Anti-IgG1 antibody [EPR4417]

• WB

Lab

Western blot - Anti-IgG1 antibody [EPR4417] (AB108969)

Blocking and diluting buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-IgG1 antibody [EPR4417] (ab108969) at 1/10000 dilution

Lane 1:

Human tonsil lysate at 20 µg

Lane 2:

Human spleen lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

Predicted band size: 137 kDa,21 kDa,22 kDa,36 kDa,64 kDa,67 kDa,68 kDa,75 kDa,87 kDa

Observed band size: 110 kDa,18 kDa,52 kDa,68 kDa,75 kDa,76 kDa

false

• WB

Unknown

Western blot - Anti-IgG1 antibody [EPR4417] (AB108969)

All lanes:

Western blot - Anti-IgG1 antibody [EPR4417] (ab108969) at 1/1000 dilution

Lane 1:

IgG1 at 0.01 µg

Lane 2:

IgG2 at 0.01 µg

Lane 3:

IgG3 at 0.01 µg

Lane 4:

IgG4 at 0.01 µg

Lane 5:

Human tonsil lysate at 10 µg

Lane 6:

Human spleen lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 36 kDa

false

• OI-RD Scanning

Unknown

OI-RD Scanning - Anti-IgG1 antibody [EPR4417] (AB108969)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.