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Rabbit Polyclonal Ikaros antibody. N-terminal. Suitable for IP, ChIP, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human IKZF1_HUMAN.

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Images

ChIP - Anti-Ikaros antibody - N-terminal (AB229275), expandable thumbnail
  • Western blot - Anti-Ikaros antibody - N-terminal (AB229275), expandable thumbnail
  • Immunoprecipitation - Anti-Ikaros antibody - N-terminal (AB229275), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human IKZF1_HUMAN. The exact immunogen used to generate this antibody is proprietary information. Database link Q13422

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPChIPWB
Human
Tested
Tested
Tested
Mouse
Predicted
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

1/100 - 1/500

Notes

-

Predicted
Predicted

Species

Mouse

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Predicted
Predicted

Species

Mouse

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/500 - 1/3000

Notes

-

Predicted
Predicted

Species

Mouse

Dilution info

-

Notes

-

Associated Products

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Target data

Function

Transcription regulator of hematopoietic cell differentiation (PubMed:17934067). Binds gamma-satellite DNA (PubMed:17135265, PubMed:19141594). Plays a role in the development of lymphocytes, B- and T-cells. Binds and activates the enhancer (delta-A element) of the CD3-delta gene. Repressor of the TDT (fikzfterminal deoxynucleotidyltransferase) gene during thymocyte differentiation. Regulates transcription through association with both HDAC-dependent and HDAC-independent complexes. Targets the 2 chromatin-remodeling complexes, NuRD and BAF (SWI/SNF), in a single complex (PYR complex), to the beta-globin locus in adult erythrocytes. Increases normal apoptosis in adult erythroid cells. Confers early temporal competence to retinal progenitor cells (RPCs) (By similarity). Function is isoform-specific and is modulated by dominant-negative inactive isoforms (PubMed:17135265, PubMed:17934067).

Alternative names

Recommended products

Rabbit Polyclonal Ikaros antibody. N-terminal. Suitable for IP, ChIP, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human IKZF1_HUMAN.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human IKZF1_HUMAN. The exact immunogen used to generate this antibody is proprietary information. Database link Q13422
Purification technique

Affinity purification Immunogen

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Ikaros also known as IKZF1 is a transcription factor. You also find it being referred to as the Ikaros protein or Ikaros code. It has a molecular mass of about 58-60 kDa. Ikaros expresses mostly in hematopoietic cells including precursors from which various blood cell types derive. It plays a significant role in regulating gene expression and cellular differentiation modulating the chromatin structure to control access to DNA.

Biological function summary

Ikaros protein functions in the regulation of lymphoid cell development. It is a part of a chromatin-remodeling complex influencing chromatin accessibility. Through its zinc finger domains it binds to specific DNA sequences controlling genes necessary for lymphocyte differentiation and proliferation. Ikaros influences various cell types within the immune system especially affecting T and B cell development and also regulates cytokine receptor genes necessary for proper immune function.

Pathways

The Ikaros protein is critical to lymphocyte signaling pathways particularly the JAK/STAT and NF-κB pathways. In these pathways Ikaros interacts with other transcription factors like GATA3 and STAT5 integrating signals that are important for immune cell function and development. This integration allows a coordinated response to extracellular signals ensuring effective immune responses or tolerance.

Associated diseases and disorders

Ikaros is associated with leukemia and lymphomas. Alterations or mutations in ikaros gene can lead to misregulation of its target genes contributing to the development of these diseases by disturbing normal hematopoietic processes. Moreover its interaction with TAL1 protein is significant in certain leukemias where disturbances in the balance of their activities can lead to malignancies. Understanding Ikaros’s role provides insights for therapeutic approaches in these blood disorders.

Product promise

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3 product images

  • ChIP - Anti-Ikaros antibody - N-terminal (ab229275), expandable thumbnail

    ChIP - Anti-Ikaros antibody - N-terminal (ab229275)

    Cross-linked ChIP was performed with Jurkat (Human T cell leukemia cell line from peripheral blood) chromatin extract and 5 μg of either control rabbit IgG or ab229275. The precipitated DNA was detected by PCR with primer set targeting to VPAC-1 receptor promoter.

  • Western blot - Anti-Ikaros antibody - N-terminal (ab229275), expandable thumbnail

    Western blot - Anti-Ikaros antibody - N-terminal (ab229275)

    7.5% SDS-PAGE

    All lanes: Western blot - Anti-Ikaros antibody - N-terminal (ab229275) at 1/500 dilution

    Lane 1: Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate/extract at 50 µg

    Lane 2: Raji (human Burkitt's lymphoma cell line) whole cell lysate/extract at 50 µg

    Lane 3: NCI-H929 whole cell lysate/extract at 50 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG

    Developed using the ECL technique.

    Predicted band size: 57 kDa

  • Immunoprecipitation - Anti-Ikaros antibody - N-terminal (ab229275), expandable thumbnail

    Immunoprecipitation - Anti-Ikaros antibody - N-terminal (ab229275)

    Ikaros was immunoprecipitated from Raji (human Burkitt's lymphoma cell line) whole cell extract with 4 μg ab229275. Western blot was performed from the immunoprecipitate using ab229275 at 1/500 dilution.

    Lane 1: Control IgG IP in Raji whole cell extract.

    Lane 2: ab229275 IP in Raji whole cell extract.

    7.5% SDS-PAGE

    All lanes: Immunoprecipitation - Anti-Ikaros antibody - N-terminal (ab229275)

    Developed using the ECL technique.

    Predicted band size: 57 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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