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Anti-IKB alpha (phospho S36) antibody [EPR6235(2)] (ab133462) is a rabbit monoclonal antibody that is used to detect IKB alpha in Western Blot, Flow Cytometry (Intra), Flow Cytometry. Suitable for Human, Mouse, Rat samples.



- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency

-Over 230 publications


Images

Western blot - Anti-IKB alpha (phospho S36) antibody [EPR6235(2)] (AB133462), expandable thumbnail
  • Western blot - Anti-IKB alpha (phospho S36) antibody [EPR6235(2)] (AB133462), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-IKB alpha (phospho S36) antibody [EPR6235(2)] (AB133462), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBFlow Cyt (Intra)
Human
Tested
Tested
Mouse
Tested
Expected
Rat
Tested
Expected

Tested
Tested

Species
Mouse
Dilution info
1/10000
Notes

-

Species
Rat
Dilution info
1/10000
Notes

-

Species
Human
Dilution info
1/10000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

15 products for Alternative Product

Target data

Function

Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL (RELA/p65 and NFKB1/p50) dimers in the cytoplasm by masking their nuclear localization signals (PubMed:1493333, PubMed:36651806, PubMed:7479976). On cellular stimulation by immune and pro-inflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription (PubMed:7479976, PubMed:7628694, PubMed:7796813, PubMed:7878466).

Alternative names

Recommended products

Anti-IKB alpha (phospho S36) antibody [EPR6235(2)] (ab133462) is a rabbit monoclonal antibody that is used to detect IKB alpha in Western Blot, Flow Cytometry (Intra), Flow Cytometry. Suitable for Human, Mouse, Rat samples.



- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency

-Over 230 publications

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR6235(2)
Purification technique
Affinity purification Protein A
Specificity

This antibody detects IKB alpha only when phosphorylated at Serine 36. Stimulation may be required to allow detection of the phosphorylated protein due to low levels of endogenous expression. Please see images below for recommended treatment conditions and positive controls.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C

Notes

What is this antibody validated in?


Anti-IKB alpha (phospho S36) antibody [EPR6235(2)] (ab133462) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt) in Human, Mouse, Rat samples.

What is the molecular weight of IKB alpha?


Anti-IKB alpha (phospho S36) [EPR6235(2)] (ab133462) specifically detects a band for IKB alpha (UniProt: P25963) at a molecular weight of 35kDa.

Trusted by the scientific community


Anti-IKB alpha (phospho S36) [EPR6235(2)] (ab133462) was first used in a scientific publication in 2012 and has been cited over 230 times in peer-reviewed journals.

Trial sizes available!


Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products


We have a range of other formats of antibody clone [EPR6235(2)] also available for your convenience:
ab133462, Carrier free - Anti-IKB alpha (phospho S36) antibody [EPR6235(2)] - BSA and Azide free ab240059, Alexa Fluor® 488 - Alexa Fluor® 488 Anti-IKB alpha (phospho S36) antibody [EPR6235(2)] ab317068, Alexa Fluor® 647 - Alexa Fluor® 647 Anti-IKB alpha (phospho S36) antibody [EPR6235(2)] ab317485



Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-IKB alpha (phospho S36) antibody [EPR6235(2)] (ab133462), expandable thumbnail

    Western blot - Anti-IKB alpha (phospho S36) antibody [EPR6235(2)] (ab133462)

    All lanes: Western blot - Anti-IKB alpha (phospho S36) antibody [EPR6235(2)] (ab133462) at 1/1000 dilution

    Lane 1: NIH/3T3 (Mouse embryonic fibroblast) starved for 24 hours at 15 µg

    Lane 2: NIH/3T3 (Mouse embryonic fibroblast) starved for 24 hours then treated with 100nM Calyculin A for 30 minutes at 15 µg

    Lane 3: NIH/3T3 (Mouse embryonic fibroblast) starved for 24 hours then treated with 100nM Calyculin A for 30 minutes, then the menbrane treated with Alkaline Phosphatase for 1 hour at 15 µg

    Lane 4: Untreated C6 (Rat glial tumor glial cell) at 15 µg

    Lane 5: C6 (Rat glial tumor glial cell) treated with 100ng/ml Calyculin A for 60 minutes at 15 µg

    Lane 6: C6 (Rat glial tumor glial cell) treated with 100ng/ml Calyculin A for 60 minutes, then the membrane treated with Alkaline Phosphatase for 1 hour at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 35 kDa

    Observed band size: 40 kDa

  • Western blot - Anti-IKB alpha (phospho S36) antibody [EPR6235(2)] (ab133462), expandable thumbnail

    Western blot - Anti-IKB alpha (phospho S36) antibody [EPR6235(2)] (ab133462)

    All lanes: Western blot - Anti-IKB alpha (phospho S36) antibody [EPR6235(2)] (ab133462) at 1/1000 dilution

    Lane 1: Untreated THP-1 (Human monocytic leukemia monocyte) at 15 µg

    Lane 2: THP-1 (Human monocytic leukemia monocyte) treated with 100ng/ml IFN-gamma for 24 hours, then 300ng/ml Brefeldin A added and treated for another 20 hours at 15 µg

    Lane 3: THP-1 (Human monocytic leukemia monocyte) treated with 100ng/ml IFN-gamma for 24 hours, then 300ng/ml Brefeldin A added and treated for another 20 hours, then the menbrane treated with Alkaline Phosphatase for 1 hour at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 35 kDa

    Observed band size: 40 kDa

  • Flow Cytometry (Intracellular) - Anti-IKB alpha (phospho S36) antibody [EPR6235(2)] (ab133462), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-IKB alpha (phospho S36) antibody [EPR6235(2)] (ab133462)

    Intracellular flow cytometric analysis of 4% Paraformaldehyde fixed 90% Methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) treated with 1% FBS for 3 hours then 100 nM Calyculin A for 10 minutes, labelling IKB alpha (phospho S36) with ab133462 at 1/100 dilution (1μg/ml) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 was used as the secondary antibody. Untreated control: Green

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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