JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB92685

Anti-IKB alpha (phospho Y42) antibody [EPR2353]

Be the first to review this product! Submit a review

|

(2 Publications)

Rabbit Recombinant Monoclonal IKB alpha phospho Y42 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications.

View Alternative Names

IKBA, MAD3, NFKBI, NFKBIA, NF-kappa-B inhibitor alpha, I-kappa-B-alpha, Major histocompatibility complex enhancer-binding protein MAD3, IkB-alpha, IkappaBalpha

1 Images
Western blot - Anti-IKB alpha (phospho Y42) antibody [EPR2353] (AB92685)
  • WB

Unknown

Western blot - Anti-IKB alpha (phospho Y42) antibody [EPR2353] (AB92685)

All lanes:

Western blot - Anti-IKB alpha (phospho Y42) antibody [EPR2353] (ab92685) at 1/500 dilution

Lane 1:

A-431 (Human epidermoid carcinoma cell line) whole cell lysate, untreated at 10 µg

Lane 2:

A-431 (Human epidermoid carcinoma cell line) whole cell lysate, pre-treated with pervanadate at 10 µg

Secondary

All lanes:

HRP conjugated Goat anti-Rabbit IgG at 1/2000 dilution

Predicted band size: 35 kDa

false

  • Carrier free

    Anti-IKB alpha (phospho Y42) antibody [EPR2353] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2353

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Alternative Products

Primary Antibodies

AB92700

Anti-IKB alpha (phospho S32) antibody [EPR3148]

20ul selling size|RabMAb|Recombinant

Immunoprecipitation - Anti-IKB alpha (phospho S32) antibody [EPR3148] (AB92700)

  • 5
  • 1
Primary Antibodies

AB133462

Anti-IKB alpha (phospho S36) antibody [EPR6235(2)]

20ul selling size|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-IKB alpha (phospho S36) antibody [EPR6235(2)] (AB133462)

  • 4
  • 1
Primary Antibodies

AB32518

Anti-IKB alpha antibody [E130]

20ul selling size|RabMAb|Recombinant|KO Validated

Western blot - Anti-IKB alpha antibody [E130] (AB32518)

  • 4
  • 31
Primary Antibodies

AB76429

Anti-IKB alpha antibody [EP697]

20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-IKB alpha antibody [EP697] (AB76429)

  • 5
  • 2
Primary Antibodies

AB215972

Anti-IKB alpha antibody [E130] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-IKB alpha antibody [E130] - BSA and Azide free (AB215972)

  • 0
  • 0
Primary Antibodies

AB240059

Anti-IKB alpha (phospho S36) antibody [EPR6235(2)] - BSA and Azide free

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-IKB alpha (phospho S36) antibody [EPR6235(2)] - BSA and Azide free (AB240059)

  • 0
  • 0
Primary Antibodies

AB202646

HRP Anti-IKB alpha antibody [E130]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-IKB alpha antibody [E130] (AB202646)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB32360

Anti-NFkB p105 / p50 antibody [E381]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFkB p105 / p50 antibody [E381] (AB32360)

  • 4
  • 21
Primary Antibodies

AB16502

Anti-NF-kB p65 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NF-kB p65 antibody (AB16502)

  • 4
  • 40
Primary Antibodies

AB124957

Anti-IKK beta antibody [EPR6043]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IKK beta antibody [EPR6043] (AB124957)

  • 5
  • 1
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
style
left-column

Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL (RELA/p65 and NFKB1/p50) dimers in the cytoplasm by masking their nuclear localization signals (PubMed : 1493333, PubMed : 36651806, PubMed : 7479976). On cellular stimulation by immune and pro-inflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription (PubMed : 7479976, PubMed : 7628694, PubMed : 7796813, PubMed : 7878466).
See full target information NFKBIA phospho Y42
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Genes to cells : devoted to molecular & cellular mechanisms 29:337-346 PubMed38329182

2024

Microtubule-affinity regulating kinase family members distinctively affect tau phosphorylation and promote its toxicity in a Drosophila model.

Applications

Unspecified application

Species

Unspecified reactive species

Grigorii Sultanakhmetov,Iori Kato,Akiko Asada,Taro Saito,Kanae Ando

Bioengineered 15:1996016 PubMed34699315

2021

Retracted article: MicroRNA-4521 targets hepatoma up-regulated protein (HURP) to inhibit the malignant progression of breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Changwen Li,Sen Peng,Chuangang Tang
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com