AB217205

Anti-IGFBP3 antibody

Name: Anti-IGFBP3 antibody (ab217205) | Abcam
Brand: Abcam Limited
SKU: AB217205
Price: 545 USD
Availability: InStock

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(4 Publications)

Rabbit Polyclonal IGFBP3 antibody. Suitable for WB, IHC-P and reacts with Rat samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human IGFBP3 aa 200 to C-terminus conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

IBP3, IGFBP3, Insulin-like growth factor-binding protein 3, IBP-3, IGF-binding protein 3, IGFBP-3

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGFBP3 antibody (AB217205)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGFBP3 antibody (AB217205)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat brain tissue labeling IGFBP3 with ab217205 at 1/200 dilution overnight at 4°C, followed by Goat Anti-Rabbit IgG, Cy3 conjugated antibody at 1/200 dilution for 40 minutes at 37°C.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGFBP3 antibody (AB217205)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat brain tissue labeling IGFBP3 with ab217205 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

Supplier Data

Western blot - Anti-IGFBP3 antibody (AB217205)

12% Gel

All lanes:

Western blot - Anti-IGFBP3 antibody (ab217205) at 1/200 dilution

Lane 1:

Rat kidney lysate

Lane 2:

Rat brain lysate

Secondary

All lanes:

Goat Anti-Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated at 1/3000 dilution

Predicted band size: 32 kDa

Observed band size: 30 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human IGFBP3 aa 200 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P17936

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p>If a fluorescent secondary antibody is used the recommended dilution is 1/50 - 1/200.</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Multifunctional protein that plays a critical role in regulating the availability of IGFs such as IGF1 and IGF2 to their receptors and thereby regulates IGF-mediated cellular processes including proliferation, differentiation, and apoptosis in a cell-type specific manner (PubMed : 10874028, PubMed : 19556345). Also exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R (PubMed : 20353938). Inhibits the positive effect of humanin on insulin sensitivity (PubMed : 19623253). Promotes testicular germ cell apoptosis (PubMed : 19952275). Acts via LRP-1/alpha2M receptor, also known as TGF-beta type V receptor, to mediate cell growth inhibition independent of IGF1 (PubMed : 9252371). Mechanistically, induces serine-specific dephosphorylation of IRS1 or IRS2 upon ligation to its receptor, leading to the inhibitory cascade (PubMed : 15371331). In the nucleus, interacts with transcription factors such as retinoid X receptor-alpha/RXRA to regulate transcriptional signaling and apoptosis (PubMed : 10874028).

See full target information IGFBP3

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Immunity, inflammation and disease 12:e70072 PubMed39555740

2024

Senescence in Intervertebral Disc Degeneration: A Comprehensive Analysis Based on Bioinformatic Strategies.

Applications

Unspecified application

Species

Unspecified reactive species

Zijun Zhao,Yining Wang,Zairan Wang,Fan Zhang,Ze Ding,Tao Fan

Biology direct 18:34 PubMed37365579

2023

Dynamic expression of IGFBP3 modulate dual actions of mineralization micro-environment during tooth development via Wnt/beta-catenin signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

MengDan Zhang,Junming Zheng,Siyuan Wu,Hailing Chen,Lusai Xiang

Regenerative therapy 11:217-224 PubMed31516919

2019

The putative role of insulin-like growth factor (IGF)-binding protein 5 independent of IGF in the maintenance of pulpal homeostasis in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Kotaro Saito,Hayato Ohshima

Journal of oral biosciences 61:157-162 PubMed31400542

2019

Regulation of IGF-I by IGFBP3 and IGFBP5 during odontoblast differentiation in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Chisato Aizawa,Kotaro Saito,Hayato Ohshima

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-IGFBP3 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGFBP3 antibody (AB217205)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGFBP3 antibody (AB217205)

Western blot - Anti-IGFBP3 antibody (AB217205)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (4)

Product promise