Anti-IGJ antibody [EPR23130-113] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGJ antibody [EPR23130-113] - BSA and Azide free (AB269860)

Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue labeling IGJ with ab269855 at 1/8000 dilution (0.06 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in plasma cells of human bladder cancer is observed. The section was incubated with ab269855 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab269855).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGJ antibody [EPR23130-113] - BSA and Azide free (AB269860)

Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling IGJ with ab269855 at 1/8000 dilution (0.06 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in plasma cells of human spleen (PMID : 12389099) is observed. The section was incubated with ab269855 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab269855).

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-IGJ antibody [EPR23130-113] - BSA and Azide free (AB269860)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab269855).

ab269855 staining IGJ in Daudi cells, with negative expression in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilised with 0.1% Triton x-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab269855 at 5 μg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin at 0.5 μg/ml. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor^® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150119, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor^® 647), pre-adsorbed at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.

This product also work with 100% methanol (5 min) fixation under the same testing conditions.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGJ antibody [EPR23130-113] - BSA and Azide free (AB269860)

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling IGJ with ab269855 at 1/8000 dilution (0.06 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in plasma cells of human colon (PMID : 12389099) is observed. The section was incubated with ab269855 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab269855).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-IGJ antibody [EPR23130-113] - BSA and Azide free (AB269860)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Daudi cells labeling IGJ with ab224687 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in Daudi cells is observed. ab195889 Anti-alpha Tubulin antibody (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab269855).

• IP

Supplier Data

Immunoprecipitation - Anti-IGJ antibody [EPR23130-113] - BSA and Azide free (AB269860)

IGJ was immunoprecipitated from 0.35 mg human tonsil lysate with ab269855 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using 269855 1/1000 dilution (0.48 μg/ml). VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : Human tonsil lysate 10 μg
Lane 2 : ab269855 IP in human tonsil lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab269855 in human tonsil lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 3 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab269855).

All lanes:

Immunoprecipitation - Anti-IGJ antibody [EPR23130-113] (<a href='/en-us/products/primary-antibodies/igj-antibody-epr23130-113-ab269855'>ab269855</a>)

Predicted band size: 18 kDa

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