AB124055

Anti-IgG antibody [R10Z8E9]

Recombinant
Lab Essentials
What is this?

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(19 Publications)

Anti-IgG antibody [R10Z8E9] (ab124055) is a mouse monoclonal antibody detecting IgG in Western Blot, ELISA. Suitable for Human.

View Alternative Names

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma-2 chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G

2 Images

Indirect ELISA - Anti-IgG antibody [R10Z8E9] (AB124055)

I-ELISA

Lab

Indirect ELISA - Anti-IgG antibody [R10Z8E9] (AB124055)

50ng Human IgG1 (50μL at 1000ng/mL) was coated onto 96-wells. Serial dilutions (0, 0.001, 0.005, 0.020, 0.078, 0.313, 1.25, 5μg/mL) of ab124055 (50μL) were incubated with Human IgG1 for 60 minutes by shaking. This was followed by adding goat anti-mouse IgG, (H+L), phosphatase-conjugated secondary antibody 1/1000 diluted (50μL) into each well and incubating for another 40 minutes. After washing, 50 μL of PNPP was added and incubated for 13 minutes without shaking for color development. OD was read at 405nm within 5 minutes.

Lab

Western blot - Anti-IgG antibody [R10Z8E9] (AB124055)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Expected MW : 50 kDa.

All lanes:

Western blot - Anti-IgG antibody [R10Z8E9] (ab124055) at 1/1000 dilution

All lanes:

Human serum lysate at 10 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>)

Predicted band size: 36 kDa

false

Exposure time: 3min

Carrier free

Anti-IgG antibody [R10Z8E9] - BSA and Azide free

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

R10Z8E9

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

WB, I-ELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Recombinant full length protein - Human": { "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-IgG antibody [R10Z8E9] (ab124055) is a mouse recombinant monoclonal antibody and is validated for use in Western Blot (WB), ELISA in Human samples.

What is the molecular weight of IgG?
Anti-IgG [R10Z8E9] (ab124055) specifically detects a band for IgG (UniProt: P01857) at a molecular weight of 36kDa.

Other related products
We have a range of other formats of antibody clone [R10Z8E9] also available for your convenience: ab124055, Carrier free - ab256120

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information IgG

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Publications (19)

Recent publications for all applications. Explore the full list and refine your search

Open life sciences 20:20251126 PubMed40822968

2025

SATB2 promotes humeral fracture healing in rats by activating the PI3K/AKT pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Liantao Liu,Shuai Rong,Xiaobin Zhou,Hao Li,Kepei Zhen,Chong Zheng,Kewei Li

Clinics (Sao Paulo, Brazil) 80:100653 PubMed40273496

2025

circPOLA2 promotes proliferation, invasion, migration, and epithelial-mesenchymal transition in breast cancer via the miR-1224-5p/HMGA2 axis.

Applications

Unspecified application

Species

Unspecified reactive species

XinYan Xu,Jie Li,RuiJuan Li,YanFang Tan,ZhiBing Lu

European journal of histochemistry : EJH 68: PubMed39526436

2024

Activation of Hedgehog pathway by circEEF2/miR-625-5p/TRPM2 axis promotes prostate cancer cell proliferation through mitochondrial stress.

Applications

Unspecified application

Species

Unspecified reactive species

ChenHui Zhu,LiJuan Lin,ChangQing Huang,ZhiHui Wu

Discover oncology 15:401 PubMed39225900

2024

Hsa_circYARS interacts with miR-29a-3p to up-regulate IREB2 and promote laryngeal squamous cell carcinoma progression.

Applications

Unspecified application

Species

Unspecified reactive species

Zizhao Guo,Yuxia Zhao,Naicai Guo,Meng Xu,Xiaolei Wang

The Kaohsiung journal of medical sciences 40:699-709 PubMed39031804

2024

circUQCRC2 promotes asthma progression in children by activating the VEGFA/NF-κB pathway by targeting miR-381-3p.

Applications

Unspecified application

Species

Unspecified reactive species

Li-Juan Yang,Shu-Xiang Sui,Qing-Hua Zheng,Min Wang

Discover oncology 15:165 PubMed38748048

2024

circ_0006789 promotes cervical cancer development via the miR-615-5p/HSF1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Wenyu Zhou,Weiwei Song,Meisong Lu

Molecular systems biology 20:719-740 PubMed38580884

2024

Genome-wide CRISPR screens identify novel regulators of wild-type and mutant p53 stability.

Applications

Unspecified application

Species

Unspecified reactive species

YiQing Lü,Tiffany Cho,Saptaparna Mukherjee,Carmen Florencia Suarez,Nicolas S Gonzalez-Foutel,Ahmad Malik,Sebastien Martinez,Dzana Dervovic,Robin Hyunseo Oh,Ellen Langille,Khalid N Al-Zahrani,Lisa Hoeg,Zhen Yuan Lin,Ricky Tsai,Geraldine Mbamalu,Varda Rotter,Patricia Ashton-Prolla,Jason Moffat,Lucia Beatriz Chemes,Anne-Claude Gingras,Moshe Oren,Daniel Durocher,Daniel Schramek

Cartilage 16:486-494 PubMed38366389

2024

HSF1 Increases EOGT-Mediated Glycosylation of Notch1 to Promote IL-1β-Induced Inflammatory Injury of Chondrocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanchi Huang,Wenjie Pan,Huanli Bao,Xiangxiang Sun,Chao Xu,Jianbing Ma

Molecular metabolism 65:101582 PubMed36030035

2022

RIPK1 and RIPK3 regulate TNFα-induced β-cell death in concert with caspase activity.

Applications

Unspecified application

Species

Unspecified reactive species

Christopher J Contreras,Noyonika Mukherjee,Renato C S Branco,Li Lin,Meghan F Hogan,Erica P Cai,Andrew A Oberst,Steven E Kahn,Andrew T Templin

Histology and histopathology 37:899-907 PubMed35451495

2022

AADAC promotes therapeutic activity of cisplatin and imatinib against ovarian cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Haijing Wang,Disong Wang,Tingting Gu,Mengjiao Zhu,Ling Cheng,Wentao Dai

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-IgG antibody [R10Z8E9]

Indirect ELISA - Anti-IgG antibody [R10Z8E9] (AB124055)

Western blot - Anti-IgG antibody [R10Z8E9] (AB124055)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (19)

Product promise