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AB264240

Anti-IKB beta antibody

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Rabbit Polyclonal IKB beta antibody. Suitable for IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human NFKBIB aa 300 to C-terminus.

View Alternative Names

IKBB, TRIP9, NFKBIB, NF-kappa-B inhibitor beta, NF-kappa-BIB, I-kappa-B-beta, Thyroid receptor-interacting protein 9, IkB-B, IkB-beta, IkappaBbeta, TR-interacting protein 9, TRIP-9

2 Images
Immunoprecipitation - Anti-IKB beta antibody (AB264240)
  • IP

Supplier Data

Immunoprecipitation - Anti-IKB beta antibody (AB264240)

IKB beta was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab264240 at 3 μg per reaction. Western blot was performed on the immunoprecipitates using ab264240 at 0.4 μg/ml.

Lane 1 : ab264240 IP in HeLa whole cell lysate.

Lane 2 : Control IgG IP in Hela whole cell lysate.

Exposure time : 30 seconds.

All lanes:

Immunoprecipitation - Anti-IKB beta antibody (ab264240)

Predicted band size: 37 kDa

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Western blot - Anti-IKB beta antibody (AB264240)
  • WB

Supplier Data

Western blot - Anti-IKB beta antibody (AB264240)

All lanes:

Western blot - Anti-IKB beta antibody (ab264240) at 0.04 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

Predicted band size: 37 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human NFKBIB aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q15653

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. However, the unphosphorylated form resynthesized after cell stimulation is able to bind NF-kappa-B allowing its transport to the nucleus and protecting it to further NFKBIA-dependent inactivation. Association with inhibitor kappa B-interacting NKIRAS1 and NKIRAS2 prevent its phosphorylation rendering it more resistant to degradation, explaining its slower degradation.
See full target information NFKBIB
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Product promise

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