AB18201

Anti-IgM antibody [UCH-B1]

Name: Anti-IgM antibody [UCH-B1] (ab18201) | Abcam
Brand: Abcam Limited
SKU: AB18201
Price: 500 USD
Availability: InStock

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(4 Publications)

Mouse Monoclonal IgM antibody. Suitable for Flow Cyt, ICC/IF, IHC-Fr and reacts with Human samples. Cited in 4 publications.

View Alternative Names

Constant region of heavy chain of IgM, DKFZp686I15196, DKFZp686I15212, FLJ00385, Hepatitis B virus receptor binding protein, Ig mu chain C region, IgM heavy chain constant region, Immunoglobulin heavy constant mu, Immunoglobulin mu chain, Imunoglobulin heavy chain, Imunoglobulin heavy chain constant region mu, Imunoglobulin heavy chain mu constant region, MGC104996, MGC52291, VH

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

UCH-B1

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

ICC/IF, Flow Cyt, IHC-Fr

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "Acetone fixed." } } }

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Product details

UCH-B1 antigen (85kD) is detected on a sub-population of B lymphocytes, probably related to stage of activation or maturation. UCH-B1 may be useful for differential diagnosis of B cell leukaemias and as a marker of prolymphocytic leukaemia.

Does not work on formalin fixed and parrafin embedded tissues.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A/G

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IgM also known as immunoglobulin M is a type of antibody with a molecular mass of approximately 970 kDa. It is the largest antibody present in the human circulatory system mostly found in plasma and lymphatic tissue. IgM is secreted primarily by B cells as a pentamer with a J chain providing a high avidity for antigens. When bound to an antigen IgM can activate the complement system a part of the immune system that helps clear pathogens from an organism.

Biological function summary

IgM acts as the first line of defense during the initial stages of an immune response. It plays an important part in agglutination and neutralization of pathogens forming a complex that alerts other components of the immune system to take action. IgM exists before class-switching to IgG providing immediate protection. It also plays a role in antibody-dependent cellular cytotoxicity. Although not typically a long-term solution its presence is critical for providing initial protection until more specific antibodies like IgG and IgA are produced.

Pathways

The immune response that involves IgM begins with the activation of the classical complement pathway. This pathway bridges the innate and adaptive immunity ensuring the communication and coordination of immune responses. The pathway involves C1q protein binding to IgM-antigen complexes. IgM also interacts with antigen-presenting cells facilitating the processing of antigens and interaction with T cells. These interactions connect IgM with other proteins like IgG and IgA influencing various immune response pathways.

IgM is associated with conditions such as Waldenström's macroglobulinemia and rheumatoid arthritis. Waldenström's macroglobulinemia involves the overproduction of IgM by clonal B cells leading to symptoms like anemia bleeding and neuropathy. Rheumatoid arthritis shows increased IgM levels as part of the autoimmune response affecting joints. In these diseases the IgM antibody interacts with rheumatoid factor and other immune proteins involved in inflammation demonstrating its significant role in autoimmunity and abnormal immune responses.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information IgM

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

The Journal of experimental medicine 181:161-8 PubMed7807000

1995

Molecular mechanisms that control expression of the B lymphocyte antigen receptor complex.

Applications

Unspecified application

Species

Unspecified reactive species

S A Grupp,R N Mitchell,K L Schreiber,D J McKean,A K Abbas

Chemical immunology 59:156-72 PubMed7945925

1994

Structure and function of the B-cell antigen receptor.

Applications

Unspecified application

Species

Unspecified reactive species

A L DeFranco,J H Blum,T L Stevens,D A Law,V W Chan,S P Foy,S K Datta,L Matsuuchi

Clinical and experimental immunology 82:181-7 PubMed2208792

1990

Characterization of two monoclonal antibodies (UCL4D12 and UCL3D3) that discriminate between human mantle zone and marginal zone B cells.

Applications

Unspecified application

Species

Unspecified reactive species

J Smith-Ravin,J Spencer,P C Beverley,P G Isaacson

European journal of immunology 18:67-76 PubMed3257923

1988

A new antigen identified by the monoclonal antibody UCHB 1 delivers a costimulatory signal to a subset of human B cells.

Applications

Unspecified application

Species

Unspecified reactive species

R J Armitage,D J Rowe,P C Beverly

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-IgM antibody [UCH-B1]

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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