Rabbit Recombinant Monoclonal IKB alpha phospho S32 antibody. Carrier free. Suitable for IP, Dot, WB and reacts with Human, Mouse, Rat, Synthetic peptide samples.
Images
![Immunoprecipitation - Anti-IKB alpha (phospho S32) antibody [EPR3148] - BSA and Azide free (AB239920), expandable thumbnail](https://content.abcam.com/products/images/ikb-alpha-phospho-s32-antibody-epr3148-bsa-and-azide-free-ab239920--immunoprecipitation-img10245.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-IKB alpha (phospho S32) antibody [EPR3148] - BSA and Azide free (AB239920)")
![Western blot - Anti-IKB alpha (phospho S32) antibody [EPR3148] - BSA and Azide free (AB239920), expandable thumbnail](https://content.abcam.com/products/images/ikb-alpha-phospho-s32-antibody-epr3148-bsa-and-azide-free-ab239920--western-blot-img74641.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-IKB alpha (phospho S32) antibody [EPR3148] - BSA and Azide free (AB239920)")
![Western blot - Anti-IKB alpha (phospho S32) antibody [EPR3148] - BSA and Azide free (AB239920), expandable thumbnail](https://content.abcam.com/products/images/ikb-alpha-phospho-s32-antibody-epr3148-bsa-and-azide-free-ab239920--western-blot-img89128.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-IKB alpha (phospho S32) antibody [EPR3148] - BSA and Azide free (AB239920)")
![Dot Blot - Anti-IKB alpha (phospho S32) antibody [EPR3148] - BSA and Azide free (AB239920), expandable thumbnail](https://content.abcam.com/products/images/ikb-alpha-phospho-s32-antibody-epr3148-bsa-and-azide-free-ab239920--dot-blot-img6028.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-IKB alpha (phospho S32) antibody [EPR3148] - BSA and Azide free (AB239920)")
![Western blot - Anti-IKB alpha (phospho S32) antibody [EPR3148] - BSA and Azide free (AB239920), expandable thumbnail](https://content.abcam.com/products/images/ikb-alpha-phospho-s32-antibody-epr3148-bsa-and-azide-free-ab239920--western-blot-img427896.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-IKB alpha (phospho S32) antibody [EPR3148] - BSA and Azide free (AB239920)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Dot | WB | |
|---|---|---|---|
| Human | Tested | Expected | Tested |
| Mouse | Expected | Expected | Tested |
| Rat | Expected | Expected | Tested |
| Synthetic peptide | Not recommended | Tested | Not recommended |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Function
Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL (RELA/p65 and NFKB1/p50) dimers in the cytoplasm by masking their nuclear localization signals (PubMed:1493333, PubMed:36651806, PubMed:7479976). On cellular stimulation by immune and pro-inflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription (PubMed:7479976, PubMed:7628694, PubMed:7796813, PubMed:7878466).
Alternative names
IKBA, MAD3, NFKBI, NFKBIA, NF-kappa-B inhibitor alpha, I-kappa-B-alpha, Major histocompatibility complex enhancer-binding protein MAD3, IkB-alpha, IkappaBalpha
Recommended products
Rabbit Recombinant Monoclonal IKB alpha phospho S32 antibody. Carrier free. Suitable for IP, Dot, WB and reacts with Human, Mouse, Rat, Synthetic peptide samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR3148
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab239920 is the carrier-free version of Anti-IKB alpha (phospho S32) antibody [EPR3148] ab92700.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Immunoprecipitation - Anti-IKB alpha (phospho S32) antibody [EPR3148] - BSA and Azide free (ab239920), expandable thumbnail](https://content.abcam.com/products/images/ikb-alpha-phospho-s32-antibody-epr3148-bsa-and-azide-free-ab239920--immunoprecipitation-img10245.jpg "Immunoprecipitation - Anti-IKB alpha (phospho S32) antibody [EPR3148] - BSA and Azide free (ab239920)")
Immunoprecipitation - Anti-IKB alpha (phospho S32) antibody [EPR3148] - BSA and Azide free (ab239920)
Purified Anti-IKB alpha (phospho S32) antibody [EPR3148] ab92700 at 1:20 dilution (1μg) immunoprecipitating IKB alpha in HeLa treated with 20ng/mL TNF-alpha for 60 minutes whole cell lysate.
Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) treated with 20ng/mL TNF-alpha for 60 minutes whole cell lysate 10μg
Lane 2 (+): Anti-IKB alpha (phospho S32) antibody [EPR3148] ab92700 + HeLa treated with 20ng/mL TNF-alpha for 60 minutes whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-IKB alpha (phospho S32) antibody [EPR3148] ab92700 in HeLa treated with 20ng/mL TNF-alpha for 60 minutes whole cell lysate.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 36 kDaThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-IKB alpha (phospho S32) antibody [EPR3148] ab92700).
All lanes: Immunoprecipitation - Anti-IKB alpha (phospho S32) antibody [EPR3148] (Anti-IKB alpha (phospho S32) antibody [EPR3148] ab92700)
Predicted band size: 35 kDa
![Western blot - Anti-IKB alpha (phospho S32) antibody [EPR3148] - BSA and Azide free (ab239920), expandable thumbnail](https://content.abcam.com/products/images/ikb-alpha-phospho-s32-antibody-epr3148-bsa-and-azide-free-ab239920--western-blot-img74641.jpg "Western blot - Anti-IKB alpha (phospho S32) antibody [EPR3148] - BSA and Azide free (ab239920)")
Western blot - Anti-IKB alpha (phospho S32) antibody [EPR3148] - BSA and Azide free (ab239920)
Blocking/Diluting buffer: 5% NFDM/TBSTThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-IKB alpha (phospho S32) antibody [EPR3148] ab92700).
All lanes: Western blot - Anti-IKB alpha (phospho S32) antibody [EPR3148] (Anti-IKB alpha (phospho S32) antibody [EPR3148] ab92700) at 1/1000 dilution
Lane 1: MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2: MCF7 (Human breast adenocarcinoma epithelial cell) treated with 20 ng/mL TNF-alpha for 8 hours whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 35 kDa
Observed band size: 36 kDa
![Western blot - Anti-IKB alpha (phospho S32) antibody [EPR3148] - BSA and Azide free (ab239920), expandable thumbnail](https://content.abcam.com/products/images/ikb-alpha-phospho-s32-antibody-epr3148-bsa-and-azide-free-ab239920--western-blot-img89128.jpg "Western blot - Anti-IKB alpha (phospho S32) antibody [EPR3148] - BSA and Azide free (ab239920)")
Western blot - Anti-IKB alpha (phospho S32) antibody [EPR3148] - BSA and Azide free (ab239920)
Blocking/Diluting buffer: 5% NFDM/TBSTThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-IKB alpha (phospho S32) antibody [EPR3148] ab92700).
All lanes: Western blot - Anti-IKB alpha (phospho S32) antibody [EPR3148] (Anti-IKB alpha (phospho S32) antibody [EPR3148] ab92700) at 1/1000 dilution
Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 15 µg
Lane 2: HeLa (Human cervix adenocarcinoma epithelial cell) treated with 20 ng/mL TNF-alpha for 5 minutes whole cell lysate at 15 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 35 kDa
Observed band size: 36 kDa
![Dot Blot - Anti-IKB alpha (phospho S32) antibody [EPR3148] - BSA and Azide free (ab239920), expandable thumbnail](https://content.abcam.com/products/images/ikb-alpha-phospho-s32-antibody-epr3148-bsa-and-azide-free-ab239920--dot-blot-img6028.jpg "Dot Blot - Anti-IKB alpha (phospho S32) antibody [EPR3148] - BSA and Azide free (ab239920)")
Dot Blot - Anti-IKB alpha (phospho S32) antibody [EPR3148] - BSA and Azide free (ab239920)
Dot blot analysis of IKB alpha (phospho S32) phospho peptide (Lane 1) and IKB alpha non-phospho peptide (Lane 2) labeling IKB alpha (phospho S32) with unpurified Anti-IKB alpha (phospho S32) antibody [EPR3148] ab92700 at a dilution of 1/1000. Goat Anti-Rabbit IgG H&L (HRP) ab97051 (Peroxidase conjugated goat anti-rabbit IgG) (H+L) at 1/10000 was used as the secondary antibody.
Blocking and diluting buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-IKB alpha (phospho S32) antibody [EPR3148] ab92700).
![Western blot - Anti-IKB alpha (phospho S32) antibody [EPR3148] - BSA and Azide free (ab239920), expandable thumbnail](https://content.abcam.com/products/images/ikb-alpha-phospho-s32-antibody-epr3148-bsa-and-azide-free-ab239920--western-blot-img427896.jpg "Western blot - Anti-IKB alpha (phospho S32) antibody [EPR3148] - BSA and Azide free (ab239920)")
Western blot - Anti-IKB alpha (phospho S32) antibody [EPR3148] - BSA and Azide free (ab239920)
Blocking/Diluting buffer and concentration: 5% NFDM/TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-IKB alpha (phospho S32) antibody [EPR3148] ab92700).
All lanes: Western blot - Anti-IKB alpha (phospho S32) antibody [EPR3148] (Anti-IKB alpha (phospho S32) antibody [EPR3148] ab92700) at 1/1000 dilution
Lane 1: Untreated C6 (Rat glial tumor glial cell) whole cell lysate at 20 µg
Lane 2: C6 (Rat glial tumor glial cell) treated with 100 ng/mL Calyculin A for 30 minutes whole cell lysate at 20 µg
Lane 3: C6 (Rat glial tumor glial cell) treated with 100 ng/mL Calyculin A for 30 minutes whole cell lysate. Then the membrane was incubated with alkaline phosphatase at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 35 kDa
Observed band size: 36 kDa
Exposure time: 20s
![Western blot - Anti-IKB alpha (phospho S32) antibody [EPR3148] - BSA and Azide free (ab239920), expandable thumbnail](https://content.abcam.com/products/images/ikb-alpha-phospho-s32-antibody-epr3148-bsa-and-azide-free-ab239920--western-blot-img427895.jpg "Western blot - Anti-IKB alpha (phospho S32) antibody [EPR3148] - BSA and Azide free (ab239920)")
Western blot - Anti-IKB alpha (phospho S32) antibody [EPR3148] - BSA and Azide free (ab239920)
Blocking/Diluting buffer and concentration: 5% NFDM/TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-IKB alpha (phospho S32) antibody [EPR3148] ab92700).
All lanes: Western blot - Anti-IKB alpha (phospho S32) antibody [EPR3148] (Anti-IKB alpha (phospho S32) antibody [EPR3148] ab92700) at 1/1000 dilution
Lane 1: Untreated Raw264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg
Lane 2: Raw264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) treated with 50 ng/mL TNF-a and 300 ng/ml BFA for 24 hours whole cell lysate at 20 µg
Lane 3: Raw264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) treated with 50 ng/mL TNF-a and 300 ng/ml BFA for 24 hours whole cell lysate.Then the membrane was incubated with alkaline phosphatase at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 35 kDa
Observed band size: 36 kDa
Exposure time: 180s
![Western blot - Anti-IKB alpha (phospho S32) antibody [EPR3148] - BSA and Azide free (ab239920), expandable thumbnail](https://content.abcam.com/products/images/ikb-alpha-phospho-s32-antibody-epr3148-bsa-and-azide-free-ab239920--western-blot-img427895.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-IKB alpha (phospho S32) antibody [EPR3148] - BSA and Azide free (ab239920)")
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com