Rabbit Polyclonal IKB alpha antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NFKBIA.
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Publications
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- Allergologia et immunopathologia 50:119-1242022PTPRO activates TLR4/NF-κB signaling to intensify lipopolysaccharide-induced pneumonia cell injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYao Chen,Buming SunPubMed 35527665
- International journal of molecular sciences 22:2021Suppression of LPS-Induced Inflammation and Cell Migration by Azelastine through Inhibition of JNK/NF-κB Pathway in BV2 Microglial Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPhuong Linh Nguyen,Bich Phuong Bui,Men Thi Hoai Duong,Kyeong Lee,Hee-Chul Ahn,Jungsook ChoPubMed 34445767
- Journal of gastroenterology 56:442-4552021Lactobacillus stress protein GroEL prevents colonic inflammation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlexandre M M Dias,Romain Douhard,François Hermetet,Mathilde Regimbeau,Tatiana E Lopez,Daniel Gonzalez,Sophie Masson,Guillaume Marcion,Killian Chaumonnot,Burhan Uyanik,Sébastien Z Causse,Aurélie Rieu,Tarik Hadi,Christelle Basset,Johanna Chluba,Jacques Grober,Jean Guzzo,Fabrice Neiers,Pablo Ortega-Deballon,Oleg N Demidov,Frédéric Lirussi,Carmen GarridoPubMed 33782752
- Oncology reports 45:2021MicroRNA‑129 inhibits colorectal cancer cell proliferation, invasion and epithelial‑to‑mesenchymal transition by targeting SOX4.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhiping Chen,Tianyu Zhong,Jinghua Zhong,Yang Tang,Baodian Ling,Lanfeng WangPubMed 33760159
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human NFKBIA. The exact immunogen used to generate this antibody is proprietary information. Database link P25963
Consider this alternative
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Tested | Tested | Expected |
| Rat | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted |
| Pig | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 - 1/10000 | Notes - |
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow, Pig | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 - 1/1000 | Notes - |
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow, Pig | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow, Pig | Dilution info - | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Function
Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL (RELA/p65 and NFKB1/p50) dimers in the cytoplasm by masking their nuclear localization signals (PubMed:1493333, PubMed:36651806, PubMed:7479976). On cellular stimulation by immune and pro-inflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription (PubMed:7479976, PubMed:7628694, PubMed:7796813, PubMed:7878466).
Alternative names
IKBA, MAD3, NFKBI, NFKBIA, NF-kappa-B inhibitor alpha, I-kappa-B-alpha, Major histocompatibility complex enhancer-binding protein MAD3, IkB-alpha, IkappaBalpha
Recommended products
Rabbit Polyclonal IKB alpha antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NFKBIA.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human NFKBIA. The exact immunogen used to generate this antibody is proprietary information. Database link P25963
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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4 product images
Western blot - Anti-IKB alpha antibody (ab97783)
10 % SDS-PAGE
All lanes: Western blot - Anti-IKB alpha antibody (ab97783) at 1/5000 dilution
Lane 1: BCL-1 whole cell lysate at 30 µg
Lane 2: C2C12 whole cell lysate at 30 µg
Predicted band size: 35 kDa
Western blot - Anti-IKB alpha antibody (ab97783)
10% SDS-PAGE.
All lanes: Western blot - Anti-IKB alpha antibody (ab97783) at 1/1000 dilution
All lanes: HepG2 whole cell lysate at 30 µg
Predicted band size: 35 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IKB alpha antibody (ab97783)
ab97783 at 1/500 dilution detects IKB alpha protein at nucleus on U251 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U251 xenograft.
Immunocytochemistry/ Immunofluorescence - Anti-IKB alpha antibody (ab97783)
ab97783 at 1/200 dilution staining IKB alpha in HeLa cells by Immunofluorescence, Paraformaldehyde fixed. Lower image is merged with DNA probe.
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