Skip to main content

Rabbit Recombinant Monoclonal IGHG2 antibody. Suitable for IHC-P, ELISA, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 4 publications.

Be the first to review this product! Submit a review

Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgG2 antibody [EPR4418] (AB134050), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-IgG2 antibody [EPR4418] (AB134050), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-IgG2 antibody [EPR4418] (AB134050), expandable thumbnail
  • Western blot - Anti-IgG2 antibody [EPR4418] (AB134050), expandable thumbnail
  • Western blot - Anti-IgG2 antibody [EPR4418] (AB134050), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPELISAWBICC/IFFlow Cyt (Intra)
Human
Tested
Not recommended
Expected
Tested
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/40000
Notes

For unpurified use at 1/500-1/1000

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/5000
Notes

For unpurified use at 1/10000-1/20000

Tested
Tested

Species
Human
Dilution info
1/50 - 1/100
Notes

-

Tested
Tested

Species
Human
Dilution info
1/70
Notes

-

Target data

Function

Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:20176268, PubMed:22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed:17576170, PubMed:20176268).

Alternative names

Immunoglobulin heavy constant gamma 2, Ig gamma-2 chain C region, Ig gamma-2 chain C region DOT, Ig gamma-2 chain C region TIL, Ig gamma-2 chain C region ZIE, IGHG2

Recommended products

Rabbit Recombinant Monoclonal IGHG2 antibody. Suitable for IHC-P, ELISA, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 4 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR4418
Purification technique
Affinity purification Protein A
Specificity

ab134050 does not cross-react with human IgG1, IgG3 or IgG4.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C

Notes

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgG2 antibody [EPR4418] (ab134050), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgG2 antibody [EPR4418] (ab134050)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue sections labeling IgG2 with purified ab134050 at 1:40000 dilution (0.025 µg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Flow Cytometry (Intracellular) - Anti-IgG2 antibody [EPR4418] (ab134050), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-IgG2 antibody [EPR4418] (ab134050)

    Intracellular Flow Cytometry analysis of Raji (human Burkitt's lymphoma cell line) cells labeling IgG2 with purified ab134050 at 1/100 dilution (10μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Immunocytochemistry/ Immunofluorescence - Anti-IgG2 antibody [EPR4418] (ab134050), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-IgG2 antibody [EPR4418] (ab134050)

    Immunocytochemistry/ Immunofluorescence analysis of Raji (human Burkitt's lymphoma cell line) cells labeling IgG2 with purified ab134050 at 1:100 dilution (10 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Western blot - Anti-IgG2 antibody [EPR4418] (ab134050), expandable thumbnail

    Western blot - Anti-IgG2 antibody [EPR4418] (ab134050)

    All lanes: Western blot - Anti-IgG2 antibody [EPR4418] (ab134050) at 1/5000 dilution

    Lane 1: Human spleen lysates at 15 µg

    Lane 2: Human tonsil lysates at 15 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 36 kDa

    Observed band size: 52 kDa

  • Western blot - Anti-IgG2 antibody [EPR4418] (ab134050), expandable thumbnail

    Western blot - Anti-IgG2 antibody [EPR4418] (ab134050)

    All lanes: Western blot - Anti-IgG2 antibody [EPR4418] (ab134050) at 1/10000 dilution

    Lane 1: Human Recombinant IgG2 at 0.01 µg

    Lane 2: Human Recombinant IgG1 at 0.01 µg

    Lane 3: Human Recombinant IgG3 at 0.01 µg

    Lane 4: Human Recombinant IgG4 at 0.01 µg

    Lane 5: Human spleen lysate at 10 µg

    Lane 6: Human tonsil lysate at 10 µg

    Secondary

    All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 36 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgG2 antibody [EPR4418] (ab134050), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgG2 antibody [EPR4418] (ab134050)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling IgG2 with unpurified ab134050 at 1/500 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgG2 antibody [EPR4418] (ab134050), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgG2 antibody [EPR4418] (ab134050)

    Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue labeling IgG2 with unpurified ab134050 at 1/500 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com