Rabbit Recombinant Monoclonal IGFBP2 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 8 publications.
Images
![Western blot - Anti-IGFBP2 antibody [EPR3380(2)] (AB109284), expandable thumbnail](https://content.abcam.com/products/images/igfbp2-antibody-epr33802-ab109284--western-blot-img106063.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-IGFBP2 antibody [EPR3380(2)] (AB109284)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGFBP2 antibody [EPR3380(2)] (AB109284), expandable thumbnail](https://content.abcam.com/products/images/igfbp2-antibody-epr33802-ab109284--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img120328.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGFBP2 antibody [EPR3380(2)] (AB109284)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGFBP2 antibody [EPR3380(2)] (AB109284), expandable thumbnail](https://content.abcam.com/products/images/igfbp2-antibody-epr33802-ab109284--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img112675.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGFBP2 antibody [EPR3380(2)] (AB109284)")
Publications
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- Molecular cancer 22:2062023Dynamic altruistic cooperation within breast tumors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMuhammad Sufyan Bin Masroni,Kee Wah Lee,Victor Kwan Min Lee,Siok Bian Ng,Chao Teng Law,Kok Siong Poon,Bernett Teck-Kwong Lee,Zhehao Liu,Yuen Peng Tan,Wee Ling Chng,Steven Tucker,Lynette Su-Mien Ngo,George Wai Cheong Yip,Min En Nga,Susan Swee Shan Hue,Thomas Choudary Putti,Boon Huat Bay,Qingsong Lin,Lihan Zhou,Mikael Hartman,Tze Ping Loh,Manikandan Lakshmanan,Sook Yee Lee,Vinay Tergaonkar,Huiwen Chua,Adeline Voon Hui Lee,Eric Yew Meng Yeo,Mo-Huang Li,Chan Fong Chang,Zizheng Kee,Karen Mei-Ling Tan,Soo Yong Tan,Evelyn Siew-Chuan Koay,Marco Archetti,Sai Mun LeongPubMed 38093346
- Anticancer research 39:2829-28372019IGFBP1 Is a Predictive Factor for Haematogenous Metastasis in Patients With Gastric Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuya Sato,Mikito Inokuchi,Yoko Takagi,Kazuyuki KojimaPubMed 31177120
- International journal of oncology 53:2397-24082018Downregulation of CD147 induces malignant melanoma cell apoptosis via the regulation of IGFBP2 expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShuang Zhao,Lisha Wu,Yehong Kuang,Juan Su,Zhongling Luo,Yan Wang,Jinmao Li,Jianglin Zhang,Wangqing Chen,Fangfang Li,Yijing He,Juan Tao,Jianda Zhou,Xiaowei Xu,Cong Peng,Xiang ChenPubMed 30272281
- Nature communications 9:34402018SIRT6 haploinsufficiency induces BRAF melanoma cell resistance to MAPK inhibitors via IGF signalling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesThomas Strub et. al.PubMed 30143629
- Clinical cancer research : an official journal of 24:3217-32282018Vitamin D-Binding Protein Enhances Epithelial Ovarian Cancer Progression by Regulating the Insulin-like Growth Factor-1/Akt Pathway and Vitamin D Receptor Transcription.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu-Fang Huang et. al.PubMed 29476020
- Molecular medicine reports 17:4383-43912018Adeno-associated virus type 2-mediated gene transfer of a short hairpin-RNA targeting human IGFBP-2 suppresses the proliferation and invasion of MDA-MB-468 cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChao Gao et. al.PubMed 29344663
- Clinical epigenetics 8:112016Insulin-like growth factor axis in pregnancies affected by fetal growth disorders.
- PloS one 10:e01193482015Human adipocytes stimulate invasion of breast cancer MCF-7 cells by secreting IGFBP-2.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | Flow Cyt | WB | IHC-P | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Tested | Tested |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10000 - 1/50000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/250 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Function
Multifunctional protein that plays a critical role in regulating the availability of IGFs such as IGF1 and IGF2 to their receptors and thereby regulates IGF-mediated cellular processes including proliferation, differentiation, and apoptosis in a cell-type specific manner (PubMed:18563800, PubMed:38796567). Functions coordinately with receptor protein tyrosine phosphatase beta/PTPRB and the IGF1 receptor to regulate IGF1-mediated signaling by stimulating the phosphorylation of PTEN leading to its inactivation and AKT1 activation (PubMed:22869525). Plays a positive role in cell migration via interaction with integrin alpha5/ITGA5 through an RGD motif (PubMed:16569642). Additionally, interaction with ITGA5/ITGB1 enhances the adhesion of endothelial progenitor cells to endothelial cells (PubMed:26076738). Upon mitochondrial damage, facilitates apoptosis with ITGA5 of podocytes, and then activates the phosphorylation of focal adhesion kinase (FAK)-mediated mitochondrial injury (PubMed:38796567).
Alternative names
BP2, IBP2, IGFBP2, Insulin-like growth factor-binding protein 2, IBP-2, IGF-binding protein 2, IGFBP-2
Recommended products
Rabbit Recombinant Monoclonal IGFBP2 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 8 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR3380(2)
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Stable for 12 months at -20°C
Notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-IGFBP2 antibody [EPR3380(2)] (ab109284), expandable thumbnail](https://content.abcam.com/products/images/igfbp2-antibody-epr33802-ab109284--western-blot-img106063.jpg "Western blot - Anti-IGFBP2 antibody [EPR3380(2)] (ab109284)")
Western blot - Anti-IGFBP2 antibody [EPR3380(2)] (ab109284)
All lanes: Western blot - Anti-IGFBP2 antibody [EPR3380(2)] (ab109284) at 1/10000 dilution
Lane 1: T47-D cell lysate at 10 µg
Lane 2: SH-SY5Y cell lysate at 10 µg
Lane 3: BxPC-3 cell lysate at 10 µg
Lane 4: A549 cell lysate at 10 µg
Lane 5: NCCIT cell lysate at 10 µg
Predicted band size: 35 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGFBP2 antibody [EPR3380(2)] (ab109284), expandable thumbnail](https://content.abcam.com/products/images/igfbp2-antibody-epr33802-ab109284--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img120328.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGFBP2 antibody [EPR3380(2)] (ab109284)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGFBP2 antibody [EPR3380(2)] (ab109284)
Immunohistochemical analysis of paraffin-embedded kidney tissue using 1/100 ab109284.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGFBP2 antibody [EPR3380(2)] (ab109284), expandable thumbnail](https://content.abcam.com/products/images/igfbp2-antibody-epr33802-ab109284--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img112675.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGFBP2 antibody [EPR3380(2)] (ab109284)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGFBP2 antibody [EPR3380(2)] (ab109284)
Immunohistochemical analysis of paraffin-embedded endometrial adenocarcinoma tissue with ab109284 at 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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