Anti-IGFBP3 antibody [EPR22152-22] - BSA and Azide free

• WB

Supplier Data

Western blot - Anti-IGFBP3 antibody [EPR22152-22] - BSA and Azide free (AB239789)

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure times.

Lane 1 : 8 seconds, Lane 2 : 81 seconds, Lane 3 : 3 minutes

IGFBP3 exists as both glycosylated (46 kDa) and non-glycosylated (29 kDa) form. The molecular weight observed is consistent with what has been described in the literature (PMID 10352394; PMID : 9698597; PMID 22910030).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab220429).

All lanes:

Western blot - Anti-IGFBP3 antibody [EPR22152-22] (<a href='/en-us/products/primary-antibodies/igfbp3-antibody-epr22152-22-ab220429'>ab220429</a>) at 1/1000 dilution

Lane 1:

Mouse lung primary cell culture supernatant at 20 µL

Lane 2:

Mouse serum at 20 µg

Lane 3:

Rat serum at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 32 kDa

Observed band size: 29 kDa,46 kDa

false

• IP

Unknown

Immunoprecipitation - Anti-IGFBP3 antibody [EPR22152-22] - BSA and Azide free (AB239789)

IGFBP3 was immunoprecipitated from 0.35 mg of rat serum with ab220429 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab220429 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

Lane 1 : Rat serum 10 μg (Input).
Lane 2 : ab220429 IP in rat serum (+).
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab20429 in rat serum.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 30 seconds.

Both glycosylated (46 kDa) and non-glycosylated (29 kDa) IGFBP-3 are detected in rat serum. The molecular weight observed is consistent with what has been described in the literature (PMID 10352394; PMID : 9698597).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab220429).

All lanes:

Immunoprecipitation - Anti-IGFBP3 antibody [EPR22152-22] (<a href='/en-us/products/primary-antibodies/igfbp3-antibody-epr22152-22-ab220429'>ab220429</a>)

Predicted band size: 32 kDa

Observed band size: 29 kDa,46 kDa

false

• IP

Unknown

Immunoprecipitation - Anti-IGFBP3 antibody [EPR22152-22] - BSA and Azide free (AB239789)

IGFBP3 was immunoprecipitated from 0.35 mg of mouse serum with ab220429 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab220429 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

Lane 1 : Mouse serum 10 μg (Input).
Lane 2 : ab220429 IP in mouse serum (+).
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab20429 in mouse serum.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 30 seconds.

Non-glycosylated IGFBP-3 (29 kDa) is detected in mouse serum. The molecular weight observed is consistent with what has been described in the literature (PMID 10352394).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab220429).

All lanes:

Immunoprecipitation - Anti-IGFBP3 antibody [EPR22152-22] (<a href='/en-us/products/primary-antibodies/igfbp3-antibody-epr22152-22-ab220429'>ab220429</a>)

Predicted band size: 32 kDa

Observed band size: 29 kDa

false