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Rabbit Monoclonal Ikaros antibody. Suitable for WB, IHC-P, IHC-Fr, Flow Cyt (Intra), IP, ChIP-seq and reacts with Human, Mouse, Rat samples.

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Images

ChIP-sequencing - Anti-Ikaros antibody [EPR25259-176] (AB300405), expandable thumbnail
  • ChIP-sequencing - Anti-Ikaros antibody [EPR25259-176] (AB300405), expandable thumbnail
  • ChIP-sequencing - Anti-Ikaros antibody [EPR25259-176] (AB300405), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-Ikaros antibody [EPR25259-176] (AB300405), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-Ikaros antibody [EPR25259-176] (AB300405), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PIHC-FrICC/IFFlow Cyt (Intra)IPChIPChIP-seq
Human
Tested
Tested
Expected
Not recommended
Tested
Tested
Not recommended
Tested
Mouse
Tested
Tested
Tested
Not recommended
Tested
Tested
Not recommended
Expected
Rat
Tested
Tested
Tested
Not recommended
Expected
Expected
Not recommended
Expected

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Species

Mouse

Dilution info

1/1000

Notes

-

Species

Rat

Dilution info

1/1000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/2000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Mouse

Dilution info

1/2000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/2000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species

Mouse

Dilution info

1/100

Notes

Not Applicable.

Species

Rat

Dilution info

1/100

Notes

Not Applicable.

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/500

Notes

-

Species

Mouse

Dilution info

1/500

Notes

-

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

1/30

Notes

-

Species

Mouse

Dilution info

1/30

Notes

-

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

8 µg for 107 Cells

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Target data

Function

Transcription regulator of hematopoietic cell differentiation (PubMed:17934067). Binds gamma-satellite DNA (PubMed:17135265, PubMed:19141594). Plays a role in the development of lymphocytes, B- and T-cells. Binds and activates the enhancer (delta-A element) of the CD3-delta gene. Repressor of the TDT (fikzfterminal deoxynucleotidyltransferase) gene during thymocyte differentiation. Regulates transcription through association with both HDAC-dependent and HDAC-independent complexes. Targets the 2 chromatin-remodeling complexes, NuRD and BAF (SWI/SNF), in a single complex (PYR complex), to the beta-globin locus in adult erythrocytes. Increases normal apoptosis in adult erythroid cells. Confers early temporal competence to retinal progenitor cells (RPCs) (By similarity). Function is isoform-specific and is modulated by dominant-negative inactive isoforms (PubMed:17135265, PubMed:17934067).

Alternative names

Recommended products

Rabbit Monoclonal Ikaros antibody. Suitable for WB, IHC-P, IHC-Fr, Flow Cyt (Intra), IP, ChIP-seq and reacts with Human, Mouse, Rat samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR25259-176

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Ikaros also known as IKZF1 is a transcription factor. You also find it being referred to as the Ikaros protein or Ikaros code. It has a molecular mass of about 58-60 kDa. Ikaros expresses mostly in hematopoietic cells including precursors from which various blood cell types derive. It plays a significant role in regulating gene expression and cellular differentiation modulating the chromatin structure to control access to DNA.

Biological function summary

Ikaros protein functions in the regulation of lymphoid cell development. It is a part of a chromatin-remodeling complex influencing chromatin accessibility. Through its zinc finger domains it binds to specific DNA sequences controlling genes necessary for lymphocyte differentiation and proliferation. Ikaros influences various cell types within the immune system especially affecting T and B cell development and also regulates cytokine receptor genes necessary for proper immune function.

Pathways

The Ikaros protein is critical to lymphocyte signaling pathways particularly the JAK/STAT and NF-κB pathways. In these pathways Ikaros interacts with other transcription factors like GATA3 and STAT5 integrating signals that are important for immune cell function and development. This integration allows a coordinated response to extracellular signals ensuring effective immune responses or tolerance.

Associated diseases and disorders

Ikaros is associated with leukemia and lymphomas. Alterations or mutations in ikaros gene can lead to misregulation of its target genes contributing to the development of these diseases by disturbing normal hematopoietic processes. Moreover its interaction with TAL1 protein is significant in certain leukemias where disturbances in the balance of their activities can lead to malignancies. Understanding Ikaros’s role provides insights for therapeutic approaches in these blood disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

18 product images

  • ChIP-sequencing - Anti-Ikaros antibody [EPR25259-176] (ab300405), expandable thumbnail

    ChIP-sequencing - Anti-Ikaros antibody [EPR25259-176] (ab300405)

    Chromatin was prepared from Raji cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 107 cells and 8 µg of ab300405 [EPR25259-176]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads. The input control is also shown.

  • ChIP-sequencing - Anti-Ikaros antibody [EPR25259-176] (ab300405), expandable thumbnail

    ChIP-sequencing - Anti-Ikaros antibody [EPR25259-176] (ab300405)

    Chromatin was prepared from Raji cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 107 cells and 8 µg of ab300405 [EPR25259-176]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads. The input control is also shown.

  • ChIP-sequencing - Anti-Ikaros antibody [EPR25259-176] (ab300405), expandable thumbnail

    ChIP-sequencing - Anti-Ikaros antibody [EPR25259-176] (ab300405)

    Chromatin was prepared from Raji cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 107 cells and 8 µg of ab300405 [EPR25259-176]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads. The input control is also shown.

  • Immunohistochemistry (Frozen sections) - Anti-Ikaros antibody [EPR25259-176] (ab300405), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Ikaros antibody [EPR25259-176] (ab300405)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat kidney (fresh) tissue labeling Ikaros with ab300405 at 1/100 (5.39 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Negative control (PMID: 1439790)No staining on rat kidney is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution. .

  • Immunohistochemistry (Frozen sections) - Anti-Ikaros antibody [EPR25259-176] (ab300405), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Ikaros antibody [EPR25259-176] (ab300405)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat thymus (fresh) tissue labeling Ikaros with ab300405 at 1/100 (5.39 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Positive staining on rat thymus is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution. .

  • Immunohistochemistry (Frozen sections) - Anti-Ikaros antibody [EPR25259-176] (ab300405), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Ikaros antibody [EPR25259-176] (ab300405)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse kidney (fresh) tissue labeling Ikaros with ab300405 at 1/100 (5.39 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Negative control (PMID: 1439790)No staining on mouse kidney is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution. .

  • Immunohistochemistry (Frozen sections) - Anti-Ikaros antibody [EPR25259-176] (ab300405), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Ikaros antibody [EPR25259-176] (ab300405)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse thymus (fresh) tissue labeling Ikaros with ab300405 at 1/100 (5.39 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Positive staining on mouse thymus is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution. .

  • Immunoprecipitation - Anti-Ikaros antibody [EPR25259-176] (ab300405), expandable thumbnail

    Immunoprecipitation - Anti-Ikaros antibody [EPR25259-176] (ab300405)

    Ikaros was immunoprecipitated from 0.35 mg EL4 (mouse lymphoma t lymphocyte) whole cell lysate 10 ug with ab300405 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300405 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: EL4 (mouse lymphoma t lymphocyte) whole cell lysate 10 ug

    Lane 2: abAB300405 IP in EL4 whole cell lysate

    Lane 3:Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab300405 in EL4 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 8 seconds.

    All lanes: Immunoprecipitation - Anti-Ikaros antibody [EPR25259-176] (ab300405) at 1/1000 dilution

    All lanes: EL4 whole cell lysate at 10 µg

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Exposure time: 8s

  • Immunoprecipitation - Anti-Ikaros antibody [EPR25259-176] (ab300405), expandable thumbnail

    Immunoprecipitation - Anti-Ikaros antibody [EPR25259-176] (ab300405)

    Ikaros was immunoprecipitated from 0.35 mg Raji (human burkitt's lymphoma b lymphocyte) whole cell lysate 10 ug with ab300405 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300405 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: Raji (human burkitt's lymphoma b lymphocyte) whole cell lysate 10 ug

    Lane 2: abAB300405 IP in Raji whole cell lysate

    Lane 3:Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab300405 in Raji whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 8 seconds.

    All lanes: Immunoprecipitation - Anti-Ikaros antibody [EPR25259-176] (ab300405) at 1/1000 dilution

    All lanes: Raji whole cell lysate at 10 µg

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Exposure time: 8s

  • Flow Cytometry (Intracellular) - Anti-Ikaros antibody [EPR25259-176] (ab300405), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Ikaros antibody [EPR25259-176] (ab300405)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized EL4 (mouse lymphoma T lymphocyte) cells labelling Ikaros with ab300405 at 1/500 dilution (0.1ug) (Red) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody.

  • Flow Cytometry (Intracellular) - Anti-Ikaros antibody [EPR25259-176] (ab300405), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Ikaros antibody [EPR25259-176] (ab300405)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell, Left) / Jurkat (human T cell leukemia T lymphocyte, Right) cells labelling Ikaros with ab300405 at 1/500 dilution (0.1ug) (Red) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody. Negative control: HeLa(HPA data)

  • Western blot - Anti-Ikaros antibody [EPR25259-176] (ab300405), expandable thumbnail

    Western blot - Anti-Ikaros antibody [EPR25259-176] (ab300405)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    All lanes: Western blot - Anti-Ikaros antibody [EPR25259-176] (ab300405) at 1/1000 dilution

    Lane 1: His-tagged human Ikaros/IKZF1 recombinant protein

    Lane 2: GST-tagged human Helios/IKZF2 recombinant protein

    Lane 3: GST-tagged human Aiolos/IKZF3 recombinant protein

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Performed under reducing conditions.

    Observed band size: 16 kDa

    Exposure time: 3s

  • Western blot - Anti-Ikaros antibody [EPR25259-176] (ab300405), expandable thumbnail

    Western blot - Anti-Ikaros antibody [EPR25259-176] (ab300405)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    All lanes: Western blot - Anti-Ikaros antibody [EPR25259-176] (ab300405) at 1/1000 dilution

    Lane 1: Rat thymus tissue lysate

    Lane 2: Rat spleen tissue lysate

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Performed under reducing conditions.

    Observed band size: 30 kDa, 70 kDa

    Exposure time: 70s

  • Western blot - Anti-Ikaros antibody [EPR25259-176] (ab300405), expandable thumbnail

    Western blot - Anti-Ikaros antibody [EPR25259-176] (ab300405)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    All lanes: Western blot - Anti-Ikaros antibody [EPR25259-176] (ab300405) at 1/1000 dilution

    Lane 1: Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

    Lane 2: Raji (human burkitts lymphoma b lymphocyte) whole cell lysate

    Lane 3: Daudi (human burkitts lymphoma lymphoblast) whole cell lysate

    Lane 4: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate

    Lane 5: EL4 (mouse lymphoma t lymphocyte) whole cell lysate

    Lane 6: Mouse spleen tissue lysate

    Lane 7: Mouse thymus tissue lysate

    Lane 8: Mouse kidney tissue lysate

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Performed under reducing conditions.

    Observed band size: 30 kDa, 70 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ikaros antibody [EPR25259-176] (ab300405), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ikaros antibody [EPR25259-176] (ab300405)

    Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling Ikaros with ab300405 at 1/2000 (0.27 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Nuclear staining on rat spleen.The section was incubated with ab300405 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ikaros antibody [EPR25259-176] (ab300405), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ikaros antibody [EPR25259-176] (ab300405)

    Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling Ikaros with ab300405 at 1/2000 (0.27 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Nuclear staining on mouse spleen.The section was incubated with ab300405 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ikaros antibody [EPR25259-176] (ab300405), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ikaros antibody [EPR25259-176] (ab300405)

    Immunohistochemical analysis of paraffin-embedded Human thymus tissue labeling Ikaros with ab300405 at 1/2000 (0.27 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Nuclear staining on human thymus (PMID: 8964602).The section was incubated with ab300405 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ikaros antibody [EPR25259-176] (ab300405), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ikaros antibody [EPR25259-176] (ab300405)

    Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling Ikaros with ab300405 at 1/2000 (0.27 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Nuclear staining on human spleen (PMID: 8964602).The section was incubated with ab300405 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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