AB224530

Anti-IGFBP3 antibody [EPR18680-153] - BSA and Azide free

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal IGFBP3 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications.

View Alternative Names

IBP3, IGFBP3, Insulin-like growth factor-binding protein 3, IBP-3, IGF-binding protein 3, IGFBP-3

2 Images

Supplier Data

Immunoprecipitation - Anti-IGFBP3 antibody [EPR18680-153] - BSA and Azide free (AB224530)

IGFBP3 was immunoprecipitated from 0.35 mg of human serum with ab193910 at 1/70 dilution. Western blot was performed from the immunoprecipitate using ab193910 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Lane 1 : Human serum 10 μg (Input).

Lane 2 : ab193910 IP in human serum.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab193910 in human serum.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 seconds.

The bands at 41 kDa and 44 kDa have previously been documented as IGFBP3 doublets (PMID : 16816363).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab193910).

All lanes:

Immunoprecipitation - Anti-IGFBP3 antibody [EPR18680-153] (<a href='/en-us/products/primary-antibodies/igfbp3-antibody-epr18680-153-ab193910'>ab193910</a>)

Predicted band size: 32 kDa

Observed band size: 32 kDa

true

Supplier Data

Western blot - Anti-IGFBP3 antibody [EPR18680-153] - BSA and Azide free (AB224530)

Blocking and dilution buffer : 5% NFDM/TBST.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab193910).

All lanes:

Western blot - Anti-IGFBP3 antibody [EPR18680-153] (<a href='/en-us/products/primary-antibodies/igfbp3-antibody-epr18680-153-ab193910'>ab193910</a>) at 1/1000 dilution

All lanes:

Human serum at 10 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/2000 dilution

Predicted band size: 32 kDa

Observed band size: 32 kDa

true

Exposure time: 10s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR18680-153

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }

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Product details

ab224530 is the carrier-free version of ab193910.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Multifunctional protein that plays a critical role in regulating the availability of IGFs such as IGF1 and IGF2 to their receptors and thereby regulates IGF-mediated cellular processes including proliferation, differentiation, and apoptosis in a cell-type specific manner (PubMed : 10874028, PubMed : 19556345). Also exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R (PubMed : 20353938). Inhibits the positive effect of humanin on insulin sensitivity (PubMed : 19623253). Promotes testicular germ cell apoptosis (PubMed : 19952275). Acts via LRP-1/alpha2M receptor, also known as TGF-beta type V receptor, to mediate cell growth inhibition independent of IGF1 (PubMed : 9252371). Mechanistically, induces serine-specific dephosphorylation of IRS1 or IRS2 upon ligation to its receptor, leading to the inhibitory cascade (PubMed : 15371331). In the nucleus, interacts with transcription factors such as retinoid X receptor-alpha/RXRA to regulate transcriptional signaling and apoptosis (PubMed : 10874028).

See full target information IGFBP3

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 13:5374 PubMed37005468

2023

Spatial subcellular organelle networks in single cells.

Applications

Unspecified application

Species

Unspecified reactive species

Mythreye Venkatesan,Nicholas Zhang,Benoit Marteau,Yukina Yajima,Nerea Ortiz De Zarate Garcia,Zhou Fang,Thomas Hu,Shuangyi Cai,Adam Ford,Harrison Olszewski,Andrew Borst,Ahmet F Coskun

Nature aging 1:698-714 PubMed34746803

2021

Senescent cells suppress innate smooth muscle cell repair functions in atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Bennett G Childs,Cheng Zhang,Fahad Shuja,Ines Sturmlechner,Shawn Trewartha,Raul Fierro Velasco,Darren Baker,Hu Li,Jan M van Deursen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-IGFBP3 antibody [EPR18680-153] - BSA and Azide free

Immunoprecipitation - Anti-IGFBP3 antibody [EPR18680-153] - BSA and Azide free (AB224530)

Western blot - Anti-IGFBP3 antibody [EPR18680-153] - BSA and Azide free (AB224530)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (2)

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