng with 5% NFDM/TBST
Lane 3: N-His -tagged full length Human IGF2BP3 recombinant protein at 10 ng with 5% NFDM/TBST
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 75 kDa
Exposure time: 180s
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