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Rabbit Recombinant Monoclonal IKB alpha phospho S36 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples.

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Images

Western blot - Anti-IKB alpha (phospho S36) antibody [EPR6235(2)] - BSA and Azide free (AB240059), expandable thumbnail
  • Western blot - Anti-IKB alpha (phospho S36) antibody [EPR6235(2)] - BSA and Azide free (AB240059), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-IKB alpha (phospho S36) antibody [EPR6235(2)] - BSA and Azide free (AB240059), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBFlow Cyt (Intra)
Human
Tested
Tested
Mouse
Tested
Expected
Rat
Tested
Expected

Tested
Tested

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

15 products for Alternative Product

Target data

Function

Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL (RELA/p65 and NFKB1/p50) dimers in the cytoplasm by masking their nuclear localization signals (PubMed:1493333, PubMed:36651806, PubMed:7479976). On cellular stimulation by immune and pro-inflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription (PubMed:7479976, PubMed:7628694, PubMed:7796813, PubMed:7878466).

Alternative names

IKBA, MAD3, NFKBI, NFKBIA, NF-kappa-B inhibitor alpha, I-kappa-B-alpha, Major histocompatibility complex enhancer-binding protein MAD3, IkB-alpha, IkappaBalpha

Recommended products

Rabbit Recombinant Monoclonal IKB alpha phospho S36 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR6235(2)
Purification technique
Affinity purification Protein A
Specificity

This antibody detects IKB alpha only when phosphorylated at Serine 36. Stimulation may be required to allow detection of the phosphorylated protein due to low levels of endogenous expression. Please see images below for recommended treatment conditions and positive controls.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab240059 is the carrier-free version of Anti-IKB alpha (phospho S36) antibody [EPR6235(2)] ab133462.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-IKB alpha (phospho S36) antibody [EPR6235(2)] - BSA and Azide free (ab240059), expandable thumbnail

    Western blot - Anti-IKB alpha (phospho S36) antibody [EPR6235(2)] - BSA and Azide free (ab240059)

    This data was developed using Anti-IKB alpha (phospho S36) antibody [EPR6235(2)] ab133462 the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-IKB alpha (phospho S36) antibody [EPR6235(2)] (Anti-IKB alpha (phospho S36) antibody [EPR6235(2)] ab133462) at 1/1000 dilution

    Lane 1: NIH/3T3 (Mouse embryonic fibroblast) starved for 24 hours at 15 µg

    Lane 2: NIH/3T3 (Mouse embryonic fibroblast) starved for 24 hours then treated with 100nM Calyculin A for 30 minutes at 15 µg

    Lane 3: NIH/3T3 (Mouse embryonic fibroblast) starved for 24 hours then treated with 100nM Calyculin A for 30 minutes, then the menbrane treated with Alkaline Phosphatase for 1 hour at 15 µg

    Lane 4: Untreated C6 (Rat glial tumor glial cell) at 15 µg

    Lane 5: C6 (Rat glial tumor glial cell) treated with 100ng/ml Calyculin A for 60 minutes at 15 µg

    Lane 6: C6 (Rat glial tumor glial cell) treated with 100ng/ml Calyculin A for 60 minutes, then the membrane treated with Alkaline Phosphatase for 1 hour at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 35 kDa

    Observed band size: 40 kDa

  • Western blot - Anti-IKB alpha (phospho S36) antibody [EPR6235(2)] - BSA and Azide free (ab240059), expandable thumbnail

    Western blot - Anti-IKB alpha (phospho S36) antibody [EPR6235(2)] - BSA and Azide free (ab240059)

    This data was developed using Anti-IKB alpha (phospho S36) antibody [EPR6235(2)] ab133462 the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-IKB alpha (phospho S36) antibody [EPR6235(2)] (Anti-IKB alpha (phospho S36) antibody [EPR6235(2)] ab133462) at 1/1000 dilution

    Lane 1: Untreated THP-1 (Human monocytic leukemia monocyte) at 15 µg

    Lane 2: THP-1 (Human monocytic leukemia monocyte) treated with 100ng/ml IFN-gamma for 24 hours, then 300ng/ml Brefeldin A added and treated for another 20 hours at 15 µg

    Lane 3: THP-1 (Human monocytic leukemia monocyte) treated with 100ng/ml IFN-gamma for 24 hours, then 300ng/ml Brefeldin A added and treated for another 20 hours, then the menbrane treated with Alkaline Phosphatase for 1 hour at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 35 kDa

    Observed band size: 40 kDa

  • Flow Cytometry (Intracellular) - Anti-IKB alpha (phospho S36) antibody [EPR6235(2)] - BSA and Azide free (ab240059), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-IKB alpha (phospho S36) antibody [EPR6235(2)] - BSA and Azide free (ab240059)

    This data was developed using Anti-IKB alpha (phospho S36) antibody [EPR6235(2)] ab133462 the same antibody clone in a different buffer formulation.

    Intracellular flow cytometric analysis of 4% Paraformaldehyde fixed 90% Methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) treated with 1% FBS for 3 hours then 100 nM Calyculin A for 10 minutes labelling IKB alpha (phospho S36) with Anti-IKB alpha (phospho S36) antibody [EPR6235(2)] ab133462 at 1/100 dilution (1μg/ml) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 was used as the secondary antibody. Untreated control: Green

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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