AB133462

Anti-IKB alpha (phospho S36) antibody [EPR6235(2)]

Name: Anti-IKB alpha (phospho S36) antibody [EPR6235(2)] (ab133462) | Abcam
Brand: Abcam Limited
SKU: AB133462
Price: 170 USD
Availability: InStock
Rating: 4 (1 reviews)

20ul selling size
RabMAb
Recombinant
What is this?

(1 Review)

(340 Publications)

Anti-IKB alpha (phospho S36) antibody [EPR6235(2)] (ab133462) is a rabbit monoclonal antibody detecting IKB alpha in Western Blot, Flow Cytometry (Intra). Suitable for Human, Mouse, Rat.

- Biophysical QC for unrivalled batch-batch consistency
- Over 230 publications

View Alternative Names

IKBA, MAD3, NFKBI, NFKBIA, NF-kappa-B inhibitor alpha, I-kappa-B-alpha, Major histocompatibility complex enhancer-binding protein MAD3, IkB-alpha, IkappaBalpha

3 Images

Flow Cytometry (Intracellular) - Anti-IKB alpha (phospho S36) antibody [EPR6235(2)] (AB133462)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-IKB alpha (phospho S36) antibody [EPR6235(2)] (AB133462)

Intracellular flow cytometric analysis of 4% Paraformaldehyde fixed 90% Methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) treated with 1% FBS for 3 hours then 100 nM Calyculin A for 10 minutes, labelling IKB alpha (phospho S36) with ab133462 at 1/100 dilution (1μg/ml) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 was used as the secondary antibody. Untreated control : Green

Lab

Western blot - Anti-IKB alpha (phospho S36) antibody [EPR6235(2)] (AB133462)

All lanes:

Western blot - Anti-IKB alpha (phospho S36) antibody [EPR6235(2)] (ab133462) at 1/1000 dilution

Lane 1:

Untreated THP-1 (Human monocytic leukemia monocyte) at 15 µg

Lane 2:

THP-1 (Human monocytic leukemia monocyte) treated with 100ng/ml IFN-gamma for 24 hours, then 300ng/ml Brefeldin A added and treated for another 20 hours at 15 µg

Lane 3:

THP-1 (Human monocytic leukemia monocyte) treated with 100ng/ml IFN-gamma for 24 hours, then 300ng/ml Brefeldin A added and treated for another 20 hours, then the menbrane treated with Alkaline Phosphatase for 1 hour at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 35 kDa

Observed band size: 40 kDa

false

Lab

Western blot - Anti-IKB alpha (phospho S36) antibody [EPR6235(2)] (AB133462)

All lanes:

Western blot - Anti-IKB alpha (phospho S36) antibody [EPR6235(2)] (ab133462) at 1/1000 dilution

Lane 1:

NIH/3T3 (Mouse embryonic fibroblast) starved for 24 hours at 15 µg

Lane 2:

NIH/3T3 (Mouse embryonic fibroblast) starved for 24 hours then treated with 100nM Calyculin A for 30 minutes at 15 µg

Lane 3:

NIH/3T3 (Mouse embryonic fibroblast) starved for 24 hours then treated with 100nM Calyculin A for 30 minutes, then the menbrane treated with Alkaline Phosphatase for 1 hour at 15 µg

Lane 4:

Untreated C6 (Rat glial tumor glial cell) at 15 µg

Lane 5:

C6 (Rat glial tumor glial cell) treated with 100ng/ml Calyculin A for 60 minutes at 15 µg

Lane 6:

C6 (Rat glial tumor glial cell) treated with 100ng/ml Calyculin A for 60 minutes, then the membrane treated with Alkaline Phosphatase for 1 hour at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 35 kDa

Observed band size: 40 kDa

false

Carrier free

Anti-IKB alpha (phospho S36) antibody [EPR6235(2)] - BSA and Azide free
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-IKB alpha (phospho S36) antibody [EPR6235(2)]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-IKB alpha (phospho S36) antibody [EPR6235(2)]
578 PE

PE Anti-IKB alpha (phospho S36) antibody [EPR6235(2)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6235(2)

Isotype

IgG

Carrier free

Reacts with

Human, Mouse, Rat

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody detects IKB alpha only when phosphorylated at Serine 36. Stimulation may be required to allow detection of the phosphorylated protein due to low levels of endogenous expression. Please see images below for recommended treatment conditions and positive controls.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100", "FlowCytIntra-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-IKB alpha (phospho S36) antibody [EPR6235(2)] (ab133462) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt) in Human, Mouse, Rat samples.

What is the molecular weight of IKB alpha?
Anti-IKB alpha (phospho S36) [EPR6235(2)] (ab133462) specifically detects a band for IKB alpha (UniProt: P25963) at a molecular weight of 35kDa.

Trusted by the scientific community
Anti-IKB alpha (phospho S36) [EPR6235(2)] (ab133462) was first used in a scientific publication in 2012 and has been cited over 230 times in peer-reviewed journals.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR6235(2)] also available for your convenience: ab133462, Carrier free - ab240059, Alexa Fluor® 488 - ab317068, Alexa Fluor® 647 - ab317485

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL (RELA/p65 and NFKB1/p50) dimers in the cytoplasm by masking their nuclear localization signals (PubMed : 1493333, PubMed : 36651806, PubMed : 7479976). On cellular stimulation by immune and pro-inflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription (PubMed : 7479976, PubMed : 7628694, PubMed : 7796813, PubMed : 7878466).

See full target information NFKBIA phospho S36

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Publications (340)

Recent publications for all applications. Explore the full list and refine your search

Journal of cell communication and signaling 19:e70048 PubMed41040887

2025

Targeting the PI3K/Akt/NF-κB axis: Cluster of differentiation 5-like-mediated immunometabolic regulation of macrophage polarization in abdominal aortic aneurysm.

Applications

Unspecified application

Species

Unspecified reactive species

Hemoren Yi,Nan Liu,Zhengyang Wu,Lei Li,Tingting Li,Qixiang Liu,Man Duan,Taihu Wan

Scientific reports 15:33159 PubMed41006814

2025

Pedunculoside alleviates lipopolysaccharide-induced acute lung injury/acute respiratory distress syndrome by inhibiting NF-κB pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Yang,Zhi Xu,Qi Li,Guansong Wang,Jiancheng Xu

Journal of translational medicine 23:762 PubMed40640887

2025

Gut microbiota dysbiosis exacerbates heart failure by the LPS-TLR4/NF-κB signalling axis: mechanistic insights and therapeutic potential of TLR4 inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Chunlei Zhang,Xiaodong Teng,Qiuhang Cao,Yanyan Deng,Mo Yang,Lei Wang,Daorong Rui,Xiu Ling,Cao Wei,Yue Chen,Dasheng Lu,Hongxiang Zhang

International journal of molecular sciences 26: PubMed40565375

2025

Isostrictiniin Alleviates LPS-Induced Acute Lung Injury via the Regulation of the Keap1-Nrf2/HO-1 and MAPK/NF-κB Signaling Pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Wanting Ding,Yuan Sun,Wulipan Tuohudaali,Chenyang Li,Yuhan Yao,Jun Zhao

American journal of translational research 17:3307-3321 PubMed40535652

2025

Luteolin alleviates Herpes Simplex Keratitis by inhibiting inflammatory responses via suppressing the PTGS2/NF-κB signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xudong Zhao,Zhichao Ren,Dingwen Cao,Yusen Huang

Molecular neurobiology 62:13430-13448 PubMed40536592

2025

VISTA Alleviates Microglia-Mediated Neuroinflammation After Cerebral Ischemia-Reperfusion Injury via Regulating ACOD1/Itaconic Acid Metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Yilei Sun,Dan Liu,Yanchen Liu,Lijun Chi

IBRO neuroscience reports 18:783-793 PubMed40510292

2025

Ginsenoside C-K inhibits Aβ oligomer-induced Alzheimer's disease pathology progression by regulating microglia-neuron interactions.

Applications

Unspecified application

Species

Unspecified reactive species

Chenghu Xie,Cunxin Zhang,Kefeng Zhang,Shanshan Zhang

Scientific reports 15:18912 PubMed40442166

2025

Uncovering the molecular mechanisms of tonifying kidney and activating blood Decoction against myocardial fibrosis using network Pharmacology and experimental validation.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Xu,Yanping Bi,Yetao Ju,Wenhao Yin,Shujun Zhao,Yan Zhang,Xin Zhao

Folia histochemica et cytobiologica 63:79-87 PubMed40421818

2025

Nintedanib alleviates hyperoxia-induced lung injury via targeting NF-κB signalling pathway in rat model of bronchopulmonary dysplasia.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Hao,Yuping Li,Junhui Li,Zirui Guo,Zhi Yang,Wei Lu

International journal of molecular sciences 26: PubMed40362261

2025

Restraint Stress Disrupted Intestinal Homeostasis via 5-HT/HTR7/Wnt/β-Catenin/NF-kB Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Jiayu Yu,Zixu Wang,Yaoxing Chen,Yulan Dong

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-IKB alpha (phospho S36) antibody [EPR6235(2)]

Flow Cytometry (Intracellular) - Anti-IKB alpha (phospho S36) antibody [EPR6235(2)] (AB133462)

Western blot - Anti-IKB alpha (phospho S36) antibody [EPR6235(2)] (AB133462)

Western blot - Anti-IKB alpha (phospho S36) antibody [EPR6235(2)] (AB133462)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (340)

Product promise