Rabbit Recombinant Monoclonal IGFBP2 antibody. Carrier free. Suitable for IP, WB, IHC-Fr, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGFBP2 antibody [EPR18012-257] - BSA and Azide free (AB225763), expandable thumbnail](https://content.abcam.com/products/images/igfbp2-antibody-epr18012-257-bsa-and-azide-free-ab225763--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img132712.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGFBP2 antibody [EPR18012-257] - BSA and Azide free (AB225763)")
![Immunoprecipitation - Anti-IGFBP2 antibody [EPR18012-257] - BSA and Azide free (AB225763), expandable thumbnail](https://content.abcam.com/products/images/igfbp2-antibody-epr18012-257-bsa-and-azide-free-ab225763--immunoprecipitation-img5039.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-IGFBP2 antibody [EPR18012-257] - BSA and Azide free (AB225763)")
![Immunohistochemistry (Frozen sections) - Anti-IGFBP2 antibody [EPR18012-257] - BSA and Azide free (AB225763), expandable thumbnail](https://content.abcam.com/products/images/igfbp2-antibody-epr18012-257-bsa-and-azide-free-ab225763--immunohistochemistry-frozen-sections-img41785.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-IGFBP2 antibody [EPR18012-257] - BSA and Azide free (AB225763)")
![Flow Cytometry (Intracellular) - Anti-IGFBP2 antibody [EPR18012-257] - BSA and Azide free (AB225763), expandable thumbnail](https://content.abcam.com/products/images/igfbp2-antibody-epr18012-257-bsa-and-azide-free-ab225763--flow-cytometry-intracellular-img13750.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-IGFBP2 antibody [EPR18012-257] - BSA and Azide free (AB225763)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGFBP2 antibody [EPR18012-257] - BSA and Azide free (AB225763), expandable thumbnail](https://content.abcam.com/products/images/igfbp2-antibody-epr18012-257-bsa-and-azide-free-ab225763--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img107743.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGFBP2 antibody [EPR18012-257] - BSA and Azide free (AB225763)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-Fr | Flow Cyt (Intra) | IHC-P | |
|---|---|---|---|---|---|
| Human | Tested | Expected | Expected | Tested | Expected |
| Mouse | Expected | Expected | Tested | Expected | Tested |
| Rat | Predicted | Expected | Predicted | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes This antibody is not suitable for human and rat species in IHC application due to non-specific or negative staining. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Function
Inhibits IGF-mediated growth and developmental rates. IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
Alternative names
Insulin-like growth factor-binding protein 2, IBP-2, IGF-binding protein 2, IGFBP-2, BP2, IBP2, IGFBP2
Recommended products
Rabbit Recombinant Monoclonal IGFBP2 antibody. Carrier free. Suitable for IP, WB, IHC-Fr, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR18012-257
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab225763 is the carrier-free version of Anti-IGFBP2 antibody [EPR18012-257] ab188200.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGFBP2 antibody [EPR18012-257] - BSA and Azide free (ab225763), expandable thumbnail](https://content.abcam.com/products/images/igfbp2-antibody-epr18012-257-bsa-and-azide-free-ab225763--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img132712.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGFBP2 antibody [EPR18012-257] - BSA and Azide free (ab225763)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGFBP2 antibody [EPR18012-257] - BSA and Azide free (ab225763)
Immunohistochemical analysis of paraffin-embedded mouse choroid plexus tissue labeling IGFBP2 with Anti-IGFBP2 antibody [EPR18012-257] ab188200 at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining on mouse choroid plexus (PMID: 7525264; PMID: 7678219) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (HRP) ready to use.Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-IGFBP2 antibody [EPR18012-257] ab188200).
![Immunoprecipitation - Anti-IGFBP2 antibody [EPR18012-257] - BSA and Azide free (ab225763), expandable thumbnail](https://content.abcam.com/products/images/igfbp2-antibody-epr18012-257-bsa-and-azide-free-ab225763--immunoprecipitation-img5039.jpg "Immunoprecipitation - Anti-IGFBP2 antibody [EPR18012-257] - BSA and Azide free (ab225763)")
Immunoprecipitation - Anti-IGFBP2 antibody [EPR18012-257] - BSA and Azide free (ab225763)
IGFBP2 was immunoprecipitated from 0.35 mg of human serum with Anti-IGFBP2 antibody [EPR18012-257] ab188200 at 1/30 dilution. Western blot was performed from the immunoprecipitate using Anti-IGFBP2 antibody [EPR18012-257] ab188200 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10000 dilution.
Lane 1: Human serum 10 μg (Input).
Lane 2: Anti-IGFBP2 antibody [EPR18012-257] ab188200 IP in Human serum (+).
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-IGFBP2 antibody [EPR18012-257] ab188200 in human serum (-).Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
The band in lane 1 is human IgG heavy chain which is often observed in serum and plasma samples.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-IGFBP2 antibody [EPR18012-257] ab188200).
All lanes: Immunoprecipitation - Anti-IGFBP2 antibody [EPR18012-257] (Anti-IGFBP2 antibody [EPR18012-257] ab188200)
Predicted band size: 35 kDa
Exposure time: 1s
![Immunohistochemistry (Frozen sections) - Anti-IGFBP2 antibody [EPR18012-257] - BSA and Azide free (ab225763), expandable thumbnail](https://content.abcam.com/products/images/igfbp2-antibody-epr18012-257-bsa-and-azide-free-ab225763--immunohistochemistry-frozen-sections-img41785.jpg "Immunohistochemistry (Frozen sections) - Anti-IGFBP2 antibody [EPR18012-257] - BSA and Azide free (ab225763)")
Immunohistochemistry (Frozen sections) - Anti-IGFBP2 antibody [EPR18012-257] - BSA and Azide free (ab225763)
Immunohistochemical analysis of 4% PFA fixed, 0.2% TritonX-100 permeabilized mouse brain (choroid plexus) tissue labeling IGFBP2 with Anti-IGFBP2 antibody [EPR18012-257] ab188200 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution. Cytoplasmic staining in the epithelial cells of choroid plexus on mouse tissue section is observed. Counter stained with DAPI.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.
Perform heat mediated antigen retrieval using Tris-EDTA (pH 9.0) (ab94681).This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-IGFBP2 antibody [EPR18012-257] ab188200).
![Flow Cytometry (Intracellular) - Anti-IGFBP2 antibody [EPR18012-257] - BSA and Azide free (ab225763), expandable thumbnail](https://content.abcam.com/products/images/igfbp2-antibody-epr18012-257-bsa-and-azide-free-ab225763--flow-cytometry-intracellular-img13750.jpg "Flow Cytometry (Intracellular) - Anti-IGFBP2 antibody [EPR18012-257] - BSA and Azide free (ab225763)")
Flow Cytometry (Intracellular) - Anti-IGFBP2 antibody [EPR18012-257] - BSA and Azide free (ab225763)
Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized T-47D (human ductal breast epithelial tumor epithelial cell) cell line labeling IGFBP2 with Anti-IGFBP2 antibody [EPR18012-257] ab188200 at 1/60 (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), at 1/2000 dilution was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-IGFBP2 antibody [EPR18012-257] ab188200).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGFBP2 antibody [EPR18012-257] - BSA and Azide free (ab225763), expandable thumbnail](https://content.abcam.com/products/images/igfbp2-antibody-epr18012-257-bsa-and-azide-free-ab225763--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img107743.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGFBP2 antibody [EPR18012-257] - BSA and Azide free (ab225763)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGFBP2 antibody [EPR18012-257] - BSA and Azide free (ab225763)
Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling IGFBP2 with Anti-IGFBP2 antibody [EPR18012-257] ab188200 at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining on mouse liver (PMID: 7678219) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (HRP) ready to use.Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-IGFBP2 antibody [EPR18012-257] ab188200).
![Immunohistochemistry (Frozen sections) - Anti-IGFBP2 antibody [EPR18012-257] - BSA and Azide free (ab225763), expandable thumbnail](https://content.abcam.com/products/images/igfbp2-antibody-epr18012-257-bsa-and-azide-free-ab225763--immunohistochemistry-frozen-sections-img3162.jpg "Immunohistochemistry (Frozen sections) - Anti-IGFBP2 antibody [EPR18012-257] - BSA and Azide free (ab225763)")
Immunohistochemistry (Frozen sections) - Anti-IGFBP2 antibody [EPR18012-257] - BSA and Azide free (ab225763)
Immunohistochemical analysis of 4% PFA fixed, 0.2% TritonX-100 permeabilized rat brain (choroid plexus) tissue labeling IGFBP2 with Anti-IGFBP2 antibody [EPR18012-257] ab188200 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution. Cytoplasmic staining in the epithelial cells of choroid plexus on rat tissue section is observed. Counter stained with DAPI.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.
Perform heat mediated antigen retrieval using Tris-EDTA (pH 9.0) (ab94681).This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-IGFBP2 antibody [EPR18012-257] ab188200).
![Flow Cytometry (Intracellular) - Anti-IGFBP2 antibody [EPR18012-257] - BSA and Azide free (ab225763), expandable thumbnail](https://content.abcam.com/products/images/igfbp2-antibody-epr18012-257-bsa-and-azide-free-ab225763--flow-cytometry-intracellular-img415469.jpg "Flow Cytometry (Intracellular) - Anti-IGFBP2 antibody [EPR18012-257] - BSA and Azide free (ab225763)")
Flow Cytometry (Intracellular) - Anti-IGFBP2 antibody [EPR18012-257] - BSA and Azide free (ab225763)
Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cell line labeling IGFBP2 with Anti-IGFBP2 antibody [EPR18012-257] ab188200 at 1/60 (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), at 1/2000 dilution was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-IGFBP2 antibody [EPR18012-257] ab188200).
![Immunohistochemistry (Frozen sections) - Anti-IGFBP2 antibody [EPR18012-257] - BSA and Azide free (ab225763), expandable thumbnail](https://content.abcam.com/products/images/igfbp2-antibody-epr18012-257-bsa-and-azide-free-ab225763--immunohistochemistry-frozen-sections-img3162.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-IGFBP2 antibody [EPR18012-257] - BSA and Azide free (ab225763)")
![Flow Cytometry (Intracellular) - Anti-IGFBP2 antibody [EPR18012-257] - BSA and Azide free (ab225763), expandable thumbnail](https://content.abcam.com/products/images/igfbp2-antibody-epr18012-257-bsa-and-azide-free-ab225763--flow-cytometry-intracellular-img415469.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-IGFBP2 antibody [EPR18012-257] - BSA and Azide free (ab225763)")
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