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Rabbit Recombinant Monoclonal IGFBP2 antibody. Suitable for IP, WB, IHC-Fr, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 10 publications.


Images

Western blot - Anti-IGFBP2 antibody [EPR18012-257] (AB188200), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGFBP2 antibody [EPR18012-257] (AB188200), expandable thumbnail
  • Immunoprecipitation - Anti-IGFBP2 antibody [EPR18012-257] (AB188200), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-IGFBP2 antibody [EPR18012-257] (AB188200), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-IGFBP2 antibody [EPR18012-257] (AB188200), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-FrFlow Cyt (Intra)IHC-P
Human
Tested
Tested
Expected
Tested
Expected
Mouse
Expected
Expected
Tested
Tested
Tested
Rat
Expected
Expected
Tested
Expected
Expected

Tested
Tested

Species

Human

Dilution info

1/30

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/500

Notes

-

Species

Rat

Dilution info

1/500

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/60

Notes

-

Species

Human

Dilution info

1/60

Notes

-

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

This antibody is not suitable for human and rat species in IHC application due to non-specific or negative staining.

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species

Human, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Associated Products

Select an associated product type

4 products for Alternative Product

Target data

Function

Multifunctional protein that plays a critical role in regulating the availability of IGFs such as IGF1 and IGF2 to their receptors and thereby regulates IGF-mediated cellular processes including proliferation, differentiation, and apoptosis in a cell-type specific manner (PubMed:18563800, PubMed:38796567). Functions coordinately with receptor protein tyrosine phosphatase beta/PTPRB and the IGF1 receptor to regulate IGF1-mediated signaling by stimulating the phosphorylation of PTEN leading to its inactivation and AKT1 activation (PubMed:22869525). Plays a positive role in cell migration via interaction with integrin alpha5/ITGA5 through an RGD motif (PubMed:16569642). Additionally, interaction with ITGA5/ITGB1 enhances the adhesion of endothelial progenitor cells to endothelial cells (PubMed:26076738). Upon mitochondrial damage, facilitates apoptosis with ITGA5 of podocytes, and then activates the phosphorylation of focal adhesion kinase (FAK)-mediated mitochondrial injury (PubMed:38796567).

Alternative names

Recommended products

Rabbit Recombinant Monoclonal IGFBP2 antibody. Suitable for IP, WB, IHC-Fr, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 10 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR18012-257

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

  • Western blot - Anti-IGFBP2 antibody [EPR18012-257] (ab188200), expandable thumbnail

    Western blot - Anti-IGFBP2 antibody [EPR18012-257] (ab188200)

    Blocking: 5% NFDM/TBST.

    The band in lane 1 is human IgG heavy chain which is often observed in serum and plasma samples.

    All lanes: Western blot - Anti-IGFBP2 antibody [EPR18012-257] (ab188200) at 1/1000 dilution

    Lane 1: Human serum at 20 µg

    Lane 2: Human liver lysate at 20 µg

    Secondary

    All lanes: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 35 kDa

    Observed band size: 33 kDa

    Exposure time: 3min

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGFBP2 antibody [EPR18012-257] (ab188200), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGFBP2 antibody [EPR18012-257] (ab188200)

    Immunohistochemical analysis of paraffin-embedded mouse choroid plexus tissue labeling IGFBP2 with ab188200 at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining on mouse choroid plexus (PMID: 7525264; PMID: 7678219) is observed. Counter stained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunoprecipitation - Anti-IGFBP2 antibody [EPR18012-257] (ab188200), expandable thumbnail

    Immunoprecipitation - Anti-IGFBP2 antibody [EPR18012-257] (ab188200)

    IGFBP2 was immunoprecipitated from 0.35 mg of human serum with ab188200 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab188200 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10000 dilution.

    Lane 1: Human serum 10 μg (Input).
    Lane 2: ab188200 IP in Human serum (+).
    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab188200 in human serum (-).

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

    The band in lane 1 is human IgG heavy chain which is often observed in serum and plasma samples.

    All lanes: Immunoprecipitation - Anti-IGFBP2 antibody [EPR18012-257] (ab188200)

    Predicted band size: 35 kDa

    Exposure time: 1s

  • Immunohistochemistry (Frozen sections) - Anti-IGFBP2 antibody [EPR18012-257] (ab188200), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-IGFBP2 antibody [EPR18012-257] (ab188200)

    Immunohistochemical analysis of 4% PFA fixed, 0.2% TritonX-100 permeabilized mouse brain (choroid plexus) tissue labeling IGFBP2 with ab188200 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution. Cytoplasmic staining in the epithelial cells of choroid plexus on mouse tissue section is observed. Counter stained with DAPI.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.
    Perform heat mediated antigen retrieval using Tris-EDTA (pH 9.0) (ab94681).

  • Flow Cytometry (Intracellular) - Anti-IGFBP2 antibody [EPR18012-257] (ab188200), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-IGFBP2 antibody [EPR18012-257] (ab188200)

    Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized T-47D (human ductal breast epithelial tumor epithelial cell) cell line labeling IGFBP2 with ab188200 at 1/60 (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), at 1/2000 dilution was used as the secondary antibody.

  • Western blot - Anti-IGFBP2 antibody [EPR18012-257] (ab188200), expandable thumbnail

    Western blot - Anti-IGFBP2 antibody [EPR18012-257] (ab188200)

    Blocking: 5% NFDM/TBST.

    The expression profile observed on T-47D is consistent with what has been described in the literature (PMID: 23515291).

    All lanes: Western blot - Anti-IGFBP2 antibody [EPR18012-257] (ab188200) at 1/1000 dilution

    Lane 1: Mouse serum at 20 µg

    Lane 2: T-47D (human ductal breast epithelial tumor epithelial cell) whole cell lysate at 20 µg

    Lane 3: Rat serum at 20 µg

    Lane 4: RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 35 kDa

    Observed band size: 33 kDa

    Exposure time: 3min

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGFBP2 antibody [EPR18012-257] (ab188200), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGFBP2 antibody [EPR18012-257] (ab188200)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling IGFBP2 with ab188200 at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining on mouse liver (PMID: 7678219) is observed. Counter stained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Frozen sections) - Anti-IGFBP2 antibody [EPR18012-257] (ab188200), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-IGFBP2 antibody [EPR18012-257] (ab188200)

    Immunohistochemical analysis of 4% PFA fixed, 0.2% TritonX-100 permeabilized rat brain (choroid plexus) tissue labeling IGFBP2 with ab188200 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution. Cytoplasmic staining in the epithelial cells of choroid plexus on rat tissue section is observed. Counter stained with DAPI.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.
    Perform heat mediated antigen retrieval using Tris-EDTA (pH 9.0) (ab94681).

  • Flow Cytometry (Intracellular) - Anti-IGFBP2 antibody [EPR18012-257] (ab188200), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-IGFBP2 antibody [EPR18012-257] (ab188200)

    Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cell line labeling IGFBP2 with ab188200 at 1/60 (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), at 1/2000 dilution was used as the secondary antibody.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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