AB248512

Anti-IgG antibody [RIGG-69] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal IgG antibody. Carrier free. Suitable for ELISA, WB and reacts with Recombinant full length protein - Rat, Recombinant full length protein - Mouse, Human, Rat, Mouse samples. Cited in 1 publication.

6 Images

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Western blot - Anti-IgG antibody [RIGG-69] - BSA and Azide free (AB248512)

This data was developed using ab133470, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-IgG antibody [RIGG-69] (<a href='/en-us/products/primary-antibodies/igg-antibody-rigg-69-ab133470'>ab133470</a>)

Lane 1:

Rat spleen tissue lysate

Lane 2:

Human tonsil tissue lysate

Predicted band size: 36 kDa

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Lab

Western blot - Anti-IgG antibody [RIGG-69] - BSA and Azide free (AB248512)

This data was developed using ab133470, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-IgG antibody [RIGG-69] (<a href='/en-us/products/primary-antibodies/igg-antibody-rigg-69-ab133470'>ab133470</a>) at 1/150 dilution

Lane 1:

Human tonsil tissue lysate at 10 µg

Lane 2:

Human heart tissue lysate at 10 µg

Lane 3:

Mouse heart tissue lysate at 10 µg

Secondary

All lanes:

HRP anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 36 kDa

Observed band size: 50 kDa

false

Lab

Western blot - Anti-IgG antibody [RIGG-69] - BSA and Azide free (AB248512)

This data was developed using ab133470, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

purified at 1/1000 dilution

Lane 1:

Human tonsil tissue lysate at 10 µg

Lane 2:

Human heart tissue lysate at 10 µg

Lane 3:

Mouse heart tissue lysate at 10 µg

Secondary

All lanes:

HRP anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 36 kDa

Observed band size: 50 kDa

false

ELISA - Anti-IgG antibody [RIGG-69] - BSA and Azide free (AB248512)

ELISA

Lab

ELISA - Anti-IgG antibody [RIGG-69] - BSA and Azide free (AB248512)

This data was developed using ab133470, the same antibody clone in a different buffer formulation.

Antibody dose-response curve using purified ab133470. Antigen concentration of 1 μg/mL. An Alkaline Phosphatase-conjugated goat anti-rabbit IgG (H+L) (1/2500) was used as the secondary antibody.

ELISA

Lab

ELISA - Anti-IgG antibody [RIGG-69] - BSA and Azide free (AB248512)

This data was developed using ab133470, the same antibody clone in a different buffer formulation.

Direct ELISA antibody dose-response curve using ab133470 between 0-1000 ng/ml. Antigen concentration of 1000 ng/mL. An alkaline phosphatase-conjugated goat anti-rabbit IgG (H+L) (1/2500) was used as the secondary antibody.

ELISA

Lab

ELISA - Anti-IgG antibody [RIGG-69] - BSA and Azide free (AB248512)

This data was developed using ab133470, the same antibody clone in a different buffer formulation.

Antibody dose-response curve using unpurified ab133470. Antigen concentration of 1 μg/mL. An Alkaline Phosphatase-conjugated goat anti-rabbit IgG (H+L) (1/2500) was used as the secondary antibody.

Unconjugated

Anti-IgG antibody [RIGG-69]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

RIGG-69

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB, ELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Recombinant full length protein - Mouse": { "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Recombinant full length protein - Rat": { "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

ab248512 is the carrier-free version of ab133470.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information IgG

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Thoracic cancer 14:450-461 PubMed36541122

2022

EIF4A3 stabilizes the expression of lncRNA AGAP2-AS1 to activate cancer-associated fibroblasts via MyD88/NF-κb signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Qingqing Xu,Tingting Zhao,Honghao Han,Jiahao Fan,Weiping Xie

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-IgG antibody [RIGG-69] - BSA and Azide free

Western blot - Anti-IgG antibody [RIGG-69] - BSA and Azide free (AB248512)

Western blot - Anti-IgG antibody [RIGG-69] - BSA and Azide free (AB248512)

Western blot - Anti-IgG antibody [RIGG-69] - BSA and Azide free (AB248512)

ELISA - Anti-IgG antibody [RIGG-69] - BSA and Azide free (AB248512)

ELISA - Anti-IgG antibody [RIGG-69] - BSA and Azide free (AB248512)

ELISA - Anti-IgG antibody [RIGG-69] - BSA and Azide free (AB248512)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (1)

Product promise