Rabbit Recombinant Monoclonal IKB alpha phospho S32 antibody. Suitable for IP, Dot, WB and reacts with Human, Mouse, Rat, Synthetic peptide samples. Cited in 48 publications.
Images
![Immunoprecipitation - Anti-IKB alpha (phospho S32) antibody [EPR3148] (AB92700), expandable thumbnail](https://content.abcam.com/products/images/ikb-alpha-phospho-s32-antibody-epr3148-ab92700--immunoprecipitation-img10245.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-IKB alpha (phospho S32) antibody [EPR3148] (AB92700)")
![Western blot - Anti-IKB alpha (phospho S32) antibody [EPR3148] (AB92700), expandable thumbnail](https://content.abcam.com/products/images/ikb-alpha-phospho-s32-antibody-epr3148-ab92700--western-blot-img74641.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-IKB alpha (phospho S32) antibody [EPR3148] (AB92700)")
![Western blot - Anti-IKB alpha (phospho S32) antibody [EPR3148] (AB92700), expandable thumbnail](https://content.abcam.com/products/images/ikb-alpha-phospho-s32-antibody-epr3148-ab92700--western-blot-img89128.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-IKB alpha (phospho S32) antibody [EPR3148] (AB92700)")
![Western blot - Anti-IKB alpha (phospho S32) antibody [EPR3148] (AB92700), expandable thumbnail](https://content.abcam.com/products/images/ikb-alpha-phospho-s32-antibody-epr3148-ab92700--western-blot-img23325.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-IKB alpha (phospho S32) antibody [EPR3148] (AB92700)")
![Dot Blot - Anti-IKB alpha (phospho S32) antibody [EPR3148] (AB92700), expandable thumbnail](https://content.abcam.com/products/images/ikb-alpha-phospho-s32-antibody-epr3148-ab92700--dot-blot-img6028.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-IKB alpha (phospho S32) antibody [EPR3148] (AB92700)")
Publications
Filter by
- Folia histochemica et cytobiologica 60:311-3222022Dexmedetomidine alleviates intestinal barrier dysfunction and inflammatory response in mice via suppressing TLR4/MyD88/NF-κB signaling in an experimental model of ulcerative colitis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaojin Ye,Huailiang Xu,Yuan XuPubMed 36504132
- Cell biology international 46:1927-19362022SARM suppresses glioma progression in GL261 glioma cells and regulates microglial polarization.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChun Zhou,Tianzun Li,Qian Dong,Hong Liang,Lunshan XuPubMed 35971755
- Molecules (Basel, Switzerland) 27:2022Polyphenols from the Peels of L. and Their Bioactivity of Suppressing Lipopolysaccharide-Stimulated Inflammatory Cytokines and Mediators in RAW 264.7 Cells via Activating p38 MAPK and NF-κB Signaling Pathways.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHui-Min Li,Ongher Kouye,Ding-Shan Yang,Ya-Qi Zhang,Jing-Ya Ruan,Li-Feng Han,Yi Zhang,Tao WangPubMed 35889496
- Translational cancer research 11:403-4132022Inhibition of NF-κB activation by BAY 11-7821 suppresses the proliferation and inflammation of glioma cells through inducing autophagy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKui Xie,Deming Zhou,Chao Fang,Rong Pu,Zhanpeng ZhuPubMed 35281421
- Cell cycle (Georgetown, Tex.) 21:406-4152022Ozone treatment alleviates brain injury in cerebral ischemic rats by inhibiting the NF-κB signaling pathway and autophagy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiang Zhu,Shengyang Ding,Lingshan Xu,Zhouquan WuPubMed 34985377
- CNS neuroscience & therapeutics 28:390-4002021Electroacupuncture ameliorates postoperative cognitive dysfunction and associated neuroinflammation via NLRP3 signal inhibition in aged mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLong Sun,Yue Yong,Pan Wei,Yongqiang Wang,He Li,Yalan Zhou,Wenqing Ruan,Xing Li,Jiangang SongPubMed 34951130
- Frontiers in pharmacology 12:7854032021Pterostilbene Alleviates Cholestasis by Promoting SIRT1 Activity in Hepatocytes and Macrophages.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChuanrui Ma,Jiaqing Xiang,Guixiao Huang,Yaxi Zhao,Xinyu Wang,Han Wu,Kewei Jiang,Zhen Liang,Lin Kang,Guangyan Yang,Shu YangPubMed 34899349
- Journal of thoracic disease 13:5409-54182021The role of targeted regulation of by miR-10a-3p in the development and progression of paediatric mycoplasma pneumoniae pneumonia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenhong Li,Xin Ding,Rui Zhao,Donghui Xiong,Zhiping Xie,Jing Xu,Meiling Tan,Chunyu Li,Chunfu YangPubMed 34659807
- Molecular medicine reports 24:2021Cinnamaldehyde inhibits psoriasis‑like inflammation by suppressing proliferation and inflammatory response of keratinocytes via inhibition of NF‑κB and JNK signaling pathways.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhenzhen Ding,Jingjing Liu,Huangjing Qian,Lingjian Wu,Mingfen LvPubMed 34278486
- Molecular medicine reports 23:2021Propofol alleviates inflammation and apoptosis in HCY‑induced HUVECs by inhibiting endoplasmic reticulum stress.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCunliang Ji,Hu Yi,Jing Huang,Wenzhong Zhang,Mingzhi ZhengPubMed 33760174
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Dot | WB | |
|---|---|---|---|
| Human | Tested | Expected | Tested |
| Mouse | Expected | Expected | Tested |
| Rat | Expected | Expected | Tested |
| Synthetic peptide | Not recommended | Tested | Not recommended |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20 | Notes For unpurified use at 1/10 - 1/100 |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes For unpurified use at 1/500 - 1/10,000 |
Species Mouse | Dilution info 1/1000 | Notes For unpurified use at 1/500 - 1/10,000 |
Species Rat | Dilution info 1/1000 | Notes For unpurified use at 1/500 - 1/10,000 |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Associated Products
Select an associated product type
15 products for Alternative Product
Target data
Function
Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL (RELA/p65 and NFKB1/p50) dimers in the cytoplasm by masking their nuclear localization signals (PubMed:1493333, PubMed:36651806, PubMed:7479976). On cellular stimulation by immune and pro-inflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription (PubMed:7479976, PubMed:7628694, PubMed:7796813, PubMed:7878466).
Alternative names
IKBA, MAD3, NFKBI, NFKBIA, NF-kappa-B inhibitor alpha, I-kappa-B-alpha, Major histocompatibility complex enhancer-binding protein MAD3, IkB-alpha, IkappaBalpha
Recommended products
Rabbit Recombinant Monoclonal IKB alpha phospho S32 antibody. Suitable for IP, Dot, WB and reacts with Human, Mouse, Rat, Synthetic peptide samples. Cited in 48 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR3148
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Immunoprecipitation - Anti-IKB alpha (phospho S32) antibody [EPR3148] (ab92700), expandable thumbnail](https://content.abcam.com/products/images/ikb-alpha-phospho-s32-antibody-epr3148-ab92700--immunoprecipitation-img10245.jpg "Immunoprecipitation - Anti-IKB alpha (phospho S32) antibody [EPR3148] (ab92700)")
Immunoprecipitation - Anti-IKB alpha (phospho S32) antibody [EPR3148] (ab92700)
Purified ab92700 at 1:20 dilution (1μg) immunoprecipitating IKB alpha in HeLa treated with 20ng/mL TNF-alpha for 60 minutes whole cell lysate.
Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) treated with 20ng/mL TNF-alpha for 60 minutes whole cell lysate 10μg.
Lane 2 (+): ab92700 + HeLa treated with 20ng/mL TNF-alpha for 60 minutes whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab92700 in HeLa treated with 20ng/mL TNF-alpha for 60 minutes whole cell lysate.Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 36 kDaAll lanes: Immunoprecipitation - Anti-IKB alpha (phospho S32) antibody [EPR3148] (ab92700)
Predicted band size: 35 kDa
![Western blot - Anti-IKB alpha (phospho S32) antibody [EPR3148] (ab92700), expandable thumbnail](https://content.abcam.com/products/images/ikb-alpha-phospho-s32-antibody-epr3148-ab92700--western-blot-img74641.jpg "Western blot - Anti-IKB alpha (phospho S32) antibody [EPR3148] (ab92700)")
Western blot - Anti-IKB alpha (phospho S32) antibody [EPR3148] (ab92700)
Blocking/Diluting buffer: 5% NFDM/TBSTAll lanes: Western blot - Anti-IKB alpha (phospho S32) antibody [EPR3148] (ab92700) at 1/1000 dilution
Lane 1: MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2: MCF7 (Human breast adenocarcinoma epithelial cell) treated with 20 ng/mL TNF-alpha for 8 hours whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 35 kDa
Observed band size: 36 kDa
![Western blot - Anti-IKB alpha (phospho S32) antibody [EPR3148] (ab92700), expandable thumbnail](https://content.abcam.com/products/images/ikb-alpha-phospho-s32-antibody-epr3148-ab92700--western-blot-img89128.jpg "Western blot - Anti-IKB alpha (phospho S32) antibody [EPR3148] (ab92700)")
Western blot - Anti-IKB alpha (phospho S32) antibody [EPR3148] (ab92700)
Blocking/Diluting buffer: 5% NFDM/TBSTAll lanes: Western blot - Anti-IKB alpha (phospho S32) antibody [EPR3148] (ab92700) at 1/1000 dilution
Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 15 µg
Lane 2: HeLa (Human cervix adenocarcinoma epithelial cell) treated with 20 ng/mL TNF-alpha for 5 minutes whole cell lysate at 15 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 35 kDa
Observed band size: 36 kDa
![Western blot - Anti-IKB alpha (phospho S32) antibody [EPR3148] (ab92700), expandable thumbnail](https://content.abcam.com/products/images/ikb-alpha-phospho-s32-antibody-epr3148-ab92700--western-blot-img23325.jpg "Western blot - Anti-IKB alpha (phospho S32) antibody [EPR3148] (ab92700)")
Western blot - Anti-IKB alpha (phospho S32) antibody [EPR3148] (ab92700)
Blocking/Diluting buffer and concentration 5% NFDM/TBST
All lanes: Western blot - Anti-IKB alpha (phospho S32) antibody [EPR3148] (ab92700) at 1/500 dilution
Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg
Lane 2: HeLa (Human cervix adenocarcinoma epithelial cell) treated with TNF-α at 20 ng/mL for 5 minutes. Whole cell lysates at 15 µg
Lane 3: HeLa treated with TNF-α at 20 ng/mL for 5 minutes. Whole cell lysates. Then the membrane was incubated with phosphatase. at 15 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 35 kDa
Observed band size: 36 kDa
Exposure time: 3min
![Dot Blot - Anti-IKB alpha (phospho S32) antibody [EPR3148] (ab92700), expandable thumbnail](https://content.abcam.com/products/images/ikb-alpha-phospho-s32-antibody-epr3148-ab92700--dot-blot-img6028.jpg "Dot Blot - Anti-IKB alpha (phospho S32) antibody [EPR3148] (ab92700)")
Dot Blot - Anti-IKB alpha (phospho S32) antibody [EPR3148] (ab92700)
Dot blot analysis of IKB alpha (phospho S32) phospho peptide (Lane 1) and IKB alpha non-phospho peptide (Lane 2) labeling IKB alpha (phospho S32) with unpurified ab92700 at a dilution of 1/1000. Goat Anti-Rabbit IgG H&L (HRP) ab97051 (Peroxidase conjugated goat anti-rabbit IgG) (H+L) at 1/10000 was used as the secondary antibody.
Blocking and diluting buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
![Western blot - Anti-IKB alpha (phospho S32) antibody [EPR3148] (ab92700), expandable thumbnail](https://content.abcam.com/products/images/ikb-alpha-phospho-s32-antibody-epr3148-ab92700--western-blot-img427896.jpg "Western blot - Anti-IKB alpha (phospho S32) antibody [EPR3148] (ab92700)")
Western blot - Anti-IKB alpha (phospho S32) antibody [EPR3148] (ab92700)
Blocking/Diluting buffer and concentration: 5% NFDM/TBST.
All lanes: Western blot - Anti-IKB alpha (phospho S32) antibody [EPR3148] (ab92700) at 1/1000 dilution
Lane 1: Untreated C6 (Rat glial tumor glial cell) whole cell lysate at 20 µg
Lane 2: C6 (Rat glial tumor glial cell) treated with 100 ng/mL Calyculin A for 30 minutes whole cell lysate at 20 µg
Lane 3: C6 (Rat glial tumor glial cell) treated with 100 ng/mL Calyculin A for 30 minutes whole cell lysate. Then the membrane was incubated with alkaline phosphatase at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 35 kDa
Observed band size: 36 kDa
Exposure time: 20s
![Western blot - Anti-IKB alpha (phospho S32) antibody [EPR3148] (ab92700), expandable thumbnail](https://content.abcam.com/products/images/ikb-alpha-phospho-s32-antibody-epr3148-ab92700--western-blot-img427895.jpg "Western blot - Anti-IKB alpha (phospho S32) antibody [EPR3148] (ab92700)")
Western blot - Anti-IKB alpha (phospho S32) antibody [EPR3148] (ab92700)
Blocking/Diluting buffer and concentration: 5% NFDM/TBST.
All lanes: Western blot - Anti-IKB alpha (phospho S32) antibody [EPR3148] (ab92700) at 1/1000 dilution
Lane 1: Untreated Raw264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg
Lane 2: Raw264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) treated with 50 ng/mL TNF-a and 300 ng/ml BFA for 24 hours whole cell lysate at 20 µg
Lane 3: Raw264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) treated with 50 ng/mL TNF-a and 300 ng/ml BFA for 24 hours whole cell lysate.Then the membrane was incubated with alkaline phosphatase at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 35 kDa
Observed band size: 36 kDa
Exposure time: 180s
![Western blot - Anti-IKB alpha (phospho S32) antibody [EPR3148] (ab92700), expandable thumbnail](https://content.abcam.com/products/images/ikb-alpha-phospho-s32-antibody-epr3148-ab92700--western-blot-img427896.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-IKB alpha (phospho S32) antibody [EPR3148] (ab92700)")
![Western blot - Anti-IKB alpha (phospho S32) antibody [EPR3148] (ab92700), expandable thumbnail](https://content.abcam.com/products/images/ikb-alpha-phospho-s32-antibody-epr3148-ab92700--western-blot-img427895.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-IKB alpha (phospho S32) antibody [EPR3148] (ab92700)")
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com