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Rabbit Recombinant Monoclonal Ikaros antibody. Carrier free. Suitable for WB, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ikaros antibody [EPR13790] - BSA and Azide free (AB206645), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ikaros antibody [EPR13790] - BSA and Azide free (AB206645), expandable thumbnail
  • Western blot - Anti-Ikaros antibody [EPR13790] - BSA and Azide free (AB206645), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Ikaros antibody [EPR13790] - BSA and Azide free (AB206645), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBFlow Cyt (Intra)IHC-P
Human
Tested
Tested
Tested
Mouse
Expected
Expected
Tested
Rat
Predicted
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Mouse

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Predicted
Predicted

Species

Rat

Dilution info

-

Notes

-

Associated Products

Select an associated product type

7 products for Alternative Product

8 products for Alternative Version

Target data

Function

Transcription regulator of hematopoietic cell differentiation (PubMed:17934067). Binds gamma-satellite DNA (PubMed:17135265, PubMed:19141594). Plays a role in the development of lymphocytes, B- and T-cells. Binds and activates the enhancer (delta-A element) of the CD3-delta gene. Repressor of the TDT (fikzfterminal deoxynucleotidyltransferase) gene during thymocyte differentiation. Regulates transcription through association with both HDAC-dependent and HDAC-independent complexes. Targets the 2 chromatin-remodeling complexes, NuRD and BAF (SWI/SNF), in a single complex (PYR complex), to the beta-globin locus in adult erythrocytes. Increases normal apoptosis in adult erythroid cells. Confers early temporal competence to retinal progenitor cells (RPCs) (By similarity). Function is isoform-specific and is modulated by dominant-negative inactive isoforms (PubMed:17135265, PubMed:17934067).

Alternative names

IK1, IKAROS, LYF1, ZNFN1A1, IKZF1, ZNFN1A1, LYF1, IK1, IKAROS, DNA-binding protein Ikaros, Ikaros family zinc finger protein 1, Lymphoid transcription factor LyF-1

Recommended products

Rabbit Recombinant Monoclonal Ikaros antibody. Carrier free. Suitable for WB, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR13790

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab206645 is the carrier-free version of Anti-Ikaros antibody [EPR13790] ab191394.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Ikaros also known as IKZF1 is a transcription factor. You also find it being referred to as the Ikaros protein or Ikaros code. It has a molecular mass of about 58-60 kDa. Ikaros expresses mostly in hematopoietic cells including precursors from which various blood cell types derive. It plays a significant role in regulating gene expression and cellular differentiation modulating the chromatin structure to control access to DNA.

Biological function summary

Ikaros protein functions in the regulation of lymphoid cell development. It is a part of a chromatin-remodeling complex influencing chromatin accessibility. Through its zinc finger domains it binds to specific DNA sequences controlling genes necessary for lymphocyte differentiation and proliferation. Ikaros influences various cell types within the immune system especially affecting T and B cell development and also regulates cytokine receptor genes necessary for proper immune function.

Pathways

The Ikaros protein is critical to lymphocyte signaling pathways particularly the JAK/STAT and NF-κB pathways. In these pathways Ikaros interacts with other transcription factors like GATA3 and STAT5 integrating signals that are important for immune cell function and development. This integration allows a coordinated response to extracellular signals ensuring effective immune responses or tolerance.

Associated diseases and disorders

Ikaros is associated with leukemia and lymphomas. Alterations or mutations in ikaros gene can lead to misregulation of its target genes contributing to the development of these diseases by disturbing normal hematopoietic processes. Moreover its interaction with TAL1 protein is significant in certain leukemias where disturbances in the balance of their activities can lead to malignancies. Understanding Ikaros’s role provides insights for therapeutic approaches in these blood disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ikaros antibody [EPR13790] - BSA and Azide free (ab206645), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ikaros antibody [EPR13790] - BSA and Azide free (ab206645)

    This IHC data was generated using the same anti-Ikaros antibody clone, EPR13790, in a different buffer formulation (cat# Anti-Ikaros antibody [EPR13790] ab191394).

    Immunohistochemical analysis of paraffin-embedded Human thymus tissue labeling Ikaros with Anti-Ikaros antibody [EPR13790] ab191394 at 1/1400 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.
    Inset: Negative control: using PBS instead of primary antibody.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ikaros antibody [EPR13790] - BSA and Azide free (ab206645), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ikaros antibody [EPR13790] - BSA and Azide free (ab206645)

    This IHC data was generated using the same anti-Ikaros antibody clone, EPR13790, in a different buffer formulation (cat# Anti-Ikaros antibody [EPR13790] ab191394).

    Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling Ikaros with Anti-Ikaros antibody [EPR13790] ab191394 at 1/1400 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.
    Inset: Negative control: using PBS instead of primary antibody.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-Ikaros antibody [EPR13790] - BSA and Azide free (ab206645), expandable thumbnail

    Western blot - Anti-Ikaros antibody [EPR13790] - BSA and Azide free (ab206645)

    This data was developed using the same antibody clone in a different buffer formulation (Anti-Ikaros antibody [EPR13790] ab191394).

    Lanes 1 - 4: Merged signal (red and green). Green - Anti-Ikaros antibody [EPR13790] ab191394 observed at 50-70 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

    Anti-Ikaros antibody [EPR13790] ab191394 was shown to react with Ikaros in wild-type Jurkat cells in western blot with loss of signal observed in IKZF1 knockout sample. Wild-type and IKZF1 knockout Jurkat cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with Anti-Ikaros antibody [EPR13790] ab191394 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-Ikaros antibody [EPR13790] (Anti-Ikaros antibody [EPR13790] ab191394) at 1/10000 dilution

    Lane 1: Wild-type Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg

    Lane 2: IKZF1 knockout Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg

    Lane 3: Daudi (Human Burkitt's lymphoma cell line) whole cell lysate at 20 µg

    Lane 4: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 57 kDa

    Observed band size: 50-70 kDa

  • Flow Cytometry (Intracellular) - Anti-Ikaros antibody [EPR13790] - BSA and Azide free (ab206645), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Ikaros antibody [EPR13790] - BSA and Azide free (ab206645)

    Intracellular flow cytometric analysis of MOLT4 cells (paraformaldehyde-fixed, 2%) labeling Ikaros with Anti-Ikaros antibody [EPR13790] ab191394 at 1/130 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Ikaros antibody [EPR13790] ab191394).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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