Anti-IGFBP7 antibody [EPR11912(B)]

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGFBP7 antibody [EPR11912(B)] (AB171085)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung cancer tissue sections labeling IGFBP7 with purified ab171085 at 1 : 250 dilution (2.4 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use). PBS instead of the primary antibody was used as the negative control.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGFBP7 antibody [EPR11912(B)] (AB171085)

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling IGFBP7 with ab171085 at 1/50 dilution. This image was produced using unpurified antibody.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-IGFBP7 antibody [EPR11912(B)] (AB171085)

Immunocytochemistry/ Immunofluorescence analysis of MCF7 (human breast adenocarcinoma epithelial cell) cells labeling IGFBP7 with Purified ab171085 at 1 : 100 (6.0 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1 : 200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor^® 488 ab150077) was used as the secondary antibody at 1 : 1000 (2 μg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-IGFBP7 antibody [EPR11912(B)] (AB171085)

Intracellular flow cytometric analysis of permeabilized 293T cells labeling IGFBP7 with ab171085 at 1/250 dilution (red) compared to a rabbit IgG negative control (green). This image was produced using unpurified antibody.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-IGFBP7 antibody [EPR11912(B)] (AB171085)

Immunofluorescent analysis of MCF7 cells labeling IGFBP7 with ab171085 at 1/250 dilution. This image was produced using unpurified antibody.

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-IGFBP7 antibody [EPR11912(B)] (AB171085)

Intracellular Flow Cytometry analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling IGFBP7 with purified ab171085 at 1/60 dilution (10 ug/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor^® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGFBP7 antibody [EPR11912(B)] (AB171085)

Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling IGFBP7 with ab171085 at 1/50 dilution. This image was produced using unpurified antibody.

• WB

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Western blot - Anti-IGFBP7 antibody [EPR11912(B)] (AB171085)

All lanes:

Western blot - Anti-IGFBP7 antibody [EPR11912(B)] (ab171085) at 1/10000 dilution

All lanes:

SW480 (Human colorectal adenocarcinoma epithelial cell) whole cell lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 29 kDa

Observed band size: 29 kDa

false

• WB

Supplier Data

Western blot - Anti-IGFBP7 antibody [EPR11912(B)] (AB171085)

This image was produced using unpurified antibody.

All lanes:

Western blot - Anti-IGFBP7 antibody [EPR11912(B)] (ab171085) at 1/1000 dilution

Lane 1:

SW480 cell lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Lane 3:

MCF7 cell lysate at 10 µg

Predicted band size: 29 kDa

false