Anti-IKB alpha antibody

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-IKB alpha antibody (AB97783)

ab97783 at 1/200 dilution staining IKB alpha in HeLa cells by Immunofluorescence, Paraformaldehyde fixed. Lower image is merged with DNA probe.

• WB

Lab

Western blot - Anti-IKB alpha antibody (AB97783)

Western blot : Rabbit Polyclonal to IKB alpha ab97783 staining at 1/1000 dilution, shown in green; Mouse anti alpha Tubulin (ab7291) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 38 kDa in Wild-type Jurkat cell lysates with no signal observed at this size in NFKBIA knockout Jurkat cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-IKB alpha antibody (ab97783) at 1/1000 dilution

Lane 1:

Wild-type Jurkat at 20 µg

Lane 2:

Western blot - Human NFKBIA knockout Jurkat cell line (<a href='/en-us/products/cell-lines/human-nfkbia-knockout-jurkat-cell-line-ab302178'>ab302178</a>) at 20 µg

Lane 3:

HeLa at 20 µg

Secondary

Lanes 1 - 3:

Goat anti-Rabbit 800CW at 1/20000 dilution

Lanes 1 - 3:

Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 36 kDa

Observed band size: 38 kDa,50 kDa

false

• WB

Supplier Data

Western blot - Anti-IKB alpha antibody (AB97783)

Samples were separated by 12% SDS-PAGE.

All lanes:

Western blot - Anti-IKB alpha antibody (ab97783) at 1/1000 dilution

Lane 1:

Non-transfected (-) HT-29 whole cell extracts at 30 µg

Lane 2:

Transfected (+) HT-29 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 36 kDa

false

• WB

Supplier Data

Western blot - Anti-IKB alpha antibody (AB97783)

10 % SDS-PAGE

All lanes:

Western blot - Anti-IKB alpha antibody (ab97783) at 1/5000 dilution

Lane 1:

BCL-1 whole cell lysate at 30 µg

Lane 2:

C2C12 whole cell lysate at 30 µg

Predicted band size: 35 kDa

false