Anti-IGFBP4 antibody [EPR19762] - BSA and Azide free

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-IGFBP4 antibody [EPR19762] - BSA and Azide free (AB251435)

This data was developed using ab205581, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed A549 (human lung carcinoma cell line) cell line labeling IGFBP4 with ab205581 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/2000 dilution was used as the secondary antibody.

• IP

Supplier Data

Immunoprecipitation - Anti-IGFBP4 antibody [EPR19762] - BSA and Azide free (AB251435)

This data was developed using ab205581, the same antibody clone in a different buffer formulation.

IGFBP4 was immunoprecipitated from 0.35 mg of A549 (human lung carcinoma cell line) whole cell lysate with ab205581 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab205581 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : A549 whole cell lysate 10 μg (Input).
Lane 2 : ab205581 IP in A549 whole cell lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab205581 in A549 whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 1 second.

Note : Human IGFBP4 has 2 isoforms detected at 28 kDa and 18 kDa.

All lanes:

Immunoprecipitation - Anti-IGFBP4 antibody [EPR19762] (<a href='/en-us/products/primary-antibodies/igfbp4-antibody-epr19762-ab205581'>ab205581</a>)

Predicted band size: 28 kDa

false

• WB

Supplier Data

Western blot - Anti-IGFBP4 antibody [EPR19762] - BSA and Azide free (AB251435)

This data was developed using ab205581, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-IGFBP4 antibody [EPR19762] (<a href='/en-us/products/primary-antibodies/igfbp4-antibody-epr19762-ab205581'>ab205581</a>) at 1/1000 dilution

All lanes:

Human placenta tissue lysate at 10 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 28 kDa

Observed band size: 28 kDa

false

Exposure time: 3min

• WB

Supplier Data

Western blot - Anti-IGFBP4 antibody [EPR19762] - BSA and Azide free (AB251435)

This data was developed using ab205581, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Human IGFBP4 has 2 isoforms detected at 28 kDa and 18 kDa.

All lanes:

Western blot - Anti-IGFBP4 antibody [EPR19762] (<a href='/en-us/products/primary-antibodies/igfbp4-antibody-epr19762-ab205581'>ab205581</a>) at 1/1000 dilution

All lanes:

A549 (human lung carcinoma cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 28 kDa

Observed band size: 18 kDa,28 kDa

false

Exposure time: 3s

• WB

Supplier Data

Western blot - Anti-IGFBP4 antibody [EPR19762] - BSA and Azide free (AB251435)

This data was developed using ab205581, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-IGFBP4 antibody [EPR19762] (<a href='/en-us/products/primary-antibodies/igfbp4-antibody-epr19762-ab205581'>ab205581</a>) at 1/1000 dilution

All lanes:

HT-29 (human colorectal adenocarcinoma cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 28 kDa,504 kDa

Observed band size: 28 kDa,85 kDa

false

Exposure time: 3min