Rabbit Polyclonal IKB alpha phospho Y42 antibody. Suitable for WB and reacts with Human samples. Cited in 21 publications. Immunogen corresponding to Synthetic Peptide within Human NFKBIA phospho Y42 conjugated to Keyhole Limpet Haemocyanin.
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- Molecular medicine reports 24:2021Remote ischemic post‑conditioning alleviates ischemia/reperfusion‑induced intestinal injury via the ERK signaling pathway‑mediated RAGE/HMGB axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLei Mi,Nan Zhang,Jiyun Wan,Ming Cheng,Jianping Liao,Xiao ZhengPubMed 34490475
- Molecular medicine reports 22:5313-53252020Knockdown of exosome‑mediated lnc‑PVT1 alleviates lipopolysaccharide‑induced osteoarthritis progression by mediating the HMGB1/TLR4/NF‑κB pathway via miR‑93‑5p.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYong Meng et. al.PubMed 33174011
- Bioengineered 11:1047-10572020Silencing cyclophilin A improves insulin secretion, reduces cell apoptosis, and alleviates inflammation as well as oxidant stress in high glucose-induced pancreatic β-cells via MAPK/NF-kb signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTangying Li,Huibiao Quan,Huachuan Zhang,Leweihua Lin,Qianying Ou,Kaining ChenPubMed 32970961
- Life sciences 257:1178892020Taurine attenuates liver autophagy and injury of offspring in gestational diabetic mellitus rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYing Luo et. al.PubMed 32502541
- Life sciences 254:1177422020Baicalin attenuates fibrogenic process in human renal proximal tubular cells (HK-2) exposed to diabetic milieu.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJung Eun Nam et. al.PubMed 32360619
- Cell cycle (Georgetown, Tex.) 19:207-2172019Swainsonine protects H9c2 cells against lipopolysaccharide-induced apoptosis and inflammatory injury via down-regulating miR-429.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHongyan Lv,Su Zhang,Xiaohong HaoPubMed 31876239
- Molecular medicine reports 21:123-1302019Sevoflurane attenuates brain damage through inhibiting autophagy and apoptosis in cerebral ischemia‑reperfusion rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCun-Xian Shi,Jin Jin,Xue-Qin Wang,Teng Song,Guang-Hong Li,Ke-Zhong Li,Jia-Hai MaPubMed 31746402
- Experimental and therapeutic medicine 18:4406-44122019Chrysophanol alleviates myocardial injury in diabetic mice by regulating the SIRT1/HMGB1/NF-κB signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPeng Xue et. al.PubMed 31772635
- Artificial cells, nanomedicine, and biotechnology 47:2256-22642019Swainsonine inhibits proliferation and collagen synthesis of NIH-3T3 cells by declining miR-21.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChao Li,Peipei Wang,Ziyang Fu,Yongtao Li,Shouju LiPubMed 31184213
- Chemico-biological interactions 308:364-3712019Notoginsenoside R1 protects human renal proximal tubular epithelial cells from lipopolysaccharide-stimulated inflammatory damage by up-regulation of miR-26a.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJinrong Liu et. al.PubMed 31158334
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human NFKBIA phospho Y42 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P25963
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Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
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Target data
Function
Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL (RELA/p65 and NFKB1/p50) dimers in the cytoplasm by masking their nuclear localization signals (PubMed:1493333, PubMed:36651806, PubMed:7479976). On cellular stimulation by immune and pro-inflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription (PubMed:7479976, PubMed:7628694, PubMed:7796813, PubMed:7878466).
Alternative names
IKBA, MAD3, NFKBI, NFKBIA, NF-kappa-B inhibitor alpha, I-kappa-B-alpha, Major histocompatibility complex enhancer-binding protein MAD3, IkB-alpha, IkappaBalpha
Recommended products
Rabbit Polyclonal IKB alpha phospho Y42 antibody. Suitable for WB and reacts with Human samples. Cited in 21 publications. Immunogen corresponding to Synthetic Peptide within Human NFKBIA phospho Y42 conjugated to Keyhole Limpet Haemocyanin.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human NFKBIA phospho Y42 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P25963
- Purification technique
- Affinity purification Immunogen
- Specificity
There may be some homology to unrelated proteins with a conserved tyrosine phosphorylation motif. It is recommended that this antibody be used after immunoprecipitation with an IKB alpha specific antibody.
- Concentration
- Purification notes
Cross absorbed to phospho-tyrosine coupled to agarose then affinity purified using phospho-IKB alpha (Tyr-42) peptide, without carrier.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
We do not have concentration information for this antibody. It is optimized to work at the dilutions stated on the datasheet.
Useful in detection of phosphorylated IKB alpha (Tyr-42).
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-IKB alpha (phospho Y42) antibody (ab24783)
Western blot analysis of A431 cells treated with pervanadate (1 mM) for 30 minutes (30 ug/lane). Blots were probed with anti-IkappaBalpha (lane 1), anti-phospho-IkappaBalpha?(Tyr-42) (ab24783; lanes 2-5), or anti-phospho-IkappaBalpha (Tyr-305) (Anti-IKB alpha (phospho Y305) antibody ab24784; lanes 6-9). Both anti-phospho-IkappaBalpha (Tyr-42) and anti-phospho-IkappaBalpha (Tyr-305) were used in the presence of no blocking peptide (lane 2 & 6), phospho-IkappaBalpha (Tyr-42) peptide (lane 3 & 8), phospho-IkappaBalpha (Tyr-305) peptide (lane 4 & 7), or BSA conjugated to phospho-tyrosine (lane 5 & 9). Peptides and BSA-pTyr were used at 1 ug/ml.
Lane 1: Western blot - Anti-IKB alpha (phospho Y305) antibody (Anti-IKB alpha (phospho Y305) antibody ab24784)
Lane 1: Western blot - Anti-IKB alpha (phospho Y42) antibody (ab24783)
Predicted band size: 35 kDa
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