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AB250280

Anti-IGFBP1 antibody [EPR14471(B)] - BSA and Azide free

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Rabbit Recombinant Monoclonal IGFBP1 antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

IBP1, IGFBP1, Insulin-like growth factor-binding protein 1, IBP-1, IGF-binding protein 1, IGFBP-1, Placental protein 12, PP12

2 Images
Western blot - Anti-IGFBP1 antibody [EPR14471(B)] - BSA and Azide free (AB250280)
  • WB

Supplier Data

Western blot - Anti-IGFBP1 antibody [EPR14471(B)] - BSA and Azide free (AB250280)

This data was developed using ab180948, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-IGFBP1 antibody [EPR14471(B)] (<a href='/en-us/products/primary-antibodies/igfbp1-antibody-epr14471b-ab180948'>ab180948</a>) at 1/20000 dilution

All lanes:

HepG2 cell lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 28 kDa

Observed band size: 28 kDa

false

Western blot - Anti-IGFBP1 antibody [EPR14471(B)] - BSA and Azide free (AB250280)
  • WB

Supplier Data

Western blot - Anti-IGFBP1 antibody [EPR14471(B)] - BSA and Azide free (AB250280)

This data was developed using ab180948, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-IGFBP1 antibody [EPR14471(B)] (<a href='/en-us/products/primary-antibodies/igfbp1-antibody-epr14471b-ab180948'>ab180948</a>) at 1/5000 dilution

Lane 1:

Human fetal liver lysate at 20 µg

Lane 2:

Human placenta lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 28 kDa

Observed band size: 28 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14471(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab250280 is the carrier-free version of ab180948.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Multifunctional protein that plays a critical role in regulating the availability of IGFs such as IGF1 and IGF2 to their receptors and thereby regulates IGF-mediated cellular processes including cell migration, proliferation, differentiation or apoptosis in a cell-type specific manner (PubMed : 11397844, PubMed : 15972819). Plays also a positive role in cell migration by interacting with integrin ITGA5 : ITGB1 through its RGD motif (PubMed : 7504269). Mechanistically, binding to integrins leads to activation of focal adhesion kinase/PTK2 and stimulation of the mitogen-activated protein kinase (MAPK) pathway (PubMed : 11397844). Regulates cardiomyocyte apoptosis by suppressing HIF-1alpha/HIF1A ubiquitination and subsequent degradation (By similarity).
See full target information IGFBP1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com