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AB62498

Anti-IKAP/p150 antibody

4

(1 Review)

|

(3 Publications)

Rabbit Polyclonal IKAP/p150 antibody. Suitable for ICC, WB, ICC/IF and reacts with Mouse samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human ELP1.

View Alternative Names

IKAP, IKBKAP, ELP1, Elongator complex protein 1, IkappaB kinase complex-associated protein, p150, IKK complex-associated protein

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-IKAP/p150 antibody (AB62498)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-IKAP/p150 antibody (AB62498)

Immunofluorescence of IKAP/p150 in A20 cells using ab62498 at 20 ug/ml.

Western blot - Anti-IKAP/p150 antibody (AB62498)
  • WB

Unknown

Western blot - Anti-IKAP/p150 antibody (AB62498)

Lane 1:

Western blot - Anti-IKAP/p150 antibody (ab62498) at 0.5 µg/mL

Lane 2:

Western blot - Anti-IKAP/p150 antibody (ab62498) at 1 µg/mL

All lanes:

A20 cell lysate at 15 µg

Secondary

All lanes:

anti-rabbit IgG

Predicted band size: 150 kDa

Observed band size: 100 kDa,148 kDa

false

Immunocytochemistry - Anti-IKAP/p150 antibody (AB62498)
  • ICC

Unknown

Immunocytochemistry - Anti-IKAP/p150 antibody (AB62498)

Immunocytochemistry of IKAP/p150 in A20 cells with ab62498 antibody at 1 μg/ml.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

ICC/IF, ICC, WB

applications

Immunogen

Synthetic Peptide within Human ELP1. The exact immunogen used to generate this antibody is proprietary information.

O95163

Specificity

This antibody reacts with IKAP/p150

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1-20 µg/mL", "ICC-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-20 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the elongator complex which is required for multiple tRNA modifications, including mcm5U (5-methoxycarbonylmethyl uridine), mcm5s2U (5-methoxycarbonylmethyl-2-thiouridine), and ncm5U (5-carbamoylmethyl uridine) (PubMed : 29332244). The elongator complex catalyzes the formation of carboxymethyluridine in the wobble base at position 34 in tRNAs (PubMed : 29332244). Regulates the migration and branching of projection neurons in the developing cerebral cortex, through a process depending on alpha-tubulin acetylation (By similarity). ELP1 binds to tRNA, mediating interaction of the elongator complex with tRNA (By similarity). May act as a scaffold protein that assembles active IKK-MAP3K14 complexes (IKKA, IKKB and MAP3K14/NIK) (PubMed : 9751059).
See full target information ELP1
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:67 PubMed39747033

2025

E4F1 coordinates pyruvate metabolism and the activity of the elongator complex to ensure translation fidelity during brain development.

Applications

Unspecified application

Species

Unspecified reactive species

Michela Di Michele,Aurore Attina,Pierre-François Roux,Imène Tabet,Sophie Laguesse,Javier Florido,Morane Houdeville,Armelle Choquet,Betty Encislai,Giuseppe Arena,Carlo De Blasio,Olivia Wendling,François-Xavier Frenois,Laura Papon,Lucille Stuani,Maryse Fuentes,Céline Jahannault Talignani,Mélanie Rousseau,Justine Guégan,Yoan Buscail,Pierrick Dupré,Henri-Alexandre Michaud,Geneviève Rodier,Floriant Bellvert,Hanna Kulyk,Carole Ferraro Peyret,Hugo Mathieu,Pierre Close,Francesca Rapino,Cédric Chaveroux,Nelly Pirot,Lucie Rubio,Adeline Torro,Tania Sorg,Fabrice Ango,Christophe Hirtz,Vincent Compan,Elise Lebigot,Andrea Legati,Daniele Ghezzi,Laurent Nguyen,Alexandre David,Claude Sardet,Matthieu Lacroix,Laurent Le Cam

Journal of biomedical science 28:81 PubMed34819065

2021

Elp1 facilitates RAD51-mediated homologous recombination repair via translational regulation.

Applications

Unspecified application

Species

Unspecified reactive species

Wei-Ting Chen,Huan-Yi Tseng,Chung-Lin Jiang,Chih-Ying Lee,Peter Chi,Liuh-Yow Chen,Kai-Yin Lo,I-Ching Wang,Fu-Jung Lin

Gut 62:452-60 PubMed22637703

2012

Transcriptome analysis identifies TNF superfamily receptors as potential therapeutic targets in alcoholic hepatitis.

Applications

Unspecified application

Species

Unspecified reactive species

Silvia Affò,Marlene Dominguez,Juan José Lozano,Pau Sancho-Bru,Daniel Rodrigo-Torres,Oriol Morales-Ibanez,Montserrat Moreno,Cristina Millán,Aurora Loaeza-del-Castillo,José Altamirano,Juan Carlos García-Pagán,Vicente Arroyo,Pere Ginès,Juan Caballería,Robert F Schwabe,Ramon Bataller
View all publications
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