AB229275

Anti-Ikaros antibody - N-terminal

Name: Anti-Ikaros antibody - N-terminal (ab229275) | Abcam
Brand: Abcam Limited
SKU: AB229275
Price: 525 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal Ikaros antibody. N-terminal. Suitable for IP, ChIP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human IKZF1.

View Alternative Names

IK1, IKAROS, LYF1, ZNFN1A1, IKZF1, DNA-binding protein Ikaros, Ikaros family zinc finger protein 1, Lymphoid transcription factor LyF-1

3 Images

Supplier Data

Immunoprecipitation - Anti-Ikaros antibody - N-terminal (AB229275)

Ikaros was immunoprecipitated from Raji (human Burkitt's lymphoma cell line) whole cell extract with 4 μg ab229275. Western blot was performed from the immunoprecipitate using ab229275 at 1/500 dilution.

Lane 1 : Control IgG IP in Raji whole cell extract.

Lane 2 : ab229275 IP in Raji whole cell extract.

7.5% SDS-PAGE

All lanes:

Immunoprecipitation - Anti-Ikaros antibody - N-terminal (ab229275)

Predicted band size: 57 kDa

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ChIP - Anti-Ikaros antibody - N-terminal (AB229275)

ChIP

Supplier Data

ChIP - Anti-Ikaros antibody - N-terminal (AB229275)

Cross-linked ChIP was performed with Jurkat (Human T cell leukemia cell line from peripheral blood) chromatin extract and 5 μg of either control rabbit IgG or ab229275. The precipitated DNA was detected by PCR with primer set targeting to VPAC-1 receptor promoter.

Supplier Data

Western blot - Anti-Ikaros antibody - N-terminal (AB229275)

7.5% SDS-PAGE

All lanes:

Western blot - Anti-Ikaros antibody - N-terminal (ab229275) at 1/500 dilution

Lane 1:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate/extract at 50 µg

Lane 2:

Raji (human Burkitt's lymphoma cell line) whole cell lysate/extract at 50 µg

Lane 3:

NCI-H929 whole cell lysate/extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 57 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP, ChIP

applications

Immunogen

Recombinant Fragment Protein within Human IKZF1. The exact immunogen used to generate this antibody is proprietary information.

Q13422

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/500", "IP-species-notes": "", "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ikaros also known as IKZF1 is a transcription factor. You also find it being referred to as the Ikaros protein or Ikaros code. It has a molecular mass of about 58-60 kDa. Ikaros expresses mostly in hematopoietic cells including precursors from which various blood cell types derive. It plays a significant role in regulating gene expression and cellular differentiation modulating the chromatin structure to control access to DNA.

Biological function summary

Ikaros protein functions in the regulation of lymphoid cell development. It is a part of a chromatin-remodeling complex influencing chromatin accessibility. Through its zinc finger domains it binds to specific DNA sequences controlling genes necessary for lymphocyte differentiation and proliferation. Ikaros influences various cell types within the immune system especially affecting T and B cell development and also regulates cytokine receptor genes necessary for proper immune function.

Pathways

The Ikaros protein is critical to lymphocyte signaling pathways particularly the JAK/STAT and NF-κB pathways. In these pathways Ikaros interacts with other transcription factors like GATA3 and STAT5 integrating signals that are important for immune cell function and development. This integration allows a coordinated response to extracellular signals ensuring effective immune responses or tolerance.

Ikaros is associated with leukemia and lymphomas. Alterations or mutations in ikaros gene can lead to misregulation of its target genes contributing to the development of these diseases by disturbing normal hematopoietic processes. Moreover its interaction with TAL1 protein is significant in certain leukemias where disturbances in the balance of their activities can lead to malignancies. Understanding Ikaros’s role provides insights for therapeutic approaches in these blood disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcription regulator of hematopoietic cell differentiation (PubMed : 17934067). Binds gamma-satellite DNA (PubMed : 17135265, PubMed : 19141594). Plays a role in the development of lymphocytes, B- and T-cells. Binds and activates the enhancer (delta-A element) of the CD3-delta gene. Repressor of the TDT (fikzfterminal deoxynucleotidyltransferase) gene during thymocyte differentiation. Regulates transcription through association with both HDAC-dependent and HDAC-independent complexes. Targets the 2 chromatin-remodeling complexes, NuRD and BAF (SWI/SNF), in a single complex (PYR complex), to the beta-globin locus in adult erythrocytes. Increases normal apoptosis in adult erythroid cells. Confers early temporal competence to retinal progenitor cells (RPCs) (By similarity). Function is isoform-specific and is modulated by dominant-negative inactive isoforms (PubMed : 17135265, PubMed : 17934067).

See full target information IKZF1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Aging 13: PubMed33493132

2021

RNF168 is highly expressed in esophageal squamous cell carcinoma and contributes to the malignant behaviors in association with the Wnt/β-catenin signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yunjiu Gou,Dacheng Jin,Shengliang He,Songchen Han,Qizhou Bai

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Ikaros antibody - N-terminal

Immunoprecipitation - Anti-Ikaros antibody - N-terminal (AB229275)

ChIP - Anti-Ikaros antibody - N-terminal (AB229275)

Western blot - Anti-Ikaros antibody - N-terminal (AB229275)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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