AB205581

Anti-IGFBP4 antibody [EPR19762]

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal IGFBP4 antibody. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications.

View Alternative Names

IBP4, IGFBP4, Insulin-like growth factor-binding protein 4, IBP-4, IGF-binding protein 4, IGFBP-4

5 Images

Flow Cytometry (Intracellular) - Anti-IGFBP4 antibody [EPR19762] (AB205581)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-IGFBP4 antibody [EPR19762] (AB205581)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed A549 (human lung carcinoma cell line) cell line labeling IGFBP4 with ab205581 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/2000 dilution was used as the secondary antibody.

Supplier Data

Immunoprecipitation - Anti-IGFBP4 antibody [EPR19762] (AB205581)

IGFBP4 was immunoprecipitated from 0.35 mg of A549 (human lung carcinoma cell line) whole cell lysate with ab205581 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab205581 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : A549 whole cell lysate 10 μg (Input).

Lane 2 : ab205581 IP in A549 whole cell lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab205581 in A549 whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 1 second.

Note : Human IGFBP4 has 2 isoforms detected at 28 kDa and 18 kDa.

All lanes:

Immunoprecipitation - Anti-IGFBP4 antibody [EPR19762] (ab205581)

Predicted band size: 28 kDa

false

Supplier Data

Western blot - Anti-IGFBP4 antibody [EPR19762] (AB205581)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-IGFBP4 antibody [EPR19762] (ab205581) at 1/1000 dilution

All lanes:

Human placenta tissue lysate at 10 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 28 kDa

Observed band size: 28 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-IGFBP4 antibody [EPR19762] (AB205581)

Blocking/Dilution buffer : 5% NFDM/TBST.

Human IGFBP4 has 2 isoforms detected at 28 kDa and 18 kDa.

All lanes:

Western blot - Anti-IGFBP4 antibody [EPR19762] (ab205581) at 1/1000 dilution

All lanes:

A549 (human lung carcinoma cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 28 kDa

Observed band size: 18 kDa,28 kDa

false

Exposure time: 3s

Supplier Data

Western blot - Anti-IGFBP4 antibody [EPR19762] (AB205581)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-IGFBP4 antibody [EPR19762] (ab205581) at 1/1000 dilution

All lanes:

HT-29 (human colorectal adenocarcinoma cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 28 kDa,504 kDa

Observed band size: 28 kDa,85 kDa

false

Exposure time: 3min

Carrier free

Anti-IGFBP4 antibody [EPR19762] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19762

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.

See full target information IGFBP4

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell discovery 8:137 PubMed36550107

2022

Structural insights into the covalent regulation of PAPP-A activity by proMBP and STC2.

Applications

Unspecified application

Species

Unspecified reactive species

Qihang Zhong,Honglei Chu,Guopeng Wang,Cheng Zhang,Rong Li,Fusheng Guo,Xinlu Meng,Xiaoguang Lei,Youli Zhou,Ruobing Ren,Lin Tao,Ningning Li,Ning Gao,Yuan Wei,Jie Qiao,Jing Hang

Scientific reports 10:18829 PubMed33139779

2020

Twist2 is NFkB-responsive when p120-catenin is inactivated and EGFR is overexpressed in esophageal keratinocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Heather L Lehman,Michal Kidacki,Douglas B Stairs

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-IGFBP4 antibody [EPR19762]

Flow Cytometry (Intracellular) - Anti-IGFBP4 antibody [EPR19762] (AB205581)

Immunoprecipitation - Anti-IGFBP4 antibody [EPR19762] (AB205581)

Western blot - Anti-IGFBP4 antibody [EPR19762] (AB205581)

Western blot - Anti-IGFBP4 antibody [EPR19762] (AB205581)

Western blot - Anti-IGFBP4 antibody [EPR19762] (AB205581)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (2)

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Product promise