AB133469

Anti-IgG1 + IgG2a + IgG3 antibody [M204-3]

Recombinant
RabMAb
Lab Essentials
What is this?

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(41 Publications)

Anti-IgG1 + IgG2a + IgG3 antibody [M204-3] (ab133469) is a rabbit monoclonal antibody detecting IgG1 + IgG2a + IgG3 in Western Blot, ELISA. Suitable for Mouse, Rat.

- Biophysical QC for unrivalled batch-batch consistency
- Over 20 publications

3 Images

Supplier Data

Western blot - Anti-IgG1 + IgG2a + IgG3 antibody [M204-3] (AB133469)

Blocking buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-IgG1 + IgG2a + IgG3 antibody [M204-3] (ab133469) at 1/10000 dilution

Lane 1:

Rat plasma at 10 µg

Lane 2:

Mouse plasma at 10 µg

Secondary

All lanes:

Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

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ELISA - Anti-IgG1 + IgG2a + IgG3 antibody [M204-3] (AB133469)

ELISA

Supplier Data

ELISA - Anti-IgG1 + IgG2a + IgG3 antibody [M204-3] (AB133469)

Antibody dose-response curve using ab133469. Antigen concentration of 1μg/ml. An Alkaline Phosphatase-conjugated goat anti-rabbit IgG (H+L) (1/2500) was used as the secondary antibody.

ELISA

Lab

ELISA - Anti-IgG1 + IgG2a + IgG3 antibody [M204-3] (AB133469)

Direct ELISA antibody dose-response curve using ab133469 between 0-1000 ng/ml. Antigen concentration of 1000 ng/mL. An alkaline phosphatase-conjugated goat anti-rabbit IgG (H+L) (1/2500) was used as the secondary antibody.

Carrier free

Anti-IgG1 + IgG2a + IgG3 antibody [M204-3] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

M204-3

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat

Applications

ELISA, WB

applications

Immunogen

This product was produced with the following immunogens:

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Rat": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Recombinant fragment - Rat": { "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "0.015-1 µg/mL", "ELISA-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-IgG1 + IgG2a + IgG3 antibody [M204-3] (ab133469) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), ELISA in Mouse, Rat samples.

Trusted by the scientific community
Anti-IgG1 + IgG2a + IgG3 [M204-3] (ab133469) was first used in a scientific publication in 2012 and has been cited over 20 times in peer-reviewed journals.

Other related products
We have a range of other formats of antibody clone [M204-3] also available for your convenience: ab133469, Carrier free - ab226129, Alexa Fluor® 647 - ab324147

Species reactivity
Human: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.09% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information IgG1

Additional targets

Mouse IgG2a,

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Publications (41)

Recent publications for all applications. Explore the full list and refine your search

PLoS pathogens 21:e1013563 PubMed41052208

2025

Dysplastic lung repair fosters a tuberculosis-promoting microenvironment through maladaptive macrophage polarization.

Applications

Unspecified application

Species

Unspecified reactive species

Shivraj M Yabaji,Suruchi Lata,Anna E Tseng,Prasanna Babu Araveti,Ming Lo,Igor Gavrish,Aoife K O'Connell,Hans P Gertje,Anna C Belkina,Colleen E Thurman,Hirofumi Kiyokawa,Darrell Kotton,Shumin Tan,Janice J Endsley,William R Bishai,Nicholas Crossland,Lester Kobzik,Igor Kramnik

Experimental hematology & oncology 14:91 PubMed40605119

2025

Bladder cancer subtypes exhibit limited plasticity across different microenvironments and in metastases.

Applications

Unspecified application

Species

Unspecified reactive species

Carina Bernardo,Subhayan Chattopadhyay,Natalie Andersson,Pontus Eriksson,Benjamin Medle,Lena Tran,Nour Al Dain Marzouka,Adam Mattsson,Aymeric Zadoroznyj,Malin Larsson,Fredrik Liedberg,Mattias Höglund,Gottfrid Sjödahl

iScience 28:111876 PubMed40060890

2025

JNJ-78306358, a first-in-class bispecific T cell engaging antibody targeting CD3 and HLA-G.

Applications

Unspecified application

Species

Unspecified reactive species

Nataša Obermajer,Adam Zwolak,Kelly van de Ven,Shana Versmissen,Krista Menard,Katharine Rogers,Ted Petley,Dan Weinstock,Jason Aligo,Jaymala Patel,Ken Tian,Lorraine Angelillo,Fang Yi,Stephen Jarantow,Keith Schutsky,Yoshitomo Hamuro,Diana Alvarez Arias,Kristel Buyens,Tsun-Wen Sheena Yao,Vince Torti,Aleksandra Brajic,Brian Geist,Marjolein van Heerden,Gerald Chu,Bie Verbist,Maté Ongenaert,Julien Häsler,Kathryn Packman,Jacintha Shenton,Laurie Lenox,Jacalyn Clawson,Regina J Brown,Josh Lauring,James G Greger,Dirk Brehmer,Sanjaya Singh,Matthew V Lorenzi,Sylvie Laquerre

Cancer medicine 14:e70615 PubMed40059728

2025

Prognostic Value of Trop-2 Expression in Nonmetastatic Triple-Negative Breast Cancer and Correlation With Emerging Biomarkers.

Applications

Unspecified application

Species

Unspecified reactive species

William Jacot,Marie-Christine Chateau,Simon Thezenas,Séverine Guiu,Nelly Firmin,Virginie Lafont,Gwendal Lazennec,Charles Theillet,Florence Boissière-Michot

Nature communications 16:2036 PubMed40021625

2025

Bone morphogenetic protein (BMP) signaling determines neuroblastoma cell fate and sensitivity to retinoic acid.

Applications

Unspecified application

Species

Unspecified reactive species

Min Pan,Yinwen Zhang,William C Wright,Xueying Liu,Barbara Passaia,Duane Currier,Jonathan Low,Richard H Chapple,Jacob A Steele,Jon P Connelly,Bensheng Ju,Emily Plyler,Meifen Lu,Allister J Loughran,Lei Yang,Brian J Abraham,Shondra M Pruett-Miller,Burgess Freeman,George E Campbell,Michael A Dyer,Taosheng Chen,Elizabeth Stewart,Selene Koo,Heather Sheppard,John Easton,Paul Geeleher

STAR protocols 6:103640 PubMed39982826

2025

Protocol for 3D multiplexed fluorescent imaging of pulmonary TB lesions using Opal-TSA dyes for signal amplification.

Applications

Unspecified application

Species

Unspecified reactive species

Suruchi Lata,Shivraj M Yabaji,Aoife K O'Connell,Hans P Gertje,Michael T Kirber,Nicholas A Crossland,Igor Kramnik

Cancer research 85:424-441 PubMed39531507

2024

Conditional Activation of c-MYC in Distinct Catecholaminergic Cells Drives Development of Neuroblastoma or Somatostatinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Tingting Wang,Lingling Liu,Jie Fang,Hongjian Jin,Sivaraman Natarajan,Heather Sheppard,Meifen Lu,Gregory Turner,Thomas Confer,Melissa Johnson,Jeffrey Steinberg,Larry Ha,Nour Yadak,Richa Jain,David J Picketts,Xiaotu Ma,Andrew Murphy,Andrew M Davidoff,Evan S Glazer,John Easton,Xiang Chen,Ruoning Wang,Jun Yang

The EMBO journal 43:5972-6000 PubMed39448884

2024

Cyclophilin D plays a critical role in the survival of senescent cells.

Applications

Unspecified application

Species

Unspecified reactive species

Margherita Protasoni,Vanessa López-Polo,Camille Stephan-Otto Attolini,Julian Brandariz,Nicolas Herranz,Joaquin Mateo,Sergio Ruiz,Oscar Fernandez-Capetillo,Marta Kovatcheva,Manuel Serrano

The American journal of pathology 194:1162-1170 PubMed38880601

2024

Identification of HTRA4 as a Transcriptional Target of p63 in Trophoblast.

Applications

Unspecified application

Species

Unspecified reactive species

Mary E Donohoe,Robert Morey,Yingchun Li,Donald Pizzo,Sampada Kallol,Hee-Young Cho,Francesca Soncin,Mana M Parast

Nature cancer 5:448-462 PubMed38267628

2024

The efficacy of chemotherapy is limited by intratumoral senescent cells expressing PD-L2.

Applications

Unspecified application

Species

Unspecified reactive species

Selim Chaib,José Alberto López-Domínguez,Marta Lalinde-Gutiérrez,Neus Prats,Ines Marin,Olga Boix,Andrea García-Garijo,Kathleen Meyer,María Isabel Muñoz,Mònica Aguilera,Lidia Mateo,Camille Stephan-Otto Attolini,Susana Llanos,Sandra Pérez-Ramos,Marta Escorihuela,Fatima Al-Shahrour,Timothy P Cash,Tamara Tchkonia,James L Kirkland,María Abad,Alena Gros,Joaquín Arribas,Manuel Serrano

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-IgG1 + IgG2a + IgG3 antibody [M204-3]

Western blot - Anti-IgG1 + IgG2a + IgG3 antibody [M204-3] (AB133469)

ELISA - Anti-IgG1 + IgG2a + IgG3 antibody [M204-3] (AB133469)

ELISA - Anti-IgG1 + IgG2a + IgG3 antibody [M204-3] (AB133469)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Additional targets

Publications (41)

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