Rabbit Recombinant Monoclonal IGFBP5 antibody. Suitable for IP, WB and reacts with Mouse, Rat samples. Cited in 4 publications.
Images
![Western blot - Anti-IGFBP5 antibody [EPR18013-137] (AB254324), expandable thumbnail](https://content.abcam.com/products/images/igfbp5-antibody-epr18013-137-ab254324--western-blot-img7805.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-IGFBP5 antibody [EPR18013-137] (AB254324)")
![Western blot - Anti-IGFBP5 antibody [EPR18013-137] (AB254324), expandable thumbnail](https://content.abcam.com/products/images/igfbp5-antibody-epr18013-137-ab254324--western-blot-img84724.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-IGFBP5 antibody [EPR18013-137] (AB254324)")
![Immunoprecipitation - Anti-IGFBP5 antibody [EPR18013-137] (AB254324), expandable thumbnail](https://content.abcam.com/products/images/igfbp5-antibody-epr18013-137-ab254324--immunoprecipitation-img11039.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-IGFBP5 antibody [EPR18013-137] (AB254324)")
![Western blot - Anti-IGFBP5 antibody [EPR18013-137] (AB254324), expandable thumbnail](https://content.abcam.com/products/images/igfbp5-antibody-epr18013-137-ab254324--western-blot-img9830.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-IGFBP5 antibody [EPR18013-137] (AB254324)")
Publications
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- Cell death & disease 14:8262023The chromatin remodeling protein BRG1 regulates HSC-myofibroblast differentiation and liver fibrosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuwen Zhu,Aoqi Kang,Yameng Kuai,Yan Guo,Xiulian Miao,Li Zhu,Ming Kong,Nan LiPubMed 38092723
- Acta biochimica et biophysica Sinica 55:548-5602023Transcriptome analysis reveals a potential regulatory mechanism of the lnc-5423.6/IGFBP5 axis in the early stages of mouse thymic involution.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBingxin Li,Yaqiong Ye,Longsheng Hong,Wanyan Li,Qingru Wu,Wenjun Liu,Yongjiang Ma,Danning Xu,Yugu LiPubMed 37078747
- Human genomics 15:502021LncRNA-TUG1 promotes the progression of infantile hemangioma by regulating miR-137/IGFBP5 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLili Zhou,Xiao Jia,Xiangzheng YangPubMed 34362467
- International journal of molecular sciences 21:2020Dual Leucine Zipper Kinase Is Constitutively Active in the Adult Mouse Brain and Has Both Stress-Induced and Homeostatic Functions.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Abcam Recommends
Reactivity data
Select an application| IP | Flow Cyt | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|---|
| Mouse | Tested | Not recommended | Tested | Not recommended | Not recommended |
| Rat | Expected | Not recommended | Tested | Not recommended | Not recommended |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/30 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Multifunctional protein that plays a critical role in regulating the availability of IGFs to their receptors and thereby regulates IGF-mediated cellular processes including proliferation, differentiation, and apoptosis in a cell-type specific manner. Increases the cell proliferation of osteoblasts, intestinal smooth muscle cells and neuroblastoma cells (By similarity). Enhances adhesion and survival of epithelial cells but decreases adhesion of mesenchymal cells (PubMed:24120850). Once secreted, acts as a major mediator of mTORC1-dependent feedback inhibition of IGF1 signaling (By similarity). Plays also a role in the induction of extracellular matrix (ECM) production and deposition independently of its nuclear translocation and binding to IGFs. Acts itself as a growth factor that can act independently of IGFs to regulate bone formation. Acts as a ligand for the ROR1 receptor which triggers formation of ROR1/HER2 heterodimer to enhance CREB oncogenic signaling (By similarity).
Alternative names
Igfbp-5, Igfbp5, Insulin-like growth factor-binding protein 5, IBP-5, IGF-binding protein 5, IGFBP-5
Recommended products
Rabbit Recombinant Monoclonal IGFBP5 antibody. Suitable for IP, WB and reacts with Mouse, Rat samples. Cited in 4 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR18013-137
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-IGFBP5 antibody [EPR18013-137] (ab254324), expandable thumbnail](https://content.abcam.com/products/images/igfbp5-antibody-epr18013-137-ab254324--western-blot-img7805.jpg "Western blot - Anti-IGFBP5 antibody [EPR18013-137] (ab254324)")
Western blot - Anti-IGFBP5 antibody [EPR18013-137] (ab254324)
IGFBP5 can be processed into approximately 16 kDa fragment. The molecular weight observed is consistent with what has been described in the literature (PMID: 11701437, PMID:11920405).
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 92 seconds
All lanes: Western blot - Anti-IGFBP5 antibody [EPR18013-137] (ab254324) at 1/1000 dilution
All lanes: Rat uterus tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution
Predicted band size: 31 kDa
Observed band size: 16 kDa, 34 kDa
![Western blot - Anti-IGFBP5 antibody [EPR18013-137] (ab254324), expandable thumbnail](https://content.abcam.com/products/images/igfbp5-antibody-epr18013-137-ab254324--western-blot-img84724.jpg "Western blot - Anti-IGFBP5 antibody [EPR18013-137] (ab254324)")
Western blot - Anti-IGFBP5 antibody [EPR18013-137] (ab254324)
IGFBP5 can be processed into approximately 16 kDa fragment. The molecular weight observed is consistent with what has been described in the literature (PMID: 11701437, PMID:11920405).
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 3 minutes
All lanes: Western blot - Anti-IGFBP5 antibody [EPR18013-137] (ab254324) at 1/1000 dilution
All lanes: Mouse lung tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 31 kDa
![Immunoprecipitation - Anti-IGFBP5 antibody [EPR18013-137] (ab254324), expandable thumbnail](https://content.abcam.com/products/images/igfbp5-antibody-epr18013-137-ab254324--immunoprecipitation-img11039.jpg "Immunoprecipitation - Anti-IGFBP5 antibody [EPR18013-137] (ab254324)")
Immunoprecipitation - Anti-IGFBP5 antibody [EPR18013-137] (ab254324)
IGFBP5 was immunoprecipitated from 0.35 mg C2C12 (Mouse myoblasts myoblast) whole cell lysate using ab254324 at 1/30 dilution. Western blot was perfromed from the immunoprecipitate using ab254324 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: C2C12 whole cell lysate 10 μg (input)
Lane 2: ab254324 IP in C2C12 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab254324 in C2C12 whole cell lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 minute.
All lanes: Immunoprecipitation - Anti-IGFBP5 antibody [EPR18013-137] (ab254324)
Predicted band size: 31 kDa
Observed band size: 16 kDa, 34 kDa
![Western blot - Anti-IGFBP5 antibody [EPR18013-137] (ab254324), expandable thumbnail](https://content.abcam.com/products/images/igfbp5-antibody-epr18013-137-ab254324--western-blot-img9830.jpg "Western blot - Anti-IGFBP5 antibody [EPR18013-137] (ab254324)")
Western blot - Anti-IGFBP5 antibody [EPR18013-137] (ab254324)
The level of IGFBP5 expression is elevated in C2C12 after differentiation. (PMID: 7693664)
IGFBP5 can be processed into approximately 16 kDa fragment. The molecular weight observed is consistent with what has been described in the literature (PMID: 11701437, PMID:11920405)
Exposure time: 7.75 seconds
Blocking/diluting buffer and concentration: 5% NFDM/TBST
All lanes: Western blot - Anti-IGFBP5 antibody [EPR18013-137] (ab254324) at 1/1000 dilution
Lane 1: Untreated C2C12 (mouse myoblasts myoblast), whole cell lysate
Lane 2: C2C12 treated with 2% horse serum for 6 days, whole cell lysate
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution
Predicted band size: 31 kDa
Observed band size: 16 kDa, 34 kDa
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