Anti-IgG [R10Z8E9] antibody (ab256120) is a mouse monoclonal antibody that is provided in a PBS only formulation free from BSA and azide. Formulated to be conjugation-ready. It is used to detect IgG in Western Blot, ELISA. Suitable for Human samples.
- BSA, sodium azide, and glycerol-free for consistent conjugation with fluorochromes, enzymes and more
Images
![Western blot - Anti-IgG antibody [R10Z8E9] - BSA and Azide free (AB256120), expandable thumbnail](https://content.abcam.com/products/images/igg-antibody-r10z8e9-bsa-and-azide-free-ab256120--western-blot-img53534.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-IgG antibody [R10Z8E9] - BSA and Azide free (AB256120)")
![Indirect ELISA - Anti-IgG antibody [R10Z8E9] - BSA and Azide free (AB256120), expandable thumbnail](https://content.abcam.com/products/images/igg-antibody-r10z8e9-bsa-and-azide-free-ab256120--indirect-elisa-img77110.jpg/_jcr_content/renditions/webp-475.webp "Indirect ELISA - Anti-IgG antibody [R10Z8E9] - BSA and Azide free (AB256120)")
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| I-ELISA | WB | IHC-P | |
|---|---|---|---|
| Human | Expected | Tested | Not recommended |
| Recombinant full length protein - Human | Tested | Not recommended | Not recommended |
I-ELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Recombinant full length protein - Human | Dilution info - | Notes - |
Target data
Alternative names
Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma-2 chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G
Recommended products
Anti-IgG [R10Z8E9] antibody (ab256120) is a mouse monoclonal antibody that is provided in a PBS only formulation free from BSA and azide. Formulated to be conjugation-ready. It is used to detect IgG in Western Blot, ELISA. Suitable for Human samples.
- BSA, sodium azide, and glycerol-free for consistent conjugation with fluorochromes, enzymes and more
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- R10Z8E9
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
Anti-IgG antibody [R10Z8E9] - BSA and Azide free (ab256120) is a mouse recombinant monoclonal antibody and is validated for use in Western Blot (WB), ELISA in Human samples.
What is the molecular weight of IgG?
Anti-IgG [R10Z8E9] - BSA and Azide free (ab256120) specifically detects a band for IgG (UniProt: P01857) at a molecular weight of 36kDa.
Other related products
We have a range of other formats of antibody clone [R10Z8E9] also available for your convenience:
Anti-IgG antibody [R10Z8E9] ab124055, Carrier free - ab256120
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-IgG antibody [R10Z8E9] - BSA and Azide free (ab256120), expandable thumbnail](https://content.abcam.com/products/images/igg-antibody-r10z8e9-bsa-and-azide-free-ab256120--western-blot-img53534.jpg "Western blot - Anti-IgG antibody [R10Z8E9] - BSA and Azide free (ab256120)")
Western blot - Anti-IgG antibody [R10Z8E9] - BSA and Azide free (ab256120)
This image was produced using Anti-IgG antibody [R10Z8E9] ab124055, the same clone but in a different formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Expected MW: 50 kDa.
All lanes: Western blot - Anti-IgG antibody [R10Z8E9] (Anti-IgG antibody [R10Z8E9] ab124055) at 1/1000 dilution
All lanes: Human serum lysate at 10 µg
SecondaryAll lanes: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366)
Predicted band size: 36 kDa
Exposure time: 3min
![Indirect ELISA - Anti-IgG antibody [R10Z8E9] - BSA and Azide free (ab256120), expandable thumbnail](https://content.abcam.com/products/images/igg-antibody-r10z8e9-bsa-and-azide-free-ab256120--indirect-elisa-img77110.jpg "Indirect ELISA - Anti-IgG antibody [R10Z8E9] - BSA and Azide free (ab256120)")
Indirect ELISA - Anti-IgG antibody [R10Z8E9] - BSA and Azide free (ab256120)
This image was produced using Anti-IgG antibody [R10Z8E9] ab124055, the same clone but in a different formulation.
50ng Human IgG1 (50μL at 1000ng/mL) was coated onto 96-wells. Serial dilutions (0, 0.001, 0.005, 0.020, 0.078, 0.313, 1.25, 5μg/mL) of Anti-IgG antibody [R10Z8E9] ab124055 (50μL) were incubated with Human IgG1 for 60 minutes by shaking. This was followed by adding goat anti-mouse IgG, (H+L), phosphatase-conjugated secondary antibody 1/1000 diluted (50μL) into each well and incubating for another 40 minutes. After washing, 50 μL of PNPP was added and incubated for 13 minutes without shaking for color development. OD was read at 405nm within 5 minutes.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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