Anti-IGFBP2 antibody [EPR18012-257]

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-IGFBP2 antibody [EPR18012-257] (AB188200)

Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized T-47D (human ductal breast epithelial tumor epithelial cell) cell line labeling IGFBP2 with ab188200 at 1/60 (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

• IP

Lab

Immunoprecipitation - Anti-IGFBP2 antibody [EPR18012-257] (AB188200)

IGFBP2 was immunoprecipitated from 0.35 mg of human serum with ab188200 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab188200 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : Human serum 10 μg (Input).
Lane 2 : ab188200 IP in Human serum (+).
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab188200 in human serum (-).

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 1 second.

The band in lane 1 is human IgG heavy chain which is often observed in serum and plasma samples.

All lanes:

Immunoprecipitation - Anti-IGFBP2 antibody [EPR18012-257] (ab188200)

Predicted band size: 35 kDa

false

Exposure time: 1s

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-IGFBP2 antibody [EPR18012-257] (AB188200)

Immunohistochemical analysis of 4% PFA fixed, 0.2% TritonX-100 permeabilized rat brain (choroid plexus) tissue labeling IGFBP2 with ab188200 at 1/500 dilution, followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at 1/1000 dilution. Cytoplasmic staining in the epithelial cells of choroid plexus on rat tissue section is observed. Counter stained with DAPI.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at 1/1000 dilution.
Perform heat mediated antigen retrieval using Tris-EDTA (pH 9.0) (ab94681).

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-IGFBP2 antibody [EPR18012-257] (AB188200)

Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cell line labeling IGFBP2 with ab188200 at 1/60 (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGFBP2 antibody [EPR18012-257] (AB188200)

Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling IGFBP2 with ab188200 at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining on mouse liver (PMID : 7678219) is observed. Counter stained with hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGFBP2 antibody [EPR18012-257] (AB188200)

Immunohistochemical analysis of paraffin-embedded mouse choroid plexus tissue labeling IGFBP2 with ab188200 at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining on mouse choroid plexus (PMID : 7525264; PMID : 7678219) is observed. Counter stained with hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-IGFBP2 antibody [EPR18012-257] (AB188200)

Immunohistochemical analysis of 4% PFA fixed, 0.2% TritonX-100 permeabilized mouse brain (choroid plexus) tissue labeling IGFBP2 with ab188200 at 1/500 dilution, followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at 1/1000 dilution. Cytoplasmic staining in the epithelial cells of choroid plexus on mouse tissue section is observed. Counter stained with DAPI.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at 1/1000 dilution.
Perform heat mediated antigen retrieval using Tris-EDTA (pH 9.0) (ab94681).

• WB

Supplier Data

Western blot - Anti-IGFBP2 antibody [EPR18012-257] (AB188200)

Blocking : 5% NFDM/TBST.

The expression profile observed on T-47D is consistent with what has been described in the literature (PMID : 23515291).

All lanes:

Western blot - Anti-IGFBP2 antibody [EPR18012-257] (ab188200) at 1/1000 dilution

Lane 1:

Mouse serum at 20 µg

Lane 2:

T-47D (human ductal breast epithelial tumor epithelial cell) whole cell lysate at 20 µg

Lane 3:

Rat serum at 20 µg

Lane 4:

RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 35 kDa

Observed band size: 33 kDa

true

Exposure time: 3min

• WB

Supplier Data

Western blot - Anti-IGFBP2 antibody [EPR18012-257] (AB188200)

Blocking : 5% NFDM/TBST.

The band in lane 1 is human IgG heavy chain which is often observed in serum and plasma samples.

All lanes:

Western blot - Anti-IGFBP2 antibody [EPR18012-257] (ab188200) at 1/1000 dilution

Lane 1:

Human serum at 20 µg

Lane 2:

Human liver lysate at 20 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 35 kDa

Observed band size: 33 kDa

true

Exposure time: 3min