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AB109284

Anti-IGFBP2 antibody [EPR3380(2)]

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(11 Publications)

Rabbit Recombinant Monoclonal IGFBP2 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 11 publications.

View Alternative Names

BP2, IBP2, IGFBP2, Insulin-like growth factor-binding protein 2, IBP-2, IGF-binding protein 2, IGFBP-2

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGFBP2 antibody [EPR3380(2)] (AB109284)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGFBP2 antibody [EPR3380(2)] (AB109284)

Immunohistochemical analysis of paraffin-embedded endometrial adenocarcinoma tissue with ab109284 at 1/100 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGFBP2 antibody [EPR3380(2)] (AB109284)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGFBP2 antibody [EPR3380(2)] (AB109284)

Immunohistochemical analysis of paraffin-embedded kidney tissue using 1/100 ab109284.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Western blot - Anti-IGFBP2 antibody [EPR3380(2)] (AB109284)
  • WB

Unknown

Western blot - Anti-IGFBP2 antibody [EPR3380(2)] (AB109284)

All lanes:

Western blot - Anti-IGFBP2 antibody [EPR3380(2)] (ab109284) at 1/10000 dilution

Lane 1:

T47-D cell lysate at 10 µg

Lane 2:

SH-SY5Y cell lysate at 10 µg

Lane 3:

BxPC-3 cell lysate at 10 µg

Lane 4:

A549 cell lysate at 10 µg

Lane 5:

NCCIT cell lysate at 10 µg

Predicted band size: 35 kDa

false

  • Carrier free

    Anti-IGFBP2 antibody [EPR3380(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3380(2)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p>" } } }
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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Multifunctional protein that plays a critical role in regulating the availability of IGFs such as IGF1 and IGF2 to their receptors and thereby regulates IGF-mediated cellular processes including proliferation, differentiation, and apoptosis in a cell-type specific manner (PubMed : 18563800, PubMed : 38796567). Functions coordinately with receptor protein tyrosine phosphatase beta/PTPRB and the IGF1 receptor to regulate IGF1-mediated signaling by stimulating the phosphorylation of PTEN leading to its inactivation and AKT1 activation (PubMed : 22869525). Plays a positive role in cell migration via interaction with integrin alpha5/ITGA5 through an RGD motif (PubMed : 16569642). Additionally, interaction with ITGA5/ITGB1 enhances the adhesion of endothelial progenitor cells to endothelial cells (PubMed : 26076738). Upon mitochondrial damage, facilitates apoptosis with ITGA5 of podocytes, and then activates the phosphorylation of focal adhesion kinase (FAK)-mediated mitochondrial injury (PubMed : 38796567).
See full target information IGFBP2
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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in pharmacology 16:1559546 PubMed40115255

2025

Targeting glycolysis in esophageal squamous cell carcinoma: single-cell and multi-omics insights for risk stratification and personalized therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Wang,Yunjie Shi,Xiao Hu,Chenfang Wang

Molecular cancer 22:206 PubMed38093346

2023

Dynamic altruistic cooperation within breast tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Muhammad Sufyan Bin Masroni,Kee Wah Lee,Victor Kwan Min Lee,Siok Bian Ng,Chao Teng Law,Kok Siong Poon,Bernett Teck-Kwong Lee,Zhehao Liu,Yuen Peng Tan,Wee Ling Chng,Steven Tucker,Lynette Su-Mien Ngo,George Wai Cheong Yip,Min En Nga,Susan Swee Shan Hue,Thomas Choudary Putti,Boon Huat Bay,Qingsong Lin,Lihan Zhou,Mikael Hartman,Tze Ping Loh,Manikandan Lakshmanan,Sook Yee Lee,Vinay Tergaonkar,Huiwen Chua,Adeline Voon Hui Lee,Eric Yew Meng Yeo,Mo-Huang Li,Chan Fong Chang,Zizheng Kee,Karen Mei-Ling Tan,Soo Yong Tan,Evelyn Siew-Chuan Koay,Marco Archetti,Sai Mun Leong

Translational oncology 34:101698 PubMed37307644

2023

A role for androgen receptor variant 7 in sensitivity to therapy: Involvement of IGFBP-2 and FOXA1.

Applications

Unspecified application

Species

Unspecified reactive species

K M Biernacka,R Barker,A Sewell,A Bahl,C M Perks

Oncology letters 22:697 PubMed34457052

2021

A micro-peptide encoded by HOXB-AS3 promotes the proliferation and viability of oral squamous cell carcinoma cell lines by directly binding with IGF2BP2 to stabilize c-Myc.

Applications

Unspecified application

Species

Unspecified reactive species

Fei Leng,Yan-Yu Miu,Yi Zhang,Hao Luo,Xiao-Li Lu,Hui Cheng,Zhi-Guo Zheng

Anticancer research 39:2829-2837 PubMed31177120

2019

IGFBP1 Is a Predictive Factor for Haematogenous Metastasis in Patients With Gastric Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yuya Sato,Mikito Inokuchi,Yoko Takagi,Kazuyuki Kojima

International journal of oncology 53:2397-2408 PubMed30272281

2018

Downregulation of CD147 induces malignant melanoma cell apoptosis via the regulation of IGFBP2 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Shuang Zhao,Lisha Wu,Yehong Kuang,Juan Su,Zhongling Luo,Yan Wang,Jinmao Li,Jianglin Zhang,Wangqing Chen,Fangfang Li,Yijing He,Juan Tao,Jianda Zhou,Xiaowei Xu,Cong Peng,Xiang Chen

Nature communications 9:3440 PubMed30143629

2018

SIRT6 haploinsufficiency induces BRAF melanoma cell resistance to MAPK inhibitors via IGF signalling.

Applications

Unspecified application

Species

Unspecified reactive species

Thomas Strub,Flavia G Ghiraldini,Saul Carcamo,Man Li,Aleksandra Wroblewska,Rajendra Singh,Matthew S Goldberg,Dan Hasson,Zichen Wang,Stuart J Gallagher,Peter Hersey,Avi Ma'ayan,Georgina V Long,Richard A Scolyer,Brian Brown,Bin Zheng,Emily Bernstein

Clinical cancer research : an official journal of 24:3217-3228 PubMed29476020

2018

Vitamin D-Binding Protein Enhances Epithelial Ovarian Cancer Progression by Regulating the Insulin-like Growth Factor-1/Akt Pathway and Vitamin D Receptor Transcription.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Fang Huang,Yi-Hui Wu,Wen-Fang Cheng,Shu-Ling Peng,Wan-Lin Shen,Cheng-Yang Chou

Molecular medicine reports 17:4383-4391 PubMed29344663

2018

Adeno-associated virus type 2-mediated gene transfer of a short hairpin-RNA targeting human IGFBP-2 suppresses the proliferation and invasion of MDA-MB-468 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Chao Gao,Ru-Song Zhang,Nan Zheng,Chen Wang

Clinical epigenetics 8:11 PubMed26823688

2016

Insulin-like growth factor axis in pregnancies affected by fetal growth disorders.

Applications

WB

Species

Human

Aamod R Nawathe,Mark Christian,Sung Hye Kim,Mark Johnson,Makrina D Savvidou,Vasso Terzidou
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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