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AB179437

Anti-IKAP/p150 antibody [EPR13113]

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(3 Publications)

Rabbit Recombinant Monoclonal IKAP/p150 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 3 publications.

View Alternative Names

IKAP, IKBKAP, ELP1, Elongator complex protein 1, IkappaB kinase complex-associated protein, p150, IKK complex-associated protein

3 Images
Flow Cytometry (Intracellular) - Anti-IKAP/p150 antibody [EPR13113] (AB179437)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-IKAP/p150 antibody [EPR13113] (AB179437)

Intracellular flow cytometric analysis of permeabilized Jurkat cellslabeling IKAP/p150with ab179437 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).

Immunocytochemistry/ Immunofluorescence - Anti-IKAP/p150 antibody [EPR13113] (AB179437)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-IKAP/p150 antibody [EPR13113] (AB179437)

Immunofluorescent analysis of HeLa cells labeling IKAP/p150 with ab179437 at 1/50 dilution (green). DAPI nuclear staining (blue).

Western blot - Anti-IKAP/p150 antibody [EPR13113] (AB179437)
  • WB

Supplier Data

Western blot - Anti-IKAP/p150 antibody [EPR13113] (AB179437)

All lanes:

Western blot - Anti-IKAP/p150 antibody [EPR13113] (ab179437) at 1/10000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

Jurkat cell lysate at 10 µg

Lane 4:

293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

Predicted band size: 150 kDa

false

  • Carrier free

    Anti-IKAP/p150 antibody [EPR13113] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13113

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/50", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" } } }

Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Component of the elongator complex which is required for multiple tRNA modifications, including mcm5U (5-methoxycarbonylmethyl uridine), mcm5s2U (5-methoxycarbonylmethyl-2-thiouridine), and ncm5U (5-carbamoylmethyl uridine) (PubMed : 29332244). The elongator complex catalyzes the formation of carboxymethyluridine in the wobble base at position 34 in tRNAs (PubMed : 29332244). Regulates the migration and branching of projection neurons in the developing cerebral cortex, through a process depending on alpha-tubulin acetylation (By similarity). ELP1 binds to tRNA, mediating interaction of the elongator complex with tRNA (By similarity). May act as a scaffold protein that assembles active IKK-MAP3K14 complexes (IKKA, IKKB and MAP3K14/NIK) (PubMed : 9751059).
See full target information ELP1

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

American journal of human genetics 110:531-547 PubMed36809767

2023

Development of an oral treatment that rescues gait ataxia and retinal degeneration in a phenotypic mouse model of familial dysautonomia.

Applications

Unspecified application

Species

Unspecified reactive species

Elisabetta Morini,Anil Chekuri,Emily M Logan,Jessica M Bolduc,Emily G Kirchner,Monica Salani,Aram J Krauson,Jana Narasimhan,Vijayalakshmi Gabbeta,Shivani Grover,Amal Dakka,Anna Mollin,Stephen P Jung,Xin Zhao,Nanjing Zhang,Sophie Zhang,Michael Arnold,Matthew G Woll,Nikolai A Naryshkin,Marla Weetall,Susan A Slaugenhaupt

Nature communications 10:5492 PubMed31792210

2019

Genome-wide CRISPR screen identifies ELP5 as a determinant of gemcitabine sensitivity in gallbladder cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Sunwang Xu,Ming Zhan,Cen Jiang,Min He,Linhua Yang,Hui Shen,Shuai Huang,Xince Huang,Ruirong Lin,Yongheng Shi,Qiang Liu,Wei Chen,Man Mohan,Jian Wang

American journal of human genetics 104:638-650 PubMed30905397

2019

ELP1 Splicing Correction Reverses Proprioceptive Sensory Loss in Familial Dysautonomia.

Applications

Unspecified application

Species

Unspecified reactive species

Elisabetta Morini,Dadi Gao,Connor M Montgomery,Monica Salani,Chiara Mazzasette,Tobias A Krussig,Brooke Swain,Paula Dietrich,Jana Narasimhan,Vijayalakshmi Gabbeta,Amal Dakka,Jean Hedrick,Xin Zhao,Marla Weetall,Nikolai A Naryshkin,Gregory G Wojtkiewicz,Chien-Ping Ko,Michael E Talkowski,Ioannis Dragatsis,Susan A Slaugenhaupt
View all publications

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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