Anti-IGFBP2 antibody [EPR18012-257] - BSA and Azide free

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-IGFBP2 antibody [EPR18012-257] - BSA and Azide free (AB225763)

Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized T-47D (human ductal breast epithelial tumor epithelial cell) cell line labeling IGFBP2 with ab188200 at 1/60 (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab188200).

• IP

Lab

Immunoprecipitation - Anti-IGFBP2 antibody [EPR18012-257] - BSA and Azide free (AB225763)

IGFBP2 was immunoprecipitated from 0.35 mg of human serum with ab188200 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab188200 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : Human serum 10 μg (Input).
Lane 2 : ab188200 IP in Human serum (+).
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab188200 in human serum (-).

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 1 second.

The band in lane 1 is human IgG heavy chain which is often observed in serum and plasma samples.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab188200).

All lanes:

Immunoprecipitation - Anti-IGFBP2 antibody [EPR18012-257] (<a href='/en-us/products/primary-antibodies/igfbp2-antibody-epr18012-257-ab188200'>ab188200</a>)

Predicted band size: 35 kDa

false

Exposure time: 1s

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-IGFBP2 antibody [EPR18012-257] - BSA and Azide free (AB225763)

Immunohistochemical analysis of 4% PFA fixed, 0.2% TritonX-100 permeabilized mouse brain (choroid plexus) tissue labeling IGFBP2 with ab188200 at 1/500 dilution, followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at 1/1000 dilution. Cytoplasmic staining in the epithelial cells of choroid plexus on mouse tissue section is observed. Counter stained with DAPI.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at 1/1000 dilution.
Perform heat mediated antigen retrieval using Tris-EDTA (pH 9.0) (ab94681).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab188200).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGFBP2 antibody [EPR18012-257] - BSA and Azide free (AB225763)

Immunohistochemical analysis of paraffin-embedded mouse choroid plexus tissue labeling IGFBP2 with ab188200 at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining on mouse choroid plexus (PMID : 7525264; PMID : 7678219) is observed. Counter stained with hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab188200).

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-IGFBP2 antibody [EPR18012-257] - BSA and Azide free (AB225763)

Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cell line labeling IGFBP2 with ab188200 at 1/60 (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab188200).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGFBP2 antibody [EPR18012-257] - BSA and Azide free (AB225763)

Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling IGFBP2 with ab188200 at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining on mouse liver (PMID : 7678219) is observed. Counter stained with hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab188200).

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-IGFBP2 antibody [EPR18012-257] - BSA and Azide free (AB225763)

Immunohistochemical analysis of 4% PFA fixed, 0.2% TritonX-100 permeabilized rat brain (choroid plexus) tissue labeling IGFBP2 with ab188200 at 1/500 dilution, followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at 1/1000 dilution. Cytoplasmic staining in the epithelial cells of choroid plexus on rat tissue section is observed. Counter stained with DAPI.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at 1/1000 dilution.
Perform heat mediated antigen retrieval using Tris-EDTA (pH 9.0) (ab94681).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab188200).