Anti-IGF2BP2/IMP-2 antibody [1E3.01E5]

• WB

Supplier Data

Western blot - Anti-IGF2BP2/IMP-2 antibody [1E3.01E5] (AB128175)

All lanes:

Western blot - Anti-IGF2BP2/IMP-2 antibody [1E3.01E5] (ab128175) at 1/1000 dilution

Lane 1:

Hep-G2 whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 50 µg

Lane 3:

HEK293T whole cell lysate at 50 µg

Lane 4:

Jurkat whole cell lysate at 50 µg

Lane 5:

NIH 3T3 whole cell lysate at 50 µg

Secondary

All lanes:

Western blot - Goat Anti-Mouse IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-hrp-ab97023'>ab97023</a>)

Predicted band size: 66 kDa,70 kDa

Observed band size: 70 kDa,66 kDa

true

Exposure time: 10s

• IP

Supplier Data

Immunoprecipitation - Anti-IGF2BP2/IMP-2 antibody [1E3.01E5] (AB128175)

Lanes 1 + 2 : Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK-293T cells prepared using NETN lysis buffer.

Lane 1 : ab128175
Lane 2 : Control IgG

ab128175 was used for IP at 20 μl/mg lysate. For blotting immunoprecipitated IGF2BP2, ab128175 was used at 1 : 1000. An HRP-conjugated goat anti-mouse IgG secondary antibody was used. Chemiluminescence with an exposure time of 3 seconds.

All lanes:

Immunoprecipitation - Anti-IGF2BP2/IMP-2 antibody [1E3.01E5] (ab128175) at 1/1000 dilution

All lanes:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

Predicted band size: 66 kDa

false

Exposure time: 3s

• WB

Supplier Data

Western blot - Anti-IGF2BP2/IMP-2 antibody [1E3.01E5] (AB128175)

Western blot analysis of whole cell lysates prepared using NETN lysis buffer labeling IGF2BP2/IMP-2 with ab128175 at a dilution of 1/1000. An HRP-conjugated goat anti-mouse IgG secondary was used. Chemiluminescence with an exposure time of 3 seconds.

All lanes:

Western blot - Anti-IGF2BP2/IMP-2 antibody [1E3.01E5] (ab128175) at 1/1000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 50 µg

Lane 2:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 4:

TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate at 50 µg

Lane 5:

NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 50 µg

Predicted band size: 66 kDa

false

Exposure time: 3s