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AB272857

Anti-IGFBP3 antibody

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Rabbit Polyclonal IGFBP3 antibody. Suitable for WB, IHC-Fr and reacts with Mouse, Rat, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human IGFBP3.

View Alternative Names

IBP3, IGFBP3, Insulin-like growth factor-binding protein 3, IBP-3, IGF-binding protein 3, IGFBP-3

2 Images
Immunohistochemistry (Frozen sections) - Anti-IGFBP3 antibody (AB272857)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-IGFBP3 antibody (AB272857)

Frozen sections of E13.5 rat brain tissue stained for IGFBP3 (green) using ab272857 at 1/250 dilution in immunohistochemical analysis.

Counterstain : beta Tubulin 3/ TUJ1, a mature neuron marker (red). Fluoroshield™ with DAPI (blue).

Western blot - Anti-IGFBP3 antibody (AB272857)
  • WB

Supplier Data

Western blot - Anti-IGFBP3 antibody (AB272857)

12% SDS-PAGE

All lanes:

Western blot - Anti-IGFBP3 antibody (ab272857) at 1/1000 dilution

All lanes:

Mouse plasma at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 32 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-Fr

applications

Immunogen

Recombinant Fragment Protein within Human IGFBP3. The exact immunogen used to generate this antibody is proprietary information.

P17936

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/100 - 1/1000", "IHCFr-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Multifunctional protein that plays a critical role in regulating the availability of IGFs such as IGF1 and IGF2 to their receptors and thereby regulates IGF-mediated cellular processes including proliferation, differentiation, and apoptosis in a cell-type specific manner (PubMed : 10874028, PubMed : 19556345). Also exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R (PubMed : 20353938). Inhibits the positive effect of humanin on insulin sensitivity (PubMed : 19623253). Promotes testicular germ cell apoptosis (PubMed : 19952275). Acts via LRP-1/alpha2M receptor, also known as TGF-beta type V receptor, to mediate cell growth inhibition independent of IGF1 (PubMed : 9252371). Mechanistically, induces serine-specific dephosphorylation of IRS1 or IRS2 upon ligation to its receptor, leading to the inhibitory cascade (PubMed : 15371331). In the nucleus, interacts with transcription factors such as retinoid X receptor-alpha/RXRA to regulate transcriptional signaling and apoptosis (PubMed : 10874028).
See full target information IGFBP3
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Product promise

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