AB24784

Anti-IKB alpha (phospho Y305) antibody

Name: Anti-IKB alpha (phospho Y305) antibody (ab24784) | Abcam
Brand: Abcam Limited
SKU: AB24784
Rating: 3 (1 reviews)

(1 Review)

(3 Publications)

Rabbit Polyclonal IKB alpha phospho Y305 antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human NFKBIA phospho Y305 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

IKBA, MAD3, NFKBI, NFKBIA, NF-kappa-B inhibitor alpha, I-kappa-B-alpha, Major histocompatibility complex enhancer-binding protein MAD3, IkB-alpha, IkappaBalpha

1 Images

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Western blot - Anti-IKB alpha (phospho Y305) antibody (AB24784)

Western blot analysis of A431 cells treated with pervanadate (1 mM) for 30 minutes (30 ug/lane). Blots were probed with anti-IkappaBalpha (lane 1), anti-phospho-IkappaBalpha (Tyr-42) (ab24783; lanes 2-5), or anti-phospho-IkappaBalpha (Tyr-305) (ab24784; lanes 6-9). Both anti-phospho-IkappaBalpha (Tyr-42) and anti-phospho-IkappaBalpha (Tyr-305) were used in the presence of no blocking peptide (lane 2 & 6), phospho-IkappaBalpha (Tyr-42) peptide (lane 3 & 8), phospho-IkappaBalpha (Tyr-305) peptide (lane 4 & 7), or BSA conjugated to phospho-tyrosine (lane 5 & 9). Peptides and BSA-pTyr were used at 1 ug/ml.

Lane 1:

Western blot - Anti-IKB alpha (phospho Y305) antibody (ab24784)

Lane 1:

Western blot - Anti-IKB alpha (phospho Y42) antibody (<a href='/en-us/products/primary-antibodies/ikb-alpha-phospho-y42-antibody-ab24783'>ab24783</a>)

Predicted band size: 35 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human NFKBIA phospho Y305 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P25963

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

This antibody was cross-adsorbed to phospho-tyrosine coupled to agarose then affinity purified using phospho IKB alpha (Tyr-305) peptide.

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL (RELA/p65 and NFKB1/p50) dimers in the cytoplasm by masking their nuclear localization signals (PubMed : 1493333, PubMed : 36651806, PubMed : 7479976). On cellular stimulation by immune and pro-inflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription (PubMed : 7479976, PubMed : 7628694, PubMed : 7796813, PubMed : 7878466).

See full target information NFKBIA phospho Y305

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 24: PubMed34490475

2021

Remote ischemic post‑conditioning alleviates ischemia/reperfusion‑induced intestinal injury via the ERK signaling pathway‑mediated RAGE/HMGB axis.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Mi,Nan Zhang,Jiyun Wan,Ming Cheng,Jianping Liao,Xiao Zheng

International urology and nephrology 53:2409-2419 PubMed34089461

2021

LncRNA PVT1 accelerates LPS-induced septic acute kidney injury through targeting miR-17-5p and regulating NF-κB pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wensheng Yuan,Xiaoqing Xiong,Jinlong Du,Qi Fan,Rong Wang,Xia Zhang

Oncology reports 45: PubMed33760159

2021

MicroRNA‑129 inhibits colorectal cancer cell proliferation, invasion and epithelial‑to‑mesenchymal transition by targeting SOX4.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiping Chen,Tianyu Zhong,Jinghua Zhong,Yang Tang,Baodian Ling,Lanfeng Wang

View all publications

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Product promise

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