Rabbit Polyclonal IHH antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 46 publications.
Images
Publications
Filter by
- International journal of molecular sciences 24:2023Cranial Neural Crest Specific Deletion of (TNAP) via P0-Cre Causes Abnormal Chondrocyte Maturation and Deficient Cranial Base Growth.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNaoto Ohkura,Hwa Kyung Nam,Fei Liu,Nan HatchPubMed 37895082
- Cell death & disease 14:4472023Loss of function mutation in Ank causes aberrant mineralization and acquisition of osteoblast-like-phenotype by the cells of the intervertebral disc.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTakashi Ohnishi,Victoria Tran,Kimheak Sao,Pranay Ramteke,William Querido,Ruteja A Barve,Koen van de Wetering,Makarand V RisbudPubMed 37468461
- Journal of clinical medicine 12:2023GLI1 Deficiency Impairs the Tendon-Bone Healing after Anterior Cruciate Ligament Reconstruction: In Vivo Study Using -Transgenic Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYake Liu,Shaohua Liu,Zhe Song,Daoyun Chen,Zoe Album,Samuel Green,Xianghua Deng,Scott A RodeoPubMed 36769647
- Cells 11:2022CRC Therapy Identifies Indian Hedgehog Signaling in Mouse Endometrial Epithelial Cells and Inhibition of Ihh-KLF9 as a Novel Strategy for Treating IUA.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXinhao Zhou,Yiyi Kang,Yuntzu Chang,Siyu Xia,Ming Wu,Jun Liu,Dirong Dong,Wei Zhang,Hong Chen,Hui LiPubMed 36552817
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology 36:e222582022Blocking chondrocyte hypertrophy in conditional Evc knockout mice does not modify cartilage damage in osteoarthritis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAna Lamuedra,Paula Gratal,Lucía Calatrava,Víctor Luis Ruiz-Perez,Adrián Palencia-Campos,Sergio Portal-Núñez,Aránzazu Mediero,Gabriel Herrero-Beaumont,Raquel LargoPubMed 35334131
- Stem cell reports 17:820-8342022Epithelial-derived factors induce muscularis mucosa of human induced pluripotent stem cell-derived gastric organoids.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKeiichiro Uehara,Michiyo Koyanagi-Aoi,Takahiro Koide,Tomoo Itoh,Takashi AoiPubMed 35245440
- International journal of biological sciences 18:956-9692022A Sonic Hedgehog-Gli-Bmi1 signaling pathway plays a critical role in p27 deficiency induced bone anabolism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJun Wu,Rong Wang,Xuechun Kan,Jinghan Zhang,Wen Sun,David Goltzman,Dengshun MiaoPubMed 35173529
- Proceedings of the National Academy of Sciences of the United States of America 118:2021Localized chondro-ossification underlies joint dysfunction and motor deficits in the mouse model of osteogenesis imperfecta.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJoohyun Lim,Caressa Lietman,Matthew W Grol,Alexis Castellon,Brian Dawson,Mary Adeyeye,Jyoti Rai,MaryAnn Weis,Douglas R Keene,Ronen Schweitzer,Dongsu Park,David R Eyre,Deborah Krakow,Brendan H LeePubMed 34161280
- International journal of molecular medicine 46:1993-20062020Hsa_circularRNA_0079201 suppresses chondrocyte proliferation and endochondral ossification by regulating the microRNA‑140‑3p/SMAD2 signaling pathway in idiopathic short stature.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXijuan Liu et. al.PubMed 33125098
- Molecular medicine reports 22:1119-11282020Cyclic stretch promotes the ossification of ligamentum flavum by modulating the Indian hedgehog signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRui Gao et. al.PubMed 32626952
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Consider this alternative
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Tested | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes - |
Species Human | Dilution info 1 µg/mL | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Indian hedgehog protein. The C-terminal part of the indian hedgehog protein precursor displays an autoproteolysis and a cholesterol transferase activity (By similarity). Both activities result in the cleavage of the full-length protein into two parts followed by the covalent attachment of a cholesterol moiety to the C-terminal of the newly generated N-product (By similarity). Both activities occur in the reticulum endoplasmic (By similarity). Plays a role in hedgehog paracrine signaling. Associated with the very-low-density lipoprotein (VLDL) particles to function as a circulating morphogen for endothelial cell integrity maintenance (By similarity). Indian hedgehog protein N-product. The dually lipidated indian hedgehog protein N-product is a morphogen which is essential for a variety of patterning events during development. Binds to the patched (PTCH1) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes (By similarity). Plays a role in morphogenesis of the skeleton by coordinating growth and differentiation of the endochondral skeleton (PubMed:10465785, PubMed:10631175). Positively regulates PTHLH during endochondral bone formation preventing chondrocyte hypertrophy. In contrast, Ihh is necessary for normal chondrocyte proliferation in a PTHLH-independent pathway (PubMed:10631175).
Alternative names
Indian hedgehog protein, IHH, HHG-2, Ihh
Recommended products
Rabbit Polyclonal IHH antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 46 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Specificity
Replenishment batches of our polyclonal antibody, ab39634 are tested in IHC-P. Previous batches were additionally validated in ICC/IF and WB. These applications are still expected to work and are covered by our Abpromise guarantee. You may also be interested in our alternative recombinant antibody, ab52919.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Indian hedgehog (Ihh) protein known by its gene symbol IHH plays key roles in cell signaling. Ihh with an approximate mass of 45 kDa belongs to the Hedgehog family of signaling molecules. It is expressed in various tissues including cartilage bone and parts of the gastrointestinal tract where it regulates growth and patterning. Ihh acts by binding to its receptor Patched triggering downstream signaling that influences cell fate decisions.
- Biological function summary
Ihh is significant in regulating the differentiation and proliferation of chondrocytes in the growth plate of long bones. It acts as part of a complex regulatory network coordinating with Parathyroid hormone-related protein (PTHrP) to maintain proper development of endochondral bones. Ihh also influences epithelial and mesenchymal interactions during embryo development evidencing its important role in developmental biology.
- Pathways
Ihh is a critical component of the Hedgehog signaling pathway a conserved pathway involved in embryonic development and adult tissue homeostasis. This pathway also includes proteins like Sonic hedgehog (Shh) and Desert hedgehog (Dhh) as well as the receptor Smoothened which mediates the signaling cascade. Ihh signaling impacts pathways such as the Wnt pathway influencing the proliferation and differentiation of cells thereby highlighting its integration into broader cellular signaling networks.
- Associated diseases and disorders
Ihh misregulation can lead to skeletal disorders like brachydactyly type A1 characterized by shortened fingers and toes. Furthermore abnormal Ihh signaling can contribute to certain cancers such as chondrosarcoma where overactive Hedgehog signaling promotes tumorigenesis. The relationship between Ihh and other Hedgehog proteins including Shh is key in understanding these pathologies as they share pathways that are often disrupted in disease scenarios.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
This image is courtesy of a customer review submitted by Antibody Solutions Ltd.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ihh antibody (ab39634)
ab39634 (1/50) staining Ihh in paraffin-embbeded mouse pancreas tissue sections. Tissue underwent fixation in formaldehyde, heat-mediated antigen retrieval in citrate buffer pH 6.0 and blocking (5 minutes/peroxidase block and 10 minutes/protein block). For further experimental details please refer to abreview. Strongest staining observed in islet cells of the pancreas.
Western blot - Anti-Ihh antibody (ab39634)
All lanes: Western blot - Anti-Ihh antibody (ab39634) at 1 µg/mL
All lanes: Lung (Human) Whole Cell Lysate - fetal normal tissue (ab30282) at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 45 kDa
Observed band size: 45 kDa
Exposure time: 30s
Western blot - Anti-Ihh antibody (ab39634)
The Ihh protein has a predicted molecular weight of 45 kDa. The first 27 amino acids of the Ihh sequence act as a signal sequence, and when cleaved the protein has an expected molecular weight of 42 kDa.
All lanes: Western blot - Anti-Ihh antibody (ab39634) at 1 µg/mL
Lane 1: Brain (Mouse) Tissue Lysate at 10 µg
Lane 2: Western blot - Mouse colon tissue lysate - total protein (Mouse colon tissue lysate - total protein ab29544) at 10 µg
Lane 3: Western blot - NIH/3T3 whole cell lysate (NIH/3T3 whole cell lysate ab7179) at 10 µg
SecondaryAll lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 45 kDa
Observed band size: 42 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ihh antibody (ab39634)
IHC image of Ihh staining in human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab39634, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunocytochemistry/ Immunofluorescence - Anti-Ihh antibody (ab39634)
ICC/IF image of ab39634 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab39634, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) HepG2 cells at 5µg/ml.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com