Rabbit Recombinant Monoclonal IGJ antibody. Suitable for Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 1 publication.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGJ antibody [SP105] (AB105229), expandable thumbnail](https://content.abcam.com/products/images/igj-antibody-sp105-ab105229--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img49551.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGJ antibody [SP105] (AB105229)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGJ antibody [SP105] (AB105229), expandable thumbnail](https://content.abcam.com/products/images/igj-antibody-sp105-ab105229--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img40308.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGJ antibody [SP105] (AB105229)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGJ antibody [SP105] (AB105229), expandable thumbnail](https://content.abcam.com/products/images/igj-antibody-sp105-ab105229--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img133759.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGJ antibody [SP105] (AB105229)")
![Flow Cytometry (Intracellular) - Anti-IGJ antibody [SP105] (AB105229), expandable thumbnail](https://content.abcam.com/products/images/igj-antibody-sp105-ab105229--flow-cytometry-intracellular-img414236.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-IGJ antibody [SP105] (AB105229)")
Publications
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- International journal of oncology 63:2023IGJ suppresses breast cancer growth and metastasis by inhibiting EMT via the NF‑κB signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMengxue Wang,Yushen Wu,Xunjia Li,Meng Dai,Shengwei LiPubMed 37539706
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Flow Cyt (Intra) | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes Perform antigen retrieval before commencing with IHC staining protocol by boiling tissue section in 10 mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Function
Serves to link two monomer units of either IgM or IgA. In the case of IgM, the J chain-joined dimer is a nucleating unit for the IgM pentamer, and in the case of IgA it induces dimers and/or larger polymers. It also helps to bind these immunoglobulins to secretory component.
Alternative names
IGCJ, IGJ, JCHAIN, Immunoglobulin J chain, Joining chain of multimeric IgA and IgM
Recommended products
Rabbit Recombinant Monoclonal IGJ antibody. Suitable for Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 1 publication.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- SP105
- Purification technique
- Affinity purification Protein A/G
- Concentration
- Purification notes
Purified from TCS by protein A/G.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGJ antibody [SP105] (ab105229), expandable thumbnail](https://content.abcam.com/products/images/igj-antibody-sp105-ab105229--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img49551.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGJ antibody [SP105] (ab105229)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGJ antibody [SP105] (ab105229)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human stomach tissue sections labeling IGJ with ab105229 at 1/100 dilution (1.06 μg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the stromal cells of human stomach, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab105229 for 10 mins at room temperature.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGJ antibody [SP105] (ab105229), expandable thumbnail](https://content.abcam.com/products/images/igj-antibody-sp105-ab105229--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img40308.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGJ antibody [SP105] (ab105229)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGJ antibody [SP105] (ab105229)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling IGJ with ab105229 at 1/100 dilution (1.06 μg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human tonsil, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab105229 for 10 mins at room temperature.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGJ antibody [SP105] (ab105229), expandable thumbnail](https://content.abcam.com/products/images/igj-antibody-sp105-ab105229--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img133759.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGJ antibody [SP105] (ab105229)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGJ antibody [SP105] (ab105229)
ab105229, at 1/100 dilution, staining IGJ in formalin-fixed, paraffin-embedded Human tonsil tissue by Immunohistochemistry.
![Flow Cytometry (Intracellular) - Anti-IGJ antibody [SP105] (ab105229), expandable thumbnail](https://content.abcam.com/products/images/igj-antibody-sp105-ab105229--flow-cytometry-intracellular-img414236.jpg "Flow Cytometry (Intracellular) - Anti-IGJ antibody [SP105] (ab105229)")
Flow Cytometry (Intracellular) - Anti-IGJ antibody [SP105] (ab105229)
Flow cytometry analysis of Daudi (Human Burkitt's lymphoma lymphoblast) labeling IGJ with purified ab105229 at 1/20 dilution (5.3 μg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Black. Unlableled control -Unlabelled cells / blue.
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Product protocols
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com