Anti-IgG2 antibody [EPR4418]

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-IgG2 antibody [EPR4418] (AB134050)

Immunocytochemistry/ Immunofluorescence analysis of Raji (human Burkitt's lymphoma cell line) cells labeling IgG2 with purified ab134050 at 1 : 100 dilution (10 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1 : 200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1 : 1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-IgG2 antibody [EPR4418] (AB134050)

Intracellular Flow Cytometry analysis of Raji (human Burkitt's lymphoma cell line) cells labeling IgG2 with purified ab134050 at 1/100 dilution (10μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgG2 antibody [EPR4418] (AB134050)

Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue labeling IgG2 with unpurified ab134050 at 1/500 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgG2 antibody [EPR4418] (AB134050)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling IgG2 with unpurified ab134050 at 1/500 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgG2 antibody [EPR4418] (AB134050)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue sections labeling IgG2 with purified ab134050 at 1 : 40000 dilution (0.025 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

• WB

Lab

Western blot - Anti-IgG2 antibody [EPR4418] (AB134050)

All lanes:

Western blot - Anti-IgG2 antibody [EPR4418] (ab134050) at 1/5000 dilution

Lane 1:

Human spleen lysates at 15 µg

Lane 2:

Human tonsil lysates at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 36 kDa

Observed band size: 52 kDa

false

• WB

Unknown

Western blot - Anti-IgG2 antibody [EPR4418] (AB134050)

All lanes:

Western blot - Anti-IgG2 antibody [EPR4418] (ab134050) at 1/10000 dilution

Lane 1:

Human Recombinant IgG2 at 0.01 µg

Lane 2:

Human Recombinant IgG1 at 0.01 µg

Lane 3:

Human Recombinant IgG3 at 0.01 µg

Lane 4:

Human Recombinant IgG4 at 0.01 µg

Lane 5:

Human spleen lysate at 10 µg

Lane 6:

Human tonsil lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 36 kDa

false