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AB248279

Anti-IGF2BP1 + IGF2BP2 antibody [EPR6740(2)] - BSA and Azide free

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(2 Publications)

Rabbit Recombinant Monoclonal IGF2BP2/IMP-2 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Recombinant full length protein - Human samples. Cited in 2 publications.

View Alternative Names

IMP2, VICKZ2, IGF2BP2, Insulin-like growth factor 2 mRNA-binding protein 2, IGF2 mRNA-binding protein 2, IMP-2, Hepatocellular carcinoma autoantigen p62, IGF-II mRNA-binding protein 2, VICKZ family member 2

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGF2BP1 + IGF2BP2 antibody [EPR6740(2)] - BSA and Azide free (AB248279)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGF2BP1 + IGF2BP2 antibody [EPR6740(2)] - BSA and Azide free (AB248279)

This data was developed using ab129071, the same antibody clone in a different buffer formulation.

ab129071, at 1/50 dilution, staining IGF2BP1 + IGF2BP2 in paraffin-embedded Human colon tissue by Immunohistochemistry

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Western blot - Anti-IGF2BP1 + IGF2BP2 antibody [EPR6740(2)] - BSA and Azide free (AB248279)
  • WB

Unknown

Western blot - Anti-IGF2BP1 + IGF2BP2 antibody [EPR6740(2)] - BSA and Azide free (AB248279)

This data was developed using ab129071, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-IGF2BP1 + IGF2BP2 antibody [EPR6740(2)] (<a href='/en-us/products/primary-antibodies/igf2bp1-igf2bp2-antibody-epr67402-ab129071'>ab129071</a>) at 1/1000 dilution

Lane 1:

293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

Lane 2:

Caco-2 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 10 kDa,33 kDa,38 kDa,41 kDa,43 kDa,66 kDa,78 kDa

Observed band size: 10 kDa,38 kDa,78 kDa

false

Western blot - Anti-IGF2BP1 + IGF2BP2 antibody [EPR6740(2)] - BSA and Azide free (AB248279)
  • WB

Lab

Western blot - Anti-IGF2BP1 + IGF2BP2 antibody [EPR6740(2)] - BSA and Azide free (AB248279)

This data was developed using ab129071, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-IGF2BP1 + IGF2BP2 antibody [EPR6740(2)] (<a href='/en-us/products/primary-antibodies/igf2bp1-igf2bp2-antibody-epr67402-ab129071'>ab129071</a>) at 1/1000 dilution

Lane 1:

C-Myc/DDK -tagged full length Human IGF2BP1 recombinant protein at 10 ng

Lane 2:

C-Myc/DDK -tagged full length Human IGF2BP2 recombinant protein at 10 ng

Lane 3:

N-His -tagged full length Human IGF2BP3 recombinant protein at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 75 kDa

false

Exposure time: 180s

OI-RD Scanning - Anti-IGF2BP1 + IGF2BP2 antibody [EPR6740(2)] - BSA and Azide free (AB248279)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-IGF2BP1 + IGF2BP2 antibody [EPR6740(2)] - BSA and Azide free (AB248279)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6740(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Recombinant full length protein - Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

ab248279 is the carrier-free version of ab129071.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

RNA-binding factor that recruits target transcripts to cytoplasmic protein-RNA complexes (mRNPs). This transcript 'caging' into mRNPs allows mRNA transport and transient storage. It also modulates the rate and location at which target transcripts encounter the translational apparatus and shields them from endonuclease attacks or microRNA-mediated degradation (By similarity). Preferentially binds to N6-methyladenosine (m6A)-containing mRNAs and increases their stability (PubMed : 29476152). Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs (PubMed : 9891060). Binding is isoform-specific. Binds to beta-actin/ACTB and MYC transcripts. Increases MYC mRNA stability by binding to the coding region instability determinant (CRD) and binding is enhanced by m6A-modification of the CRD (PubMed : 29476152).
See full target information IGF2BP2

Additional targets

IGF2BP1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell proliferation 56:e13400 PubMed36642844

2023

Non-SMC condensin I complex subunit H participates in anti-programmed cell death-1 resistance of clear cell renal cell carcinomas.

Applications

Unspecified application

Species

Unspecified reactive species

Zhi Chen,Weiqiang Ruan,Chunhao Guo,Ke Chen,Le Li,Jihua Tian,Zhiquan Hu,Dan Peng,Xing Zeng

PeerJ 10:e13735 PubMed35891648

2022

Identification and validation of immune related core transcription factors in NAFLD.

Applications

Unspecified application

Species

Unspecified reactive species

Minbo Zhang,Yu Zhang,Xiaoxiao Jiao,Linying Lai,Yiting Qian,Bo Sun,Wenzhuo Yang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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