Rabbit Recombinant Monoclonal Ikaros antibody. Carrier free. Suitable for IP, ICC/IF, WB, Flow Cyt (Intra) and reacts with Human samples.
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: PBS
Liquid
Monoclonal
ChIP | IP | ICC/IF | WB | Flow Cyt (Intra) | |
---|---|---|---|---|---|
Human | Not recommended | Tested | Tested | Tested | Tested |
Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info - | Notes - |
Select an associated product type
Transcription regulator of hematopoietic cell differentiation (PubMed:17934067). Binds gamma-satellite DNA (PubMed:17135265, PubMed:19141594). Plays a role in the development of lymphocytes, B- and T-cells. Binds and activates the enhancer (delta-A element) of the CD3-delta gene. Repressor of the TDT (fikzfterminal deoxynucleotidyltransferase) gene during thymocyte differentiation. Regulates transcription through association with both HDAC-dependent and HDAC-independent complexes. Targets the 2 chromatin-remodeling complexes, NuRD and BAF (SWI/SNF), in a single complex (PYR complex), to the beta-globin locus in adult erythrocytes. Increases normal apoptosis in adult erythroid cells. Confers early temporal competence to retinal progenitor cells (RPCs) (By similarity). Function is isoform-specific and is modulated by dominant-negative inactive isoforms (PubMed:17135265, PubMed:17934067).
IK1, IKAROS, LYF1, ZNFN1A1, IKZF1, DNA-binding protein Ikaros, Ikaros family zinc finger protein 1, Lymphoid transcription factor LyF-1
Rabbit Recombinant Monoclonal Ikaros antibody. Carrier free. Suitable for IP, ICC/IF, WB, Flow Cyt (Intra) and reacts with Human samples.
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: PBS
Liquid
Monoclonal
Yes
EPR13791
Affinity purification Protein A
Based on the sequence analysis, this antibody recognizes seven isoforms with the predicted MWs of 58KDa, 48KDa, 48KDa, 43KDa, 41KDa, 32KDa and 53KDa, respectively.
This product is not suitable for Mouse in WB, ICC/IF or IP.
Blue Ice
+4°C
+4°C
Do Not Freeze
ab206644 is the carrier-free version of Anti-Ikaros antibody [EPR13791] - C-terminal ab190691.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This supplementary information is collated from multiple sources and compiled automatically.
Ikaros also known as IKZF1 is a transcription factor. You also find it being referred to as the Ikaros protein or Ikaros code. It has a molecular mass of about 58-60 kDa. Ikaros expresses mostly in hematopoietic cells including precursors from which various blood cell types derive. It plays a significant role in regulating gene expression and cellular differentiation modulating the chromatin structure to control access to DNA.
Ikaros protein functions in the regulation of lymphoid cell development. It is a part of a chromatin-remodeling complex influencing chromatin accessibility. Through its zinc finger domains it binds to specific DNA sequences controlling genes necessary for lymphocyte differentiation and proliferation. Ikaros influences various cell types within the immune system especially affecting T and B cell development and also regulates cytokine receptor genes necessary for proper immune function.
The Ikaros protein is critical to lymphocyte signaling pathways particularly the JAK/STAT and NF-κB pathways. In these pathways Ikaros interacts with other transcription factors like GATA3 and STAT5 integrating signals that are important for immune cell function and development. This integration allows a coordinated response to extracellular signals ensuring effective immune responses or tolerance.
Ikaros is associated with leukemia and lymphomas. Alterations or mutations in ikaros gene can lead to misregulation of its target genes contributing to the development of these diseases by disturbing normal hematopoietic processes. Moreover its interaction with TAL1 protein is significant in certain leukemias where disturbances in the balance of their activities can lead to malignancies. Understanding Ikaros’s role provides insights for therapeutic approaches in these blood disorders.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
This data was developed using the same antibody clone in a different buffer formulation (Anti-Ikaros antibody [EPR13791] - C-terminal ab190691).
Lanes 1 - 4: Merged signal (red and green). Green - Anti-Ikaros antibody [EPR13791] - C-terminal ab190691 observed at 50-70 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
Anti-Ikaros antibody [EPR13791] - C-terminal ab190691 was shown to react with Ikaros in wild-type Jurkat cells in western blot with loss of signal observed in IKZF1 knockout sample. Wild-type and IKZF1 knockout Jurkat cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with Anti-Ikaros antibody [EPR13791] - C-terminal ab190691 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-Ikaros antibody [EPR13791] - C-terminal (Anti-Ikaros antibody [EPR13791] - C-terminal ab190691) at 1/1000 dilution
Lane 1: Wild-type Jurkat cell lysate at 20 µg
Lane 2: IKZF1 knockout Jurkat cell lysate at 20 µg
Lane 3: Daudi cell lysate at 20 µg
Lane 4: HeLa cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 57 kDa
Observed band size: 50-70 kDa
This data was developed using Anti-Ikaros antibody [EPR13791] - C-terminal ab190691, the same antibody clone in a different buffer formulation.
Intracellular Flow Cytometry analysis of Jurkat (human acute T cell leukemia) labelling Ikaros with purified Anti-Ikaros antibody [EPR13791] - C-terminal ab190691 at 1/60 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Alexa Fluor® 488 goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.
This data was developed using Anti-Ikaros antibody [EPR13791] - C-terminal ab190691, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-Ikaros antibody [EPR13791] - C-terminal (Anti-Ikaros antibody [EPR13791] - C-terminal ab190691) at 1/10000 dilution
Lane 1: Raji cell line lysate at 20 µg
Lane 2: Jurkat cell line lysate at 20 µg
Lane 3: Ramos cell line lysate at 20 µg
All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution
Predicted band size: 57 kDa
Observed band size: 50-70 kDa
This data was developed using Anti-Ikaros antibody [EPR13791] - C-terminal ab190691, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde fixed Jurkat cells labeling Ikaros with Anti-Ikaros antibody [EPR13791] - C-terminal ab190691 at 1/250 followed by Goat anti rabbit IgG (Alexa Fluor® 555) at 1/200 and conterstained with DAPI.
50-70 kDa Ikaros was immunoprecipitated from 0.35mg Ramos (human Burkitt's lymphoma B lymphocyte) whole cell lysate with Anti-Ikaros antibody [EPR13791] - C-terminal ab190691 at 1/30 dilution. Western blot was performed from the immunoprecipitate using Anti-Ikaros antibody [EPR13791] - C-terminal ab190691 at 1/1000 dilution (0.71 μg/ml). VeriBlot for IP secondary antibody (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1 (Input): Ramos (human Burkitt's lymphoma B lymphocyte) whole cell lysate 10 μg.
Lane 2 (+): Ramos whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Ikaros antibody [EPR13791] - C-terminal ab190691 in Ramos whole cell lysate.
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 15 seconds.
This data was developed using the same antibody clone in a different buffer formulation (Anti-Ikaros antibody [EPR13791] - C-terminal ab190691).
All lanes: Immunoprecipitation - Anti-Ikaros antibody [EPR13791] - C-terminal (Anti-Ikaros antibody [EPR13791] - C-terminal ab190691) at 1/30 dilution
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Predicted band size: 57 kDa
Observed band size: 50-70 kDa
Exposure time: 15s
50-70 kDa Ikaros was immunoprecipitated from 0.35mg Ramos (human Burkitt's lymphoma B lymphocyte) whole cell lysate with Anti-Ikaros antibody [EPR13791] - C-terminal ab190691 at 1/30 dilution. Western blot was performed from the immunoprecipitate using Anti-Ikaros antibody [EPR13791] - C-terminal ab190691 at 1/1000 dilution (0.71 μg/ml). VeriBlot for IP secondary antibody (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1 (Input): Ramos (human Burkitt's lymphoma B lymphocyte) whole cell lysate 10 μg.
Lane 2 (+): Ramos whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Ikaros antibody [EPR13791] - C-terminal ab190691 in Ramos whole cell lysate.
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 15 seconds.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com