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Rabbit Recombinant Monoclonal Ikaros antibody. C-terminal. Suitable for WB, ICC/IF, Flow Cyt (Intra), IP and reacts with Human samples.

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Images

Western blot - Anti-Ikaros antibody [EPR13791] - C-terminal (AB190691), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Ikaros antibody [EPR13791] - C-terminal (AB190691), expandable thumbnail
  • Western blot - Anti-Ikaros antibody [EPR13791] - C-terminal (AB190691), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Ikaros antibody [EPR13791] - C-terminal (AB190691), expandable thumbnail
  • Immunoprecipitation - Anti-Ikaros antibody [EPR13791] - C-terminal (AB190691), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFFlow Cyt (Intra)IPChIP
Human
Tested
Tested
Tested
Tested
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Human

Dilution info

1/1000 - 1/10000

Notes

-

Not recommended
Not recommended

Species

Mouse

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/250

Notes

-

Not recommended
Not recommended

Species

Mouse

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/60

Notes

-

Not recommended
Not recommended

Species

Mouse

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/30

Notes

-

Not recommended
Not recommended

Species

Mouse

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse

Dilution info

-

Notes

-

Associated Products

Select an associated product type

8 products for Alternative Product

Target data

Function

Transcription regulator of hematopoietic cell differentiation (PubMed:17934067). Binds gamma-satellite DNA (PubMed:17135265, PubMed:19141594). Plays a role in the development of lymphocytes, B- and T-cells. Binds and activates the enhancer (delta-A element) of the CD3-delta gene. Repressor of the TDT (fikzfterminal deoxynucleotidyltransferase) gene during thymocyte differentiation. Regulates transcription through association with both HDAC-dependent and HDAC-independent complexes. Targets the 2 chromatin-remodeling complexes, NuRD and BAF (SWI/SNF), in a single complex (PYR complex), to the beta-globin locus in adult erythrocytes. Increases normal apoptosis in adult erythroid cells. Confers early temporal competence to retinal progenitor cells (RPCs) (By similarity). Function is isoform-specific and is modulated by dominant-negative inactive isoforms (PubMed:17135265, PubMed:17934067).

Alternative names

Recommended products

Rabbit Recombinant Monoclonal Ikaros antibody. C-terminal. Suitable for WB, ICC/IF, Flow Cyt (Intra), IP and reacts with Human samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR13791

Purification technique

Affinity purification Protein A

Specificity

Based on the sequence analysis, ab190691 recognizes seven isoforms with the predicted MWs of 58KDa, 48KDa, 48KDa, 43KDa, 41KDa, 32KDa and 53KDa, respectively.

This product is not suitable for Mouse in WB, ICC/IF or IP.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-Ikaros antibody [EPR13791] - C-terminal (ab190691), expandable thumbnail

    Western blot - Anti-Ikaros antibody [EPR13791] - C-terminal (ab190691)

    Lanes 1 - 4: Merged signal (red and green). Green - ab190691 observed at 50-70 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

    ab190691 was shown to react with Ikaros in wild-type Jurkat cells in western blot with loss of signal observed in IKZF1 knockout sample. Wild-type and IKZF1 knockout Jurkat cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab190691 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-Ikaros antibody [EPR13791] - C-terminal (ab190691) at 1/1000 dilution

    Lane 1: Wild-type Jurkat cell lysate at 20 µg

    Lane 2: IKZF1 knockout Jurkat cell lysate at 20 µg

    Lane 3: Daudi cell lysate at 20 µg

    Lane 4: HeLa cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 57 kDa

    Observed band size: 50-70 kDa

  • Flow Cytometry (Intracellular) - Anti-Ikaros antibody [EPR13791] - C-terminal (ab190691), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Ikaros antibody [EPR13791] - C-terminal (ab190691)

    Intracellular Flow Cytometry analysis of Jurkat (human acute T cell leukemia) labelling Ikaros with purified ab190691 at 1/60 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Alexa Fluor® 488 goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

  • Western blot - Anti-Ikaros antibody [EPR13791] - C-terminal (ab190691), expandable thumbnail

    Western blot - Anti-Ikaros antibody [EPR13791] - C-terminal (ab190691)

    All lanes: Western blot - Anti-Ikaros antibody [EPR13791] - C-terminal (ab190691) at 1/10000 dilution

    Lane 1: Raji cell line lysate at 20 µg

    Lane 2: Jurkat cell line lysate at 20 µg

    Lane 3: Ramos cell line lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

    Predicted band size: 57 kDa

    Observed band size: 50-70 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Ikaros antibody [EPR13791] - C-terminal (ab190691), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Ikaros antibody [EPR13791] - C-terminal (ab190691)

    Immunofluorescent analysis of 4% paraformaldehyde fixed Jurkat cells labeling Ikaros with ab190691 at 1/250 followed by Goat anti rabbit IgG (Alexa Fluor® 555) at 1/200 and conterstained with DAPI.

  • Immunoprecipitation - Anti-Ikaros antibody [EPR13791] - C-terminal (ab190691), expandable thumbnail

    Immunoprecipitation - Anti-Ikaros antibody [EPR13791] - C-terminal (ab190691)

    50-70 kDa Ikaros was immunoprecipitated from 0.35mg Ramos (human Burkitt's lymphoma B lymphocyte) whole cell lysate with ab190691 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab190691 at 1/1000 dilution (0.71 μg/ml). VeriBlot for IP secondary antibody (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1 (Input): Ramos (human Burkitt's lymphoma B lymphocyte) whole cell lysate 10 μg.

    Lane 2 (+): Ramos whole cell lysate.

    Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab190691 in Ramos whole cell lysate.

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: 15 seconds.

    All lanes: Immunoprecipitation - Anti-Ikaros antibody [EPR13791] - C-terminal (ab190691) at 1/30 dilution

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Predicted band size: 57 kDa

    Observed band size: 50-70 kDa

    Exposure time: 15s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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