AB24783

Anti-IKB alpha (phospho Y42) antibody

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(24 Publications)

Rabbit Polyclonal IKB alpha phospho Y42 antibody. Suitable for WB and reacts with Human samples. Cited in 24 publications. Immunogen corresponding to Synthetic Peptide within Human NFKBIA phospho Y42 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

IKBA, MAD3, NFKBI, NFKBIA, NF-kappa-B inhibitor alpha, I-kappa-B-alpha, Major histocompatibility complex enhancer-binding protein MAD3, IkB-alpha, IkappaBalpha

1 Images

Western blot - Anti-IKB alpha (phospho Y42) antibody (AB24783)

WB

Unknown

Western blot - Anti-IKB alpha (phospho Y42) antibody (AB24783)

Western blot analysis of A431 cells treated with pervanadate (1 mM) for 30 minutes (30 ug/lane). Blots were probed with anti-IkappaBalpha (lane 1), anti-phospho-IkappaBalpha (Tyr-42) (ab24783; lanes 2-5), or anti-phospho-IkappaBalpha (Tyr-305) (ab24784; lanes 6-9). Both anti-phospho-IkappaBalpha (Tyr-42) and anti-phospho-IkappaBalpha (Tyr-305) were used in the presence of no blocking peptide (lane 2 & 6), phospho-IkappaBalpha (Tyr-42) peptide (lane 3 & 8), phospho-IkappaBalpha (Tyr-305) peptide (lane 4 & 7), or BSA conjugated to phospho-tyrosine (lane 5 & 9). Peptides and BSA-pTyr were used at 1 ug/ml.

Lane 1:

Western blot - Anti-IKB alpha (phospho Y305) antibody (<a href='/en-us/products/primary-antibodies/ikb-alpha-phospho-y305-antibody-ab24784'>ab24784</a>)

Lane 1:

Western blot - Anti-IKB alpha (phospho Y42) antibody (ab24783)

Predicted band size: 35 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

Immunogen

Synthetic Peptide within Human NFKBIA phospho Y42 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Specificity

There may be some homology to unrelated proteins with a conserved tyrosine phosphorylation motif. It is recommended that this antibody be used after immunoprecipitation with an IKB alpha specific antibody.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }

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Product details

We do not have concentration information for this antibody. It is optimized to work at the dilutions stated on the datasheet.

Useful in detection of phosphorylated IKB alpha (Tyr-42).

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Cross absorbed to phospho-tyrosine coupled to agarose then affinity purified using phospho-IKB alpha (Tyr-42) peptide, without carrier.

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL (RELA/p65 and NFKB1/p50) dimers in the cytoplasm by masking their nuclear localization signals (PubMed : 1493333, PubMed : 36651806, PubMed : 7479976). On cellular stimulation by immune and pro-inflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription (PubMed : 7479976, PubMed : 7628694, PubMed : 7796813, PubMed : 7878466).

See full target information NFKBIA phospho Y42

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Publications (24)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 24: PubMed34490475

2021

Remote ischemic post‑conditioning alleviates ischemia/reperfusion‑induced intestinal injury via the ERK signaling pathway‑mediated RAGE/HMGB axis.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Mi,Nan Zhang,Jiyun Wan,Ming Cheng,Jianping Liao,Xiao Zheng

Molecular medicine reports 22:5313-5325 PubMed33174011

2020

Knockdown of exosome‑mediated lnc‑PVT1 alleviates lipopolysaccharide‑induced osteoarthritis progression by mediating the HMGB1/TLR4/NF‑κB pathway via miR‑93‑5p.

Applications

Unspecified application

Species

Unspecified reactive species

Yong Meng,Siqiang Qiu,Long Sun,Jinliang Zuo

Bioengineered 11:1047-1057 PubMed32970961

2020

Silencing cyclophilin A improves insulin secretion, reduces cell apoptosis, and alleviates inflammation as well as oxidant stress in high glucose-induced pancreatic β-cells via MAPK/NF-kb signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Tangying Li,Huibiao Quan,Huachuan Zhang,Leweihua Lin,Qianying Ou,Kaining Chen

BioFactors (Oxford, England) 46:813-820 PubMed32525617

2020

LncRNA H19 aggravates TNF-α-induced inflammatory injury via TAK1 pathway in MH7A cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jialiang Yang,Yixuan Li,Lili Wang,Zhenchun Zhang,Zunzhong Li,Qian Jia

Life sciences 257:117889 PubMed32502541

2020

Taurine attenuates liver autophagy and injury of offspring in gestational diabetic mellitus rats.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Luo,Yue Tian,Chunrong Zhao

Life sciences 254:117742 PubMed32360619

2020

Baicalin attenuates fibrogenic process in human renal proximal tubular cells (HK-2) exposed to diabetic milieu.

Applications

Unspecified application

Species

Unspecified reactive species

Jung Eun Nam,So Yeon Jo,Chul Woo Ahn,Yu Sik Kim

BioFactors (Oxford, England) 46:831-838 PubMed32027070

2020

MicroRNA-31 weakens cisplatin resistance of medulloblastoma cells via NF-κB and PI3K/AKT pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Ma,Wei Cao,Meili Ding

Cell cycle (Georgetown, Tex.) 19:207-217 PubMed31876239

2019

Swainsonine protects H9c2 cells against lipopolysaccharide-induced apoptosis and inflammatory injury via down-regulating miR-429.

Applications

Unspecified application

Species

Unspecified reactive species

Hongyan Lv,Su Zhang,Xiaohong Hao

Molecular medicine reports 21:123-130 PubMed31746402

2019

Sevoflurane attenuates brain damage through inhibiting autophagy and apoptosis in cerebral ischemia‑reperfusion rats.

Applications

Unspecified application

Species

Unspecified reactive species

Cun-Xian Shi,Jin Jin,Xue-Qin Wang,Teng Song,Guang-Hong Li,Ke-Zhong Li,Jia-Hai Ma

Experimental and therapeutic medicine 18:4406-4412 PubMed31772635

2019

Chrysophanol alleviates myocardial injury in diabetic mice by regulating the SIRT1/HMGB1/NF-κB signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Xue,Jing Zhao,Aibin Zheng,Lin Li,Huaqin Chen,Wenjuan Tu,Ning Zhang,Zhangbin Yu,Qiuwei Wang,Meng Gu

View all publications

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