Rabbit Recombinant Monoclonal IKB alpha phospho Y42 antibody. Suitable for WB and reacts with Human samples.
Images
![Western blot - Anti-IKB alpha (phospho Y42) antibody [EPR2353] (AB92685), expandable thumbnail](https://content.abcam.com/products/images/ikb-alpha-phospho-y42-antibody-epr2353-ab92685--western-blot-img46809.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-IKB alpha (phospho Y42) antibody [EPR2353] (AB92685)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Flow Cyt | WB | IHC-P | |
|---|---|---|---|
| Human | Not recommended | Tested | Not recommended |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/1000 | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
7 products for Alternative Product
Target data
Function
Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.
Alternative names
NF-kappa-B inhibitor alpha, I-kappa-B-alpha, Major histocompatibility complex enhancer-binding protein MAD3, IkB-alpha, IkappaBalpha, NFKBIA, IKBA, MAD3, NFKBI
Recommended products
Rabbit Recombinant Monoclonal IKB alpha phospho Y42 antibody. Suitable for WB and reacts with Human samples.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
EPR2353
- Purity
Tissue culture supernatant
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Western blot - Anti-IKB alpha (phospho Y42) antibody [EPR2353] (ab92685), expandable thumbnail](https://content.abcam.com/products/images/ikb-alpha-phospho-y42-antibody-epr2353-ab92685--western-blot-img46809.jpg "Western blot - Anti-IKB alpha (phospho Y42) antibody [EPR2353] (ab92685)")
Western blot - Anti-IKB alpha (phospho Y42) antibody [EPR2353] (ab92685)
All lanes: Western blot - Anti-IKB alpha (phospho Y42) antibody [EPR2353] (ab92685) at 1/500 dilution
Lane 1: A-431 (Human epidermoid carcinoma cell line) whole cell lysate, untreated at 10 µg
Lane 2: A-431 (Human epidermoid carcinoma cell line) whole cell lysate, pre-treated with pervanadate at 10 µg
SecondaryAll lanes: HRP conjugated Goat anti-Rabbit IgG at 1/2000 dilution
Predicted band size: 35 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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