Anti-Ihh antibody [EP1192Y] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ihh antibody [EP1192Y] - BSA and Azide free (AB239853)

The paraffin-embedded sections were Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins and labeling ab52919 at 1/200 dilutions for 30 mins at room temperature and followed Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). The immunostaining was performed on a Leica Biosystems BOND^© RX instrument. Counterstained with hematoxylin. Positive staining on the Human liver. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52919).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ihh antibody [EP1192Y] - BSA and Azide free (AB239853)

The paraffin-embedded sections were Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins and labeling ab52919 at 1/1000 dilutions for 30 mins at room temperature and followed Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). The immunostaining was performed on a Leica Biosystems BOND^© RX instrument. Counterstained with hematoxylin. Positive staining on the Rat cerebrum. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52919).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ihh antibody [EP1192Y] - BSA and Azide free (AB239853)

The paraffin-embedded sections were Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins and labeling ab52919 at 1/1000 dilutions for 30 mins at room temperature and followed Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). The immunostaining was performed on a Leica Biosystems BOND^© RX instrument. Counterstained with hematoxylin. Positive staining on the Mouse cerebrum. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52919).

• WB

Supplier Data

Western blot - Anti-Ihh antibody [EP1192Y] - BSA and Azide free (AB239853)

Blocking and diluting buffer and concentration : 5% NFDM/TBST. Primary and secondary antibody : incubated for 1h at room temperature. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52919).

All lanes:

Western blot - Anti-Ihh antibody [EP1192Y] (<a href='/en-us/products/primary-antibodies/ihh-antibody-ep1192y-ab52919'>ab52919</a>) at 1/10000 dilution

All lanes:

Human cerebellum lysate at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 45 kDa

false

Exposure time: 10s

• WB

Supplier Data

Western blot - Anti-Ihh antibody [EP1192Y] - BSA and Azide free (AB239853)

Blocking and diluting buffer and concentration : 5% NFDM/TBST. Exposure time : Lane 1, Lane 3 : 10 seconds, Lane 2 : 80 seconds. Primary and secondary antibody : incubated for 1h at room temperature. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52919).

All lanes:

Western blot - Anti-Ihh antibody [EP1192Y] (<a href='/en-us/products/primary-antibodies/ihh-antibody-ep1192y-ab52919'>ab52919</a>) at 1/5000 dilution

Lane 1:

Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate at 15 µg

Lane 2:

NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate at 15 µg

Lane 3:

C6 (Rat glial tumor glial cell) whole cell lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 45 kDa

false

• WB

Supplier Data

Western blot - Anti-Ihh antibody [EP1192Y] - BSA and Azide free (AB239853)

Blocking and diluting buffer and concentration : 5% NFDM/TBST. Primary and secondary antibody : incubated for 1h at room temperature. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52919).

All lanes:

Western blot - Anti-Ihh antibody [EP1192Y] (<a href='/en-us/products/primary-antibodies/ihh-antibody-ep1192y-ab52919'>ab52919</a>) at 1/10000 dilution

All lanes:

His-tagged human Indian Hedgehog (IHH) recombinant protein (aa1-411) at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 45 kDa

false

Exposure time: 180s