Rabbit Recombinant Monoclonal IGHG4 antibody. Suitable for IHC-P, WB and reacts with Human, Recombinant fragment - Human samples. Cited in 22 publications.
Images
![Western blot - Anti-IgG4 antibody [EP4420] (AB109493), expandable thumbnail](https://content.abcam.com/products/images/igg4-antibody-ep4420-ab109493--western-blot-img167160.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-IgG4 antibody [EP4420] (AB109493)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgG4 antibody [EP4420] (AB109493), expandable thumbnail](https://content.abcam.com/products/images/igg4-antibody-ep4420-ab109493--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img172750.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgG4 antibody [EP4420] (AB109493)")
![Western blot - Anti-IgG4 antibody [EP4420] (AB109493), expandable thumbnail](https://content.abcam.com/products/images/igg4-antibody-ep4420-ab109493--western-blot-img132915.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-IgG4 antibody [EP4420] (AB109493)")
Publications
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- ImmunoHorizons 7:366-3792023SRF617 Is a Potent Inhibitor of CD39 with Immunomodulatory and Antitumor Properties.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMichael C Warren,Stephan Matissek,Matthew Rausch,Marisella Panduro,R J Hall,Austin Dulak,David Brennan,Sonia Das Yekkirala,Secil Koseoglu,Ricard Masia,Yu Yang,Navamallika Reddy,Robert Prenovitz,Jamie Strand,Tauqueer Zaidi,Erik Devereaux,Célia Jacoberger Foissac,John Stagg,Benjamin H Lee,Pamela Holland,Vito J Palombella,Andrew C LakePubMed 37219538
- Journal of clinical medicine 11:2022Esophageal Mucosal Permeability as a Surrogate Measure of Cure in Eosinophilic Esophagitis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJunji Chen,Tadayuki Oshima,Xinyi Huang,Toshihiko Tomita,Hirokazu Fukui,Hiroto MiwaPubMed 35888006
- Journal of translational medicine 20:1122022Tumor-B-cell interactions promote isotype switching to an immunosuppressive IgG4 antibody response through upregulation of IL-10 in triple negative breast cancers.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNicole J Toney,Lynn M Opdenaker,Kader Cicek,Lisa Frerichs,Christopher Ryan Kennington,Samuel Oberly,Holly Archinal,Rajasekharan Somasundaram,Jennifer Sims-MourtadaPubMed 35255925
- Digestive diseases and sciences 67:3639-36482021Gastrointestinal IgG4 Deposition Is a New Histopathological Feature of Eosinophilic Gastroenteritis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSatoshi Kosaka,Fumio Tanaka,Akinobu Nakata,Yuji Nadatani,Shusei Fukunaga,Koji Otani,Shuhei Hosomi,Noriko Kamata,Yasuaki Nagami,Koichi Taira,Toshio Watanabe,Yasuhiro FujiwaraPubMed 34499268
- medRxiv : the preprint server for health sciences :2021Next-generation Serology by Mass Spectrometry: Readout of the SARS-CoV-2 Antibody Repertoire.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRafael D Melani,Benjamin J Des Soye,Jared O Kafader,Eleonora Forte,Michael Hollas,Voislav Blagojevic,Fernanda Negrão,John P McGee,Bryon Drown,Cameron Lloyd-Jones,Henrique S Seckler,Jeannie M Camarillo,Philip D Compton,Richard D LeDuc,Bryan Early,Ryan T Fellers,Byoung-Kyu Cho,Basil Baby Mattamana,Young Ah Goo,Paul M Thomas,Michelle K Ash,Pavan P Bhimalli,Lena Al-Harthi,Beverly E Sha,Jeffrey R Schneider,Neil L KelleherPubMed 34268518
- Molecular medicine reports 23:2021Expression of immunoglobulin G in human proximal tubular epithelial cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhenling Deng,Ziyang Jing,Yanhong Guo,Junfan Ma,Huige Yan,Zhan Shi,Hui Deng,Yaoxian Liang,Song Wang,Zhuan Cui,Yuejuan Pan,Xiaoyan Qiu,Yue WangPubMed 33760139
- Blood cancer discovery 1:146-1542020Defining an Optimal Dual-Targeted CAR T-cell Therapy Approach Simultaneously Targeting BCMA and GPRC5D to Prevent BCMA Escape-Driven Relapse in Multiple Myeloma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCarlos Fernández de Larrea,Mette Staehr,Andrea V Lopez,Khong Y Ng,Yunxin Chen,William D Godfrey,Terence J Purdon,Vladimir Ponomarev,Hans-Guido Wendel,Renier J Brentjens,Eric L SmithPubMed 33089218
- The Journal of allergy and clinical immunology 145:968-981.e142019B lymphocytes directly contribute to tissue fibrosis in patients with IgG-related disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEmanuel Della-Torre,Elena Rigamonti,Cory Perugino,Simona Baghai-Sain,Na Sun,Naoki Kaneko,Takashi Maehara,Lucrezia Rovati,Maurilio Ponzoni,Raffaella Milani,Marco Lanzillotta,Vinay Mahajan,Hamid Mattoo,Ivan Molineris,Vikram Deshpande,John H Stone,Massimo Falconi,Angelo A Manfredi,Shiv PillaiPubMed 31319101
- Frontiers in immunology 10:6932019Clinical and Prognostic Significance of Serum IgG4 in Chronic Periaortitis. An Analysis of 113 Patients.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFederica Maritati et. al.PubMed 31019511
- Life science alliance 1:2018The expansion in lymphoid organs of IL-4 BATF T follicular helper cells is linked to IgG4 class switching in vivo.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTakashi Maehara et. al.PubMed 29984361
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Expected |
| Recombinant fragment - Human | Not recommended | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/5000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info 1/1000 | Notes For unpurified, use at 1/1000 - 1/10000. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
1 product for Alternative Version
Target data
Function
Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:20176268, PubMed:22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed:17576170, PubMed:20176268).
Alternative names
Immunoglobulin heavy constant gamma 4, Ig gamma-4 chain C region, IGHG4
Recommended products
Rabbit Recombinant Monoclonal IGHG4 antibody. Suitable for IHC-P, WB and reacts with Human, Recombinant fragment - Human samples. Cited in 22 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EP4420
- Purification technique
- Affinity purification Protein A
- Specificity
Does not cross-react with IgG1, IgG2, or IgG3.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Stable for 12 months at -20°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-IgG4 antibody [EP4420] (ab109493), expandable thumbnail](https://content.abcam.com/products/images/igg4-antibody-ep4420-ab109493--western-blot-img167160.jpg "Western blot - Anti-IgG4 antibody [EP4420] (ab109493)")
Western blot - Anti-IgG4 antibody [EP4420] (ab109493)
All lanes: Western blot - Anti-IgG4 antibody [EP4420] (ab109493) at 1/1000 dilution
Lane 1: Human spleen lysate at 15 µg
Lane 2: Human plasma lysate at 15 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 36 kDa
Observed band size: 52 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgG4 antibody [EP4420] (ab109493), expandable thumbnail](https://content.abcam.com/products/images/igg4-antibody-ep4420-ab109493--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img172750.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgG4 antibody [EP4420] (ab109493)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgG4 antibody [EP4420] (ab109493)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue sections labeling IgG4 with Purified ab109493 at 1:4000 dilution (0.023 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.
![Western blot - Anti-IgG4 antibody [EP4420] (ab109493), expandable thumbnail](https://content.abcam.com/products/images/igg4-antibody-ep4420-ab109493--western-blot-img132915.jpg "Western blot - Anti-IgG4 antibody [EP4420] (ab109493)")
Western blot - Anti-IgG4 antibody [EP4420] (ab109493)
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 180 seconds.
All lanes: Western blot - Anti-IgG4 antibody [EP4420] (ab109493) at 1/1000 dilution
Lane 1: IgG4 Recombinant protein at 0.005 µg
Lane 2: IgG1 Recombinant protein at 0.01 µg
Lane 3: IgG2 Recombinant protein at 0.01 µg
Lane 4: IgG3 Recombinant protein at 0.01 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 36 kDa
Observed band size: 52 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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