AB254324

Anti-IGFBP5 antibody [EPR18013-137]

RabMAb
Recombinant
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(7 Publications)

Rabbit Recombinant Monoclonal IGFBP5 antibody. Suitable for IP, WB and reacts with Mouse, Rat samples. Cited in 7 publications.

View Alternative Names

Igfbp-5, Igfbp5, Insulin-like growth factor-binding protein 5, IBP-5, IGF-binding protein 5, IGFBP-5

4 Images

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Immunoprecipitation - Anti-IGFBP5 antibody [EPR18013-137] (AB254324)

IGFBP5 was immunoprecipitated from 0.35 mg C2C12 (Mouse myoblasts myoblast) whole cell lysate using ab254324 at 1/30 dilution. Western blot was perfromed from the immunoprecipitate using ab254324 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : C2C12 whole cell lysate 10 μg (input)
Lane 2 : ab254324 IP in C2C12 whole cell lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab254324 in C2C12 whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 1 minute.

All lanes:

Immunoprecipitation - Anti-IGFBP5 antibody [EPR18013-137] (ab254324)

Predicted band size: 31 kDa

Observed band size: 16 kDa,34 kDa

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Western blot - Anti-IGFBP5 antibody [EPR18013-137] (AB254324)

IGFBP5 can be processed into approximately 16 kDa fragment. The molecular weight observed is consistent with what has been described in the literature (PMID : 11701437, PMID : 11920405).

Blocking/Diluting buffer and concentration : 5% NFDM/TBST

Exposure time : 3 minutes

All lanes:

Western blot - Anti-IGFBP5 antibody [EPR18013-137] (ab254324) at 1/1000 dilution

All lanes:

Mouse lung tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 31 kDa

false

Unknown

Western blot - Anti-IGFBP5 antibody [EPR18013-137] (AB254324)

The level of IGFBP5 expression is elevated in C2C12 after differentiation. (PMID : 7693664)

IGFBP5 can be processed into approximately 16 kDa fragment. The molecular weight observed is consistent with what has been described in the literature (PMID : 11701437, PMID : 11920405)

Exposure time : 7.75 seconds

Blocking/diluting buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-IGFBP5 antibody [EPR18013-137] (ab254324) at 1/1000 dilution

Lane 1:

Untreated C2C12 (mouse myoblasts myoblast), whole cell lysate

Lane 2:

C2C12 treated with 2% horse serum for 6 days, whole cell lysate

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 31 kDa

Observed band size: 16 kDa,34 kDa

false

Unknown

Western blot - Anti-IGFBP5 antibody [EPR18013-137] (AB254324)

IGFBP5 can be processed into approximately 16 kDa fragment. The molecular weight observed is consistent with what has been described in the literature (PMID : 11701437, PMID : 11920405).

Blocking/Diluting buffer and concentration : 5% NFDM/TBST

Exposure time : 92 seconds

All lanes:

Western blot - Anti-IGFBP5 antibody [EPR18013-137] (ab254324) at 1/1000 dilution

All lanes:

Rat uterus tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 31 kDa

Observed band size: 16 kDa,34 kDa

false

Carrier free

Anti-IGFBP5 antibody [EPR18013-137] - BSA and Azide free (Detector)

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR18013-137

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Multifunctional protein that plays a critical role in regulating the availability of IGFs to their receptors and thereby regulates IGF-mediated cellular processes including proliferation, differentiation, and apoptosis in a cell-type specific manner. Increases the cell proliferation of osteoblasts, intestinal smooth muscle cells and neuroblastoma cells (By similarity). Enhances adhesion and survival of epithelial cells but decreases adhesion of mesenchymal cells (PubMed : 24120850). Once secreted, acts as a major mediator of mTORC1-dependent feedback inhibition of IGF1 signaling (By similarity). Also plays a role in the induction of extracellular matrix (ECM) production and deposition independently of its nuclear translocation and binding to IGFs. Acts itself as a growth factor that can act independently of IGFs to regulate bone formation. Acts as a ligand for the ROR1 receptor which triggers formation of ROR1/HER2 heterodimer to enhance CREB oncogenic signaling (By similarity).

See full target information Igfbp5

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:23083 PubMed40593070

2025

Exercise ameliorates nonalcoholic fatty liver disease by reducing the IGFBP5 to IGF1 ratio to activate AMPK pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Yu,Guanxiong Zhu,Zeyu Zhang,Huiguo Wang,Liting Zeng,Jingbo Xia,Xiaoguang Liu,Chunlu Fang,Shujing Liu,Yunjie Yang,Lina Yu

Frontiers in pharmacology 16:1544869 PubMed40365313

2025

IGFBP5 mediates the therapeutic effect of isoliquiritigenin in myocardial ischemia-reperfusion injury via AKT/GLUT4 regulated insulin resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Jue Bai,Si-Yuan Yang,Shao-Mei Yu,Ying Cao,Chang-Han Ma,Xuan-Yi Hu,Xiong Chen,Ying-Nan Song,Hong-Jin Chen

Nature communications 15:5331 PubMed38909026

2024

TENT5-mediated polyadenylation of mRNAs encoding secreted proteins is essential for gametogenesis in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Michał Brouze,Agnieszka Czarnocka-Cieciura,Olga Gewartowska,Monika Kusio-Kobiałka,Kamil Jachacy,Marcin Szpila,Bartosz Tarkowski,Jakub Gruchota,Paweł Krawczyk,Seweryn Mroczek,Ewa Borsuk,Andrzej Dziembowski

Cell death & disease 14:826 PubMed38092723

2023

The chromatin remodeling protein BRG1 regulates HSC-myofibroblast differentiation and liver fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yuwen Zhu,Aoqi Kang,Yameng Kuai,Yan Guo,Xiulian Miao,Li Zhu,Ming Kong,Nan Li

Acta biochimica et biophysica Sinica 55:548-560 PubMed37078747

2023

Transcriptome analysis reveals a potential regulatory mechanism of the lnc-5423.6/IGFBP5 axis in the early stages of mouse thymic involution.

Applications

Unspecified application

Species

Unspecified reactive species

Bingxin Li,Yaqiong Ye,Longsheng Hong,Wanyan Li,Qingru Wu,Wenjun Liu,Yongjiang Ma,Danning Xu,Yugu Li

Human genomics 15:50 PubMed34362467

2021

LncRNA-TUG1 promotes the progression of infantile hemangioma by regulating miR-137/IGFBP5 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Lili Zhou,Xiao Jia,Xiangzheng Yang

International journal of molecular sciences 21: PubMed32659913

2020

Dual Leucine Zipper Kinase Is Constitutively Active in the Adult Mouse Brain and Has Both Stress-Induced and Homeostatic Functions.

Applications

Species

Mouse

Sunil Goodwani,Celia Fernandez,Paul J Acton,Virginie Buggia-Prevot,Morgan L McReynolds,Jiacheng Ma,Cheng Hui Hu,Mary E Hamby,Yongying Jiang,Kang Le,Michael J Soth,Philip Jones,William J Ray

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-IGFBP5 antibody [EPR18013-137]

Immunoprecipitation - Anti-IGFBP5 antibody [EPR18013-137] (AB254324)

Western blot - Anti-IGFBP5 antibody [EPR18013-137] (AB254324)

Western blot - Anti-IGFBP5 antibody [EPR18013-137] (AB254324)

Western blot - Anti-IGFBP5 antibody [EPR18013-137] (AB254324)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (7)

Product promise